5 flourouracil 5 fu

Fetal Bovine Serum (FBS) and Phosphate Buffered Saline (PBS) were purchased from Hyclone™, ThermoFisher Scientific, Waltham, MA, USA. HEPES, EDTA, EGTA, TEMED, isopropanol, aprotonin, pepstatin, phenylmethanesulfonyl fluoride (PMSF), leupeptin, sodium fluoride (NaF), sodium orthovanadate (Na₃VO₄), diethyldithiocarbamate (DDC), doxorubicin, etoposide, fludarabine, 5-flouroUracil (5-FU), cisplatin, dimethyl-9,9’-biacridinium dinitrate (Lucigenin), N-acetyl-cysteine (NAC) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Venetoclax (VEN) was purchased from Medchem Express LLC. Streptomycin–penicillin and l-glutamine were purchased from Gibco. Coomassie Blue dye and bovine serum albumins (BSA) were purchased from ThermoFisher Scientific. Methanol and sodium dodecyl sulphate (SDS) were purchased from Merck, Kenilworth, NJ, USA. Sucrose and 30% acrylamide/bis solution were purchased from Bio-Rad. Goat anti-mouse and anti-rabbit IgG horseradish peroxidase conjugated secondary antibodies were purchased from Pierce, TX, USA. Protein A agarose beads were purchased from Santa Cruz, TX, USA. Chloromethyl-2-,7-dichlorofluorescin diacetate (DCFDA) and MitoSox™ were purchased from Molecular Probes, Thermo Fisher Scientific.

Dulbecco’s Modified Eagle Medium (DMEM), Fetal Bovine Serum (FBS), (Gibco, Auckland, New Zealand), RPMI 1640 medium, Penicillin-Streptomycin, MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide), Phosphate-buffered saline (PBS) (Sigma, St. Louis, MO, USA), and DMSO (Dimethyl Sulfoxide) (Merck, Hohenbrunn, Germany), were used to evaluate the cytotoxic activity. Moreover, petroleum ether, dichloromethane, and methanol (Merck, Hohenbrunn, Germany) were used for plant fractionation. The NucView™ 488 Caspase-3 assay kit for live cells (Biotium, Hayward, CA, USA) and Annexin V-FITC Apoptosis Detection kit (BioVision Research Products, Mountain View, CA, USA) were used in apoptosis assays. Also we used 5-Flourouracil (5-FU) (Sigma, St. Louis, MO, USA) as the positive control.