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Hcs mitochondrial health kit

Manufactured by Thermo Fisher Scientific
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The HCS Mitochondrial Health Kit is a laboratory equipment product designed to assess mitochondrial health in cells. It provides tools for analyzing key mitochondrial functions, including membrane potential, mass, and morphology, using high-content screening (HCS) techniques.

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Lab products found in correlation

Evos m7000, by Thermo Fisher Scientific (2 mentions) Celleste image analysis software, by Thermo Fisher Scientific (2 mentions) Folin ciocalteu s phenol reagent, by Merck Group (1 mentions) Cellrox oxidative stress reagent, by Thermo Fisher Scientific (1 mentions) Pepsin, by Merck Group (1 mentions) Pancreatin, by Merck Group (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Lsm700 meta confocal laser microscope, by Zeiss (1 mentions)

4 protocols using hcs mitochondrial health kit

1

Mitochondrial Function Assay of Cells

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The mitochondrial functionality was analyzed by HCS Mitochondrial Health Kit (Thermo Fisher Scientific), following the manufacturer’s procedures. Briefly, hFM-MSCs and hAFSCs were treated with 0.1 μM of BPA, BPS, PFOS, and PFOA, alone or in combination, for 24 h. Then, cells were incubated with the MitoStain for 30 min at 37 °C and then fixed in paraformaldehyde 4% for 10 min. Nuclei were counterstained by Hoechst 33342. To quantify the fluorescence, 4 different fields for each sample were acquired by EVOS M7000 (Thermo Fisher Scientific) and analyzed by Celleste Image Analysis Software (Thermo Fisher Scientific).
Di Credico A., Gaggi G., Bucci I., Ghinassi B, & Di Baldassarre A. (2023). The Effects of Combined Exposure to Bisphenols and Perfluoroalkyls on Human Perinatal Stem Cells and the Potential Implications for Health Outcomes. International Journal of Molecular Sciences, 24(19), 15018.
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2

Jackfruit Bioavailability and Oxidative Stress

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Jackfruits were obtained from Hainan Province, China. Acrylamide was purchased from Aladin. Pepsin (2000 U/mL), pancreatin (800 U/mL), bile, dulbecco’s modified eagle medium (DMEM) culture media, and Folin–Ciocalteu’s phenol reagent were purchased from Sigma Chemical (St Louis, MO, USA). Caco-2 was obtained from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences (Hangzhou, China). Hoechst 33342 and PI were purchased from Beyotime Institute of Biotechnology (Shanghai, China). HCS Mitochondrial Health Kit and Cell ROX® Oxidative Stress Reagent were purchased by Thermo Scientific (San Jose, CA, USA). All other reagents were of analytical grade.
Qu D., Liu C., Jiang M., Feng L., Chen Y, & Han J. (2019). After In Vitro Digestion, Jackfruit Flake Affords Protection against Acrylamide-Induced Oxidative Damage. Molecules, 24(18), 3322.
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3

Simultaneous Mitochondrial and Cytotoxicity Assay

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The HCS Mitochondrial Health Kit (H10295, Thermo Fisher Scientific) was used to measure mitotoxicity and cytotoxicity simultaneously in the same cell. Briefly, cell staining solution was prepared by adding 10.5 μl of the MitoHealth stain solution and 2.1 μl of Image-iT® DEAD Green™ viability stain to 6 ml complete medium according to the manufacturer’s protocol. The neuronal cultures were incubated with the cell staining solution for 30 min under culture conditions, followed by adding the counterstain/fixation solution for 15 min at room temperature. The red MitoHealth stain corresponding to mitochondrial membrane potential (MMP) and DEAD Green (cytotoxicity) were captured under a LSM700 Meta confocal laser microscope (Carl Zeiss).
Wang R., Dong Y., Lu Y., Zhang W., Brann D.W, & Zhang Q. (2018). Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions. Molecular neurobiology, 56(3), 1852-1869.
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4

Mitochondrial Function Assay of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mitochondrial functionality was analyzed by HCS Mitochondrial Health Kit (Thermo Fisher Scientific), following the manufacturer’s procedures. Briefly, hFM-MSCs and hAFSCs were treated with 0.1 μM of BPA, BPS, PFOS, and PFOA, alone or in combination, for 24 h. Then, cells were incubated with the MitoStain for 30 min at 37 °C and then fixed in paraformaldehyde 4% for 10 min. Nuclei were counterstained by Hoechst 33342. To quantify the fluorescence, 4 different fields for each sample were acquired by EVOS M7000 (Thermo Fisher Scientific) and analyzed by Celleste Image Analysis Software (Thermo Fisher Scientific).
Di Credico A., Gaggi G., Bucci I., Ghinassi B, & Di Baldassarre A. (2023). The Effects of Combined Exposure to Bisphenols and Perfluoroalkyls on Human Perinatal Stem Cells and the Potential Implications for Health Outcomes. International Journal of Molecular Sciences, 24(19), 15018.
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