Uas gfpnls

Manufactured by BD

Sourced in United States

The UAS-GFPnls is a laboratory equipment product developed by BD. It is a molecular biology tool that allows for the expression of a nuclear-localized green fluorescent protein (GFP) in various cell types. The core function of the UAS-GFPnls is to facilitate the visualization and study of cellular processes and gene expression patterns within the nucleus.

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Lab products found in correlation

Ey flp, by BD (1 mentions) Uas dcr2, by BD (1 mentions) Uas p35, by BD (1 mentions) Apgal4, by BD (1 mentions) Uas src gfp, by BD (1 mentions) Uas myrrfp, by BD (1 mentions) Phmgal4, by BD (1 mentions) Fkhgal4, by BD (1 mentions) Uas rheb, by BD (1 mentions) Uas cd8 gfp, by BD (1 mentions) Uas sce rnai, by BD (1 mentions) Bam gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas sa rnai, by BD (1 mentions)

3 protocols using uas gfpnls

Drosophila Genetic Tools for Optix Studies

Cited 2 times

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Drosophila lines were obtained from the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila RNAi Center (VDRC): UAS-Optix-RNAi (VDRC-KK110813), UAS-Dcr-2 (VDRC-60008), tsh[md621]-GAL4 (BDSC-3040), dpp[blk1]-GAL4 (BDSC-1553), UAS-Dcr-2; ey-FLP (BDSC-5580), UAS-GFP-nls (BDSC-4776), ato^5’EYE-GAL4 (Yu et al., 2015 (link)), the G-TRACE line UAS-FLP Ubi-p63E(FRT.STOP)Stinger (BDSC-28282; Evans et al., 2009 (link)) and Actin5C>y+>GAL4 (BDSC-3953); the latter two are referred to as UAS-FLP Ubi>IC>GFP and Act>IC>GAL4, respectively, in the text and figures (IC=interruption cassette). For reporter constructs, genomic DNA fragments were cloned into the vectors pCasper-β-gal (encoding cytoplasmic β-Galactosidase) or pStinger (encoding nuclear eGFP) (Pirrotta, 1988 (link); Barolo et al., 2000 ). Both vectors were first modified to contain a 1.1 kb fragment spanning the ato promoter region and 5’UTR (Sun et al., 1998 (link)). All constructs were confirmed by sequencing (data are available upon request) and injected into w CantonS for P-element transformation (Spradling and Rubin, 1982 (link)). All crosses for Fig. 4 were carried out at 30°C to enhance Optix gene silencing.

Zhou Q., DeSantis D.F., Friedrich M, & Pignoni F. (2016). Shared and distinct mechanisms of atonal regulation in Drosophila ocelli and compound eyes. Developmental biology, 418(1), 10-16.

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Drosophila Genetic Tools and Strains

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All fly stocks were reared at 25°C on standard flour/agar Drosophila media. The Gal4/UAS system [58 (link)] was used to drive the expression of transgenes at 29°C. The following strains were provided by the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila RNAi Center (VDRC): Df(3R)BSC503 (BDSC 25007); Df(3R)ED6332 (BDSC 24141); hdc⁴³(BDSC 64063); hdc⁵⁰(BDSC 64064); hdc^BG23007(BDSC 12410); UAS-hdc (BDSC 64056); hdc^RI(VDRC 104322); hdc^R2(VDRC 45069); hdc^R3(BDSC 30489); UAS-p35 (BDSC 5073); UAS-Rheb (BDSC 9689); UAS-Xbp1-EGFP (BDSC 60731); Xbp1^RI(BDSC 36755); PEK^RI(VDRC 110278); UAS-myristoylated-Tomato (BDSC 32222); UAS-CD8-GFP (BDSC 5137); UAS-src-GFP (BDSC 5429); UAS-myrRFP (BDSC 7138); hsp70-GFP (BDSC 51354); phmGal4 (BDSC 80577); fkhGal4 (BDSC 78060); apGal4 (BDSC 3041), UAS-GFPnls (BDSC 4776); TM3-cherry (BDSC 35524); puc-LacZ (BDSC 11173); The following strains are described in Flybase: nub-Gal4 [59 (link)]; Ci-Gal4 [60 (link)]; sal^E/PV [61 (link)]; amn^c651 [10 (link)]. Gal4 drivers were recombined to UAS fluorescent markers described here. UAS-SOD1::UAS-Cat recombined construct was kindly provided by F. Serras. phmGal4::YPetAