Dulbecco’s Modified Eagle’s Medium (DMEM, with high or low glucose), fetal bovine serum (FBS), L-glutamine, penicillin and streptomycin, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl formazan (MTT), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid hydrate (Ferrozine®), Folin-Ciocalteau reagent, gallic acid, ascorbic acid, Oil red O solution, Coomassie Brilliant Blue G-250, gelatin, and all chemicals and solvents were purchased from Merck KGaA (Darmstadt, DA, Germany). Electrophoresis reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Primary antibodies were supplied by Cell Signaling Technology (Beverly, MA, USA), Molecular ProbesTM (Invitrogen, Carlsbad, CA, USA), and Santa Cruz (Heidelberg, Germany) (Table 3 ). HRP-linked anti-mouse IgG and anti-rabbit IgG secondary antibodies were obtained from Molecular ProbesTM (Invitrogen, Carlsbad, CA, USA). Disposable plastic was provided by Sarstedt (Milan, Italy).
Hrp linked anti mouse igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
HRP-linked anti-mouse IgG is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) in various immunoassays and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Hrp linked anti rabbit igg, by Cell Signaling Technology (2 mentions)
Penicillin and streptomycin, by Merck Group (1 mentions)
Coomassie brilliant blue g 250, by Merck Group (1 mentions)
Oil red o solution, by Merck Group (1 mentions)
Molecular probestm, by Thermo Fisher Scientific (1 mentions)
Anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
Electrophoresis reagents, by Bio-Rad (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Folin ciocalteau reagent, by Merck Group (1 mentions)
Blocking buffer, by Thermo Fisher Scientific (1 mentions)
Nylon membrane, by Thermo Fisher Scientific (1 mentions)
Supersignal west femto, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rabbit monoclonal anti gapdh, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti flag m2, by Merck Group (1 mentions)
Recombinant human ifn α, by Thermo Fisher Scientific (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
4 protocols using hrp linked anti mouse igg
1
Comprehensive Cell Culture Protocol
2
Northern Blot Analysis of Planthopper Viruses
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Total RNA was extracted from nonviruliferous and viruliferous planthoppers, separated on 15% (wt/vol) denaturing polyacrylamide gels, and electroblotted onto nylon membranes (Invitrogen). Digoxigenin (DIG)-labeled LNA oligonucleotide probes (20 ng/mL, RiboBio, Guangzhou, China) were generated for the antisense sequence of vsR-1524, vsR-3397, and U6 snRNA (5’-CTAATCTTCTCTGTATCGTTCC-3’). Probe hybridizations were performed at 43°C for vsR-3397 and 37°C for vsR-1524 or U6. The membranes were blocked with blocking buffer (Invitrogen) for 15 min followed by incubation with an anti-DIG monoclonal antibody (1:500, MBL, Nagoya, Japan) for 1 h and then with a HRP-linked anti-mouse IgG (1:500, Invitrogen) for 1 h. Detection was carried out using SuperSignal West Femto (Thermo Fisher Scientific).
3
Interferon Signaling Pathway Regulation
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HEK293T cells (ATCC® CRL-3216™) were grown in Dulbecco’s modified Eagle’s medium (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Sigma, St. Louis, MO, USA). Cells were incubated at 37 °C in a humidified 5% CO2 atmosphere. Plasmid pISRE-luc was kindly provided by Prof. Ian Goodfellow. Plasmid pcDNA3-V5-VP24 was a kind gift of Dr. St Patrick Reid. pcDNA3-NS1 was kindly given by Prof. Stephan Ludwig. pRL-TK was purchased from Promega (Madison, WI, USA). T-Pro P-Fect Transfection Reagent was from T-Pro Biotechnology (Taipei, Taiwan R.O.C.). Human Recombinant IFN-α was purchased from PeproTech (London, UK). Mouse monoclonal anti-FLAG M2 was obtained from Sigma, and rabbit monoclonal anti-GAPDH and HRP-linked anti-rabbit IgG were purchased from Cell Signaling (Danvers, MA, USA). HRP-linked anti-mouse IgG was provided by Invitrogen (Carlsbad, CA, USA). Pierce ECL Western Blotting Substrate was from Thermo Fisher Scientific (Waltham, MA, USA).
4
Zebrafish Brain Proteome Analysis
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Zebrafish brains were dissected from adults and flash frozen in liquid nitrogen before storing at −80° C. Frozen brains were homogenised in 100 μl lysis buffer consisting of 50 mM Tris-HCl (pH 7.4), 10 mM sodium glycerophosphate, 10 mM sodium pyrophosphate, 150 mM NaCl, 5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 1% Triton X-100, 10% glycerol, 1 mM sodium orthovanadate and cOmplete™Mini EDTA-free Protease Inhibitor Cocktail (Roche). The lysate was centrifuged at 21,000 g for 10 min prior to running on a 12% SDS-PAGE gel. After transfer to a PVDF membrane, immunodetection used the anti-Dj-1 polyclonal antibody (PA5-72638, Invitrogen) and anti-Gapdh monoclonal antibody (G8795, Sigma-Aldrich), both at a dilution of 1:50,000. Horseradish peroxidase (HRP)-linked anti-rabbit IgG (7074, Cell Signaling Technology) and HRP-linked anti-mouse IgG (62-650, Invitrogen) were used at concentrations of 1:2000 and 1:4000, respectively.
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