Total RNA was extracted using the Qiagen Plant RNA Kit, Ambion Turbo RNA-free Kit and approximately 200ng of RNA used for cDNA synthesis with Superscript III reverse transcriptase (Invitrogen) according to the manufacturer’s instructions.
Plant rna kit
Manufactured by Thermo Fisher Scientific
The Plant RNA Kit is a laboratory tool designed to isolate and purify high-quality total RNA from a variety of plant tissues. It utilizes a guanidinium-based lysis buffer and silica-based membrane technology to efficiently capture and wash RNA molecules, enabling users to obtain pure RNA samples for downstream applications such as RT-PCR, Northern blotting, and RNA sequencing.
Automatically generated - may contain errors
2 protocols using plant rna kit
1
Total RNA Extraction and cDNA Synthesis
2
Transcriptome Analysis of Root Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Root samples collected at 0, 9, 24, and 72 h were used for transcriptome analyses. Total RNAs were extracted using the Plant RNA Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States). The samples with RNA integrity number (RIN) ≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, United States) according to the manufacturer’s instructions. Then, these libraries were sequenced on the Illumina Hiseq 4000 Sequencing platform, and 125/150-bp paired-end reads were generated.
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