Alphalisa cell lysis buffer

Cell culture medium: Dulbecco’s modified Eagle’s high glucose medium (DMEM-HG), l-glutamine, penicillin/streptomycin, and fetal bovine serum were purchased from Euro-clone (Milan, Italy). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), oleic acid (C18:1), palmitic acid (C16:0), and phosphate-buffered saline (PBS) were obtained from Sigma Chemical (St. Louis, MO, USA). AlphaLISA cell lysis buffer and Alpha Immunoassay buffer were from Perkin Elmer (Boston, MA, USA). Silybin was obtained from Extrasynthese, Genay, France (Cat#1040).

Cell culture medium Dulbecco’s modified Eagle’s high glucose medium (DMEM-HG), L-glutamine, penicillin/streptomycin, fetal bovine serum and EuroGOLD RNA pure were purchased from Euro-clone (Milan, Italy). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), oleic acid (C18:1), palmitic acid (C16:0), phosphate-buffered saline (PBS) were obtained from Sigma Chemical (St. Louis, MO, USA). Reagents for cDNA synthesis and iQ SYBR Green Supermix were from Bio-Rad Laboratories (Hercules, CA, USA). AlphaLISA cell lysis buffer and Alpha Immunoassay buffer were from Perkin Elmer (Boston, MA, USA). Obeticholic acid (INT-747, OCA), INT-767, tropifexor, and selonsertib were kindly provided by Intercept Pharmaceuticals, Inc. (New York, NY, USA).