Granulosa, COCs, ovarian stromal tissue, or whole ovaries were lysed in RIPA buffer (Boston BioProducts). Proteins were separated using a 10% Bis-Tris gel and transferred to an immobilon-P membrane (Millipore). Primary antibodies used included GAS1 (1:1000 dilution, AF2644; R&D system) and AKT (1:2000 dilution, 9272; Cell Signaling Technology). Signals were detected using the Peirce ECL 2 Western Blotting Substrate (Thermo Scientific).
Peirce ecl 2 western blotting substrate
Manufactured by Thermo Fisher Scientific
The Peirce ECL 2 Western Blotting Substrate is a chemiluminescent detection reagent used in western blotting applications to visualize protein targets. It generates a luminescent signal when exposed to the horseradish peroxidase enzyme, which is commonly used to label antibodies in western blot procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peirce ecl 2 western blotting substrate
1
Ovarian Protein Expression Analysis
2
Western Blot Analysis of ESR1, PR, and p53
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