Szx10 stereoscope

Manufactured by Olympus

Sourced in Japan

The SZX10 stereoscope is a high-quality optical microscope designed for a variety of laboratory applications. It features a zoom range that allows for flexible magnification and observation of specimens. The SZX10 provides a clear, high-resolution image to facilitate detailed analysis and examination.

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Lab products found in correlation

Crystal violet solution, by Merck Group (1 mentions) Illustrator cs6, by Adobe (1 mentions)

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SZX10 (258 citations) SZX10 fluorescent stereoscope (2 citations)

4 protocols using szx10 stereoscope

Microplastic Characterization Protocol

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Filters were visually inspected under a microscope (Olympus SZX10 stereoscope) with a camera attachment (Olympus SC50). Microparticles were categorized by morphotype and color, then measured using cellSens software (length or area; OLYMPUS cellSens Entry 2.3). Only microparticles 330 to 5000 µm (length; Masura et al., 2015 ) were categorized and measured due to the resolution of the microscope and camera.
During visual inspection, it is nearly impossible to determine polymer composition; therefore, all particles that appeared to be of anthropogenic origin were counted and categorized by morphotype and color (Figure 1). Morphotypes included fibers, foils, and fragments. Particle color was recorded as the primary color found on the majority of the particle (some particles had multiple colors but only one color was recorded for the purpose of analyses). Rare colors (<1% of total observations) were recorded and categorized as “other” for analyses.

Harris L.S., La Beur L., Olsen A.Y., Smith A., Eggers L., Pedersen E., Van Brocklin J., Brander S.M, & Larson S. (2021). Temporal Variability of Microparticles Under the Seattle Aquarium, Washington State: Documenting the Global Covid‐19 Pandemic. Environmental Toxicology and Chemistry, 41(4), 917-930.

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Clonogenic Assay for Cell Survival

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Directly after irradiation or mock-irradiation, cells were collected by trypsinization and seeded at optimized cell seeding densities (500–3000 cells/well) in 6-well plates and incubated at standard conditions for 6–12 days. Cells were fixed with 10% neutral buffered formalin and stained with 0.01% Crystal violet solution (Sigma-Aldrich, # V5265, Castle Hill, Australia) for 1 h. Excess stain was removed with H₂O and once dried, colonies were counted under a stereo microscope (SZX10 Stereoscope, Olympus, Tokyo, Japan). Three independent experiments with three technical replicates were performed. Plating efficiency (PE) and surviving fraction (SF) were calculated as described previously [7 (link)].

Butz F., Eichelmann A.K., Mayne G.C., Wang T., Bastian I., Chiam K., Marri S., Sykes P.J., Wijnhoven B.P., Toxopeus E., Michael M.Z., Karapetis C.S., Hummel R., Watson D.I, & Hussey D.J. (2020). MicroRNA Profiling in Oesophageal Adenocarcinoma Cell Lines and Patient Serum Samples Reveals a Role for miR-451a in Radiation Resistance. International Journal of Molecular Sciences, 21(23), 8898.

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Collecting and Preparing S. holzenthali Specimens

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The specimen of S.holzenthali sp. nov. was collected using a UV light trap and fixed in 96% ethyl alcohol. Specimen preparation and observation was done following standard methods outlined in Blahnik and Holzenthal (2004) . The male genitalia were prepared using 80% lactic acid at 90 °C for 1 h. The specimen was examined on an Olympus SZX10 stereoscope, and pencil sketches were made using a drawing tube attached to a Leitz Dialux 20 compound microscope. The pencil sketches were then scanned using a Konica Minolta bizhub C368 multifunction printer and imported into Adobe Illustrator CS6 to serve as a template for the digital illustration.
The distribution map (Fig. 1) was prepared in QGIS 3.22.4 Białowieża (QGIS Development Team, 2022). Vector and raster maps were prepared with Natural Earth (2018) and CIAT-CSI SRTM (Jarvis et al. 2008 ) data.
All specimens treated in this paper are stored in 96% ethyl alcohol and are deposited in the Senckenberg Research Institute and Natural History Museum, Frankfurt, Germany (

SMF

Rázuri-Gonzales E., Ngera M.F, & Pauls S.U. (2022). A new species of Silvatares (Trichoptera, Pisuliidae) from the Democratic Republic of the Congo. ZooKeys, 1111, 371-380.

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Assessing Motility of M. xanthus under Temperature Variations

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M. xanthus was grown in CTT overnight to mid-log growth phase, harvested, washed with TPM buffer, and adjusted to a cell density of 6 × 10⁸ cfu ml^–1 and spotted on CTT with 1.5 or 0.5% agar to assess A- and S-motility, respectively. Micrographs were taken at various times following incubation at 24°C (room temperature) or 33°C (optimal growth temperature) using a Nikon E800 phase-contrast compound microscope or an Olympus SZX10 stereoscope coupled to imaging systems.
To determine the effect of temperature changes on S-motility, cells grown in CTT medium with 0.5 mM CaCl₂ were spotted on CTT with 2 mM CaCl₂ 0.5% agar-coated glass slides and incubated in a humid chamber at indicated temperatures and times. For time-lapse videos, culture inoculums were similarly dried on agar pads and viewed with an Olympus IX83 inverted microscope equipped with a 60 × oil immersion objective lens coupled to an Orca-Flash4.0 LT sCMOS camera.

Subedi K, & Wall D. (2022). Conditional and Synthetic Type IV Pili-Dependent Motility Phenotypes in Myxococcus xanthus. Frontiers in Microbiology, 13, 879090.

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