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Normocin ant nr 1

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Normocin is an antibiotic mixture composed of three components: Neomycin, Paromomycin, and Trimethoprim. It is intended for use in cell culture applications to prevent microbial contamination.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Lncap clone fgc, by American Type Culture Collection (1 mentions) Dmem 11965092, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Taq dna ligase, by New England Biolabs (1 mentions) Puromycin ant pr 1, by InvivoGen (1 mentions) Blasticidin ant bl 1, by InvivoGen (1 mentions) Zeocin ant zn 1, by InvivoGen (1 mentions) Phusion high fidelity dna polymerase, by New England Biolabs (1 mentions) T5 exonuclease, by New England Biolabs (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions) C 22062, by PromoCell (1 mentions) P0781, by Merck Group (1 mentions) F7524, by Merck Group (1 mentions) Microbeta2, by PerkinElmer (1 mentions) Neolite reagent, by PerkinElmer (1 mentions)

5 protocols using normocin ant nr 1

1

Cell Culture Protocols for Various Cell Lines

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Human HEK293T cells (obtained from ATCC) and human GCs of the KGN line (obtained from BIUEFBIO) were grown in Dulbecco’s minimal Eagle medium (DMEM, 11,965-092; Gibco, Grand Island, NY, USA), the human prostate cancer cell line (LNCaP clone FGC, obtained from ATCC) was cultured in RPMI-1640 (61870036; Gibco), and mouse GCs of the GRM02 line (obtained from BIUEFBIO) were maintained in DMEM/F12 (1:1) medium (L310KJ; BasalMedia, Shanghai, China) supplemented with 10% (vol/vol) foetal bovine serum (FBS, A3161002C; Gibco), 1% (vol/vol) penicillin/streptomycin (15140-122; Gibco) and 2% normocin (ant-nr-1; InvivoGen, San Diego, CA, USA) at 37°C in 5% CO2 with 100% humidity.
Xue T., Zhao S., Zhang H., Tang T., Zheng L., Jing J., Ge X., Ma R., Ma J., Ren X., Jueraitetibaike K., Guo Z., Chen L, & Yao B. (2023). PPT1 regulation of HSP90α depalmitoylation participates in the pathogenesis of hyperandrogenism. iScience, 26(3), 106131.
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2

Comprehensive Chemical Reagents for Cell Studies

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The following chemicals and reagents were used in this study: torin1 (sc-396760, Santa Cruz, Dallas, TX), BafA1 (tlrl-baf1, InvivoGen, San Diego, CA), poly-l-lysine (P4707-50ML, MilliporeSigma), Lipofectamine 3000 (L3000008, Thermo Fisher Scientific), nucleofector kit T (VACA-1002, Lonza, Basel, Switzerland), polybrene (H9268-5G, MilliporeSigma), normocin (ant-nr-1, InvivoGen), puromycin (ant-pr-1, InvivoGen), blasticidin (ant-bl-1, InvivoGen), zeocin (ant-zn-1, InvivoGen, San Diego, CA), normal goat serum (NGS; ab7481, Abcam, Cambridge, United Kingdom), Phusion High-Fidelity DNA polymerase (M0530L, NEB, Ipswich, MA), T5 exonuclease (M0363S, NEB, Ipswich, MA), and Taq DNA ligase (M0208L, NEB, Ipswich, MA).
Shoemaker C.J., Huang T.Q., Weir N.R., Polyakov N.J., Schultz S.W, & Denic V. (2019). CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor. PLoS Biology, 17(4), e2007044.
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3

