Cell culture grade dmso

Human hepatocytes, HSCs, Kupffer cells, LSECs and Liver Bio Kits were purchased directly from Emulate, Inc. (Boston, MA). The cells were kept frozen in liquid N₂ storage (vapor phase) until they were cultured. All reagents were aliquoted at appropriate volumes prior to use to avoid multiple freeze-thaw cycles. Fibronectin (Thermo Fisher: Waltham, MA) was resuspended in cell culture grade water to a concentration of 1 mg/mL. Matrigel (Corning; Corning, NY) was thawed overnight on slushy ice in a 4 ֯C refrigerator. Using cold pipette tips, Matrigel was aliquoted to 5 mg aliquots based on the specific stock concentration. L-ascorbic acid (Sigma-Aldrich; St. Louis, MO) was resuspended in cell culture grade water to a concentration of 50 mg/mL. Dexamethasone (Sigma-Aldrich) was resuspended in cell culture grade DMSO (Thermo Fisher) at concentrations of 1 mM and 10 mM. All reagent aliquots were stored at -20 ֯C. The VX used in this study was synthesized and purified by U.S. Army DEVCOM Chemical Biological Center chemists in accordance with international guidelines. VX stock solutions were prepared by dissolving compound in cell culture grade DMSO (Sigma-Aldrich) at a concentration of 5 mM.

HPßCD (2-hydroxy Propyl-β-Cyclodextrin) was purchased from Sigma-Aldrich (St. Louis, MO), cell culture media was from Thermo Scientific (Waltham, MA), and fetal bovine serum was purchased from Hyclone (Logan, Utah). HET0016 was obtained from Dr. JR Falck of UT Southwestern University, Texas, and also synthesized by Dr. Iryna Lebedyeva in the department of Chemistry and Physics, Augusta University with a purity of more than 97%. Cell culture grade DMSO was purchased from Fischer Scientific (PA). Blood chemistry profiles and electrolytes were determined Using third party vendor (Antech Diagnostic).

HPßCD (2-hydroxy Propyl-β-Cyclodextrin) was purchased from Sigma-Aldrich (St. Louis, MO), cell culture media was from Thermo Scientific (Waltham, MA), and fetal bovine serum was purchased from Hyclone (Logan, Utah). HET0016 was made by Dr. Levedyeva in the Department of Chemistry, Augusta University with a purity of more than 97%, and was prepared for animal treatment according to our previously described method [8 (link)]. Additional information for HET0016 synthesis strategies is provided in the S1 File. Cell culture grade DMSO was purchased from Fischer Scientific (Pittsburg, PA). the complex of HET0016 plus HPßCD was made as per previous publication [8 (link)]. VEGFR2 tyrosine kinase inhibitor (Vatalanib) and colony-stimulating factor 1 receptor (CSF1R) inhibitor (GW2580) were purchased from LC Laboratories, Woburn, MA. SB225002 (CXCR2 inhibitor) was purchased from Selleckchem, Houston, TX. Navarixin was purchased from MedKoo bioscience Inc, Morrisville, NC. All flow antibodies are from Bio Legend, San Diego, CA. All antibodies for western blotting, immunohistochemistry, and immunofluorescence were purchased from Santa Cruz (total-CXCR2 and anti-GAPDH), R&D Systems (anti-hCXCR2), Thermo Scientific (anti-Laminin), and Sigma Aldrich (β-actin and FITC-conjugated tomato lectin). All culture media were purchased from Corning and GE Healthcare Life Sciences.