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Polyhistidine poly h 5000 d

Manufactured by Merck Group

Polyhistidine (poly H≥5000 D) is a synthetic polymer consisting of multiple histidine amino acid residues. It has a high molecular weight, typically greater than or equal to 5,000 Daltons. The core function of this product is to serve as a protein tag or affinity ligand for the purification and detection of recombinant proteins.

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2 protocols using polyhistidine poly h 5000 d

1

Enzymatic Assays with Peroxidase Conjugates

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Horseradish peroxidase (HRP), microperoxidase (m-POD), ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), Amplex Red, polyhistidine (poly H≥5000 D) and glucose oxidase (GOX) were purchased from Sigma. Hemin was purchased from Alfa Aesar and was prepared in DMSO. Tris Buffered Saline (TBS) and Phosphate Buffered Saline (PBS) were purchased from Fisher.
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2

Enzymatic Assays with Peroxidase Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Horseradish peroxidase (HRP), microperoxidase (m-POD), ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), Amplex Red, polyhistidine (poly H≥5000 D) and glucose oxidase (GOX) were purchased from Sigma. Hemin was purchased from Alfa Aesar and was prepared in DMSO. Tris Buffered Saline (TBS) and Phosphate Buffered Saline (PBS) were purchased from Fisher.
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