Cultivation of SARS-CoV-2 related cell lines

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Vero E6 (ATCC® CRL-1596) cells were cultivated in Dulbecco's modified Eagle’ medium (DMEM, 11995065, Gibco®, Life technologies, Europe B.V) supplemented with 10 % Fetal Bovine Serum (FBS, 10082147, Gibco®), 1 % 10 mM HEPES in 0.85 % NaCl (17-737E, Lonza, BioWhittaker®, Walkersville, MD, USA), 1 % antibiotic-antimycotic, penicillin 10 U/mL, streptomycin 100 µg/mL, and Fungizone™ (amphotericin B) 0.25 µg/mL (15240062, Gibco™). A549 lung carcinoma cells expressing human ACE2 (M08-0801, ©InvivoGen, San Diego, USA) were cultivated in DMEM (Gibco®) supplemented with 10 % FBS (10082147, Gibco®), 1 % 10 mM HEPES in 0.85 % NaCl (17-737E, Lonza), 100 U/mL penicillin, 100 µg/mL streptomycin (15140-12, Gibco®), and 100 µg/mL Normocin™ (ant-nr-1, ©InvivoGen). Cells were maintained at 37°C with 5 % CO2.
Lim T., Rajoriya S., Kim B., Natasha A., Im H., Shim H.S., Yoo J., Kim J.W., Lee E.W., Shin H.J., Kim S.H, & Kim W.K. (2024). In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses. Virus Research, 342, 199325.
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4

Isolation and Culture of Mouse Fibroblasts

Cited 1 time
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Primary mouse embryonic fibroblasts (MEFs) were isolated from wild-type (WT) and AOX-transgenic (AOXRosa26) [7 (link)] embryos (E13.5–15.5) in the C57BL/6 background and immortalized (generating iMEFs) as described elsewhere [35 (link), 36 (link)]. MEFs, iMEFs, and human lung carcinoma (A549) cells (86012804, Sigma-Aldrich/Merck Life Science) were grown in Dulbecco Modified Eagle’s Medium (DMEM) supplemented with 4.5 g/l glucose (BE12-614F, Lonza/BioNordika), 10% fetal bovine serum (FBS, 10270106, Gibco/ThermoFisher Scientific), 100 U/ml penicillin plus 100 μg/ml streptomycin (P0781, Sigma-Aldrich/Merck Life Science), and 2 mM L-glutamine (BE17-605E, Lonza/BioNordika). Mouse pulmonary artery smooth muscle cells (PASMCs) were isolated from precapillary pulmonary arterial vessels of WT C57Bl/6 mice as previously described [37 (link)]. PASMCs were cultured in Smooth Muscle Cell Growth Medium (C-22062, PromoCell) supplemented with 10% FCS (F7524, Sigma-Aldrich/Merck Life Science) and 0.002% Normocin (ant-nr-1, InvivoGen).
Giordano L., Aneja M.K., Sommer N., Alebrahimdehkordi N., Seraji A., Weissmann N., Rudolph C., Plank C., Jacobs H.T, & Szibor M. (2021). Alternative oxidase encoded by sequence-optimized and chemically-modified RNA transfected into mammalian cells is catalytically active. Gene Therapy, 29(12), 655-664.
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5

RET-dependent MAPK Activation by GFRa1

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RET-dependent activation of mitogen-activated protein kinase by soluble GFRa1 was measured using MG87 fibroblasts that stably express RET (MG87RET) and the PathDetect-Elk1 system (219005; Stratagene). One day before the assay, cells were plated in a 96-well plate (20,000 cells/well) in 100 μL/well Dulbecco's modified Eagle medium containing 10% fetal bovine serum and 100 μg/mL normocin (ant-nr-1; InvivoGEN) and cultured overnight in an incubator with 5% CO2. Soluble GFRa1 (1–5000 ng/mL, 560-GR; R&D Systems) and GDNF (100 ng/mL 450-10; Peprotech) were added to wells in quadruplicate per GFRa1 concentration. The plates then were cultured for an additional 22–24 hours for luciferase expression, after which the culture media was discarded and the cells were incubated with NeoLite reagent (6016711; Perkin Elmer) for 10 minutes. Luciferase activity was measured using a MicroBeta 2 (PerkinElmer) plate counter. Each experiment was performed twice, and the results were analyzed using GraphPad Prism software.
Porokuokka L.L., Virtanen H.T., Lindén J., Sidorova Y., Danilova T., Lindahl M., Saarma M, & Andressoo J.O. (2018). Gfra1 Underexpression Causes Hirschsprung’s Disease and Associated Enterocolitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 7(3), 655-678.
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