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Phosphate buffer saline pbs ph 7.4

Manufactured by Solarbio
Sourced in China

Phosphate buffer saline (PBS; pH 7.4) is a widely used buffer solution in various laboratory applications. It is an aqueous solution consisting of sodium phosphate and sodium chloride, maintaining a neutral pH of 7.4. PBS is designed to maintain the physiological conditions necessary for the survival and activity of biological samples.

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3 protocols using phosphate buffer saline pbs ph 7.4

1

Cytotoxicity Evaluation of Chemical Reagents

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Urea, citric acid, and hydrochloric acid were purchased from Sinopharm Group Chemical Reagent (Shanghai, China). Cell counting kit 8 (CCK-8), Hoechst 33342, propidium iodide (PI), trypsin, penicillin/streptomycin, and phosphate buffer saline (PBS; pH 7.4) were obtained from Solarbio (Beijing, China). Calcein acetoxymethyl (AM) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and RPMI-1640 medium were purchased from Yuanpei Biotechnology Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS) was purchased from PAN Seratech (Leica TCS SP8, Wetzlar, Germany).
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2

Curcumin-Loaded Lipid Nanoparticles

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ABZ was obtained as a gift sample from Vetindia Pharmaceuticals Limited (Hyderabad, Andhra Pradesh, India). Curcumin, phosphate buffer saline (PBS, pH 7.4) and glyceryl trimyristate were purchased from Solarbio Life Sciences (Beijing, China). Tween 80 and all solvents used in this study were acquired from Tianjin Chemical and Reagents (Tianjin, China).
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3

Synthesis and Characterization of PMMA-co-MAA

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Poly(methyl methacrylate-co-methacrylic acid) (PMMA-co-MAA), Mw: 34,000, molar proportion of methyl methacrylate to methacrylic acid is 1:0.016) was purchased from Sigma-Aldrich Corporation. Phosphate buffer saline (PBS, pH 7.4) solution was purchased from Beijing Solarbio Technology Corporation. All other chemicals of analytical grade were used as received. All aqueous solutions were prepared using deionized water with the conductivity of 18 MΩ cm.
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