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Dmi8 microscope

Manufactured by Nikon
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The DMi8 microscope is a high-quality laboratory instrument manufactured by Nikon. It is designed for a wide range of microscopy applications. The DMi8 offers advanced optical and imaging capabilities to support scientific research and analysis.

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Lab products found in correlation

Mouse on mouse elite peroxidase kit, by Vector Laboratories (1 mentions) Ti2 inverted microscope, by Nikon (1 mentions)

3 protocols using dmi8 microscope

1

Immunohistochemical and Fluorescent Analysis

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Immunohistochemical analysis of formalin‐fixed, paraffin‐embedded/cryosectioned skin/tumour tissues, were performed using Mouse on Mouse Elite Peroxidase Kit (Vector Labs). Alternatively, tissues were subjected to direct/indirect immunofluorescence studies. Bright field and fluorescent images were captured using Leica DMi8 microscope and Nikon eclipse confocal microscope, respectively.
Shankar G. M., Alex V.V., Nisthul A. A., Bava S.V., Sundaram S., Retnakumari A.P., Chittalakkottu S, & Anto R.J. (2019). Pre‐clinical evidences for the efficacy of tryptanthrin as a potent suppressor of skin cancer. Cell Proliferation, 53(1), e12710.
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2

Screening Neuron-Specific Protein Localization

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A strain expressing mNeonGreen::ESYT-2 in DA9 neuron (sybIs50) was mutagenized with EMS according to standard protocols (Anderson, 1995 (link)). 1000 F1s were cloned into different plates, and 30–50 F2 animals from individual F1 animals were subjected to a direct visual screen under a microscope. The mutants were isolated based on the changes in mNeonGreen::ESYT-2 distribution in DA9 neuron as observed with a 40x objective on either a Leica DMi8 microscope or a Nikon Ti2 inverted microscope.
Sun J., Harion R., Naito T, & Saheki Y. (2021). INPP5K and Atlastin-1 maintain the nonuniform distribution of ER–plasma membrane contacts in neurons. Life Science Alliance, 4(11), e202101092.
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3

Screening Candida albicans Growth

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Spider medium agar plates (1% peptone, 1% mannitol, 0.2% K2HPO4 and 1.5% agar) were supplemented with different concentrations of compounds as indicated. C. albicans SC5314 cells were grown in the plates at 37°C for 24–30 h. Images of the colonies were obtained using a Leica DMi8 microscope and a Nikon Coolpix digital camera.
Zhao S., Huang J., Sun X., Huang X., Fu S., Yang L., Liu X., He F, & Deng Y. (2018). (1‐aryloxy‐2‐hydroxypropyl)‐phenylpiperazine derivatives suppress Candida albicans virulence by interfering with morphological transition. Microbial Biotechnology, 11(6), 1080-1089.
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