Dnajb12

Manufactured by Proteintech

DNAJB12 is a lab equipment product from Proteintech. It is a protein that functions as a molecular chaperone, assisting in the folding and unfolding of other proteins.

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Lab products found in correlation

Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Anti mouse igg, by Merck Group (1 mentions) Deoxy big chap, by Merck Group (1 mentions) Hsp90, by Santa Cruz Biotechnology (1 mentions) Anti flag m2 antibody conjugated agarose beads, by Merck Group (1 mentions) Fam134b, by Proteintech (1 mentions) Anti rat igg, by Merck Group (1 mentions) Anti ha magnetic beads, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Normal rabbit and mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti flag tag, by Merck Group (1 mentions)

3 protocols using dnajb12

Immunoprecipitation and Immunofluorescence Protocol

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Deoxy Big CHAP (DBC), 2-mercaptoethanol (BME), phenylmethylsulphonyl fluoride (PMSF), Triton X-100, and anti-FLAG M2 antibody-conjugated agarose beads (A2220) were purchased from Sigma-Aldrich. 16% w/v paraformaldehyde (PFA), Tween 20, glutaraldehyde (2.5%) in sodium cacodylate buffer, and anti-HA magnetic beads (88836) were purchased from Thermo Fisher Scientific. The antibodies used were as follows: FLAG (F1365; Millipore Sigma-Aldrich), RTN4 (sc-271878; Santa Cruz Biotechnology), RTN3 (A302-860A; Bethyl Laboratories), B12 (16780–1-AP; Proteintech Group), Hsp90 (sc7947; Santa Cruz Biotechnology), dsRNA (J2; Jena Biosciences; 10010200), NSP1 (GTX135612; Genetex), Streptavidin (LS-C203628; LifeSpan BioSciences), HA (11867423001; Roche), FAM134B (21537-1-AP; Proteintech Group), BAP31 (MA3-002; Invitrogen), DNAJB12 (16780-1-AP; Proteintech Group), anti-Mouse IgG Alexa Fluor 488 (A-11029; Invitrogen), anti-Rabbit IgG (A4914; Sigma-Aldrich), anti-Mouse IgG (A4416; Sigma-Aldrich), anti-Rat IgG (A5795; Sigma-Aldrich), and ProLong Diamond Antifade Mountant with DAPI (P36962; Invitrogen).

Williams J.M., Chen Y.J., Cho W.J., Tai A.W, & Tsai B. (2023). Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication. The Journal of Cell Biology, 222(7), e202203060.

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Antibody Sources for Protein Analysis

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Polyclonal DnaJB14, DnaJB12, ERdj5, SGTA, Hrd1 were purchased from Proteintech Group (Chicago, IL). An additional rabbit polyclonal against SGTA was provided by Yihong Ye (NIH). Monoclonal BAP31 and polyclonal Hsc70 and Bag6 antibodies were purchased from Pierce (Rockford, IL). Polyclonal Derlin-1 and Sec61α antibodies were provided by Tom Rapoport (Harvard University). Normal rabbit and mouse IgG, polyclonal Hsp90, PDI, and monoclonal SV40 Large T antigen antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit anti-VP1 antibody was a gift from Harumi Kasamatsu (UCLA). Monoclonal VP1 antibody was provided by Walter Scott (University of Miami). Monoclonal p97 was purchased from RDI/Fitzgerald (Concord, MA). Polyclonal BiP and rat anti-Hsc70 antibodies were purchased from Abcam (Cambridge, MA). Polyclonal calnexin antibodies were purchased from Stressgen. Polyclonal ERp29 was a gift from Souren Mkrtchian (Karolinska Institutet).

Walczak C.P., Ravindran M.S., Inoue T, & Tsai B. (2014). A Cytosolic Chaperone Complexes with Dynamic Membrane J-Proteins and Mobilizes a Nonenveloped Virus out of the Endoplasmic Reticulum. PLoS Pathogens, 10(3), e1004007.

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Antibody Panel for Protein Interactions

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Monoclonal SV40 large T antigen antibody (RRID:AB_628305), polyclonal Hsp90 antibody (RRID:AB_2121235) and EMC3 (RRID:AB_10842176) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal VP1 antibody was kindly provided by Walter Scott (University of Miami). Polyclonal anti S-tag (RRID:AB_444553) and BiP (RRID:AB_732737) antibodies were purchased from Abcam (Cambridge, MA), whereas polyclonal anti-VP1 antibody was a gift from Harumi Kasamatsu (UCLA). Polyclonal DnaJ B14 (RRID:AB_2094414), DnaJ B12 (RRID:AB_2094404), and SGTA (RRID:AB_2188830) antibodies were purchased from Proteintech Group (Chicago, IL). Monoclonal BAP31 (RRID:AB_2537133) and polyclonal Hsc70 (RRID:AB_2544813) antibodies were purchased from Pierce (Rockford, IL), and anti-FLAG tag (RRID:AB_439687) antibody was obtained from Sigma (St Louis, MO). Rabbit anti-EMC1 (RRID:AB_10817224) antibody was purchased from Abgent (San Diego, CA). Polyclonal DnaJ C18 antibody was generated by GenScript (Piscataway, NJ). Polyclonal Derlin-1 antibody was provided by Tom Rapoport (Harvard University). Polyclonal CTA antibody was produced against denatured CTA and generated by EMD Biosciences (San Diego, CA).

Bagchi P., Inoue T, & Tsai B. (2016). EMC1-dependent stabilization drives membrane penetration of a partially destabilized non-enveloped virus. eLife, 5, e21470.

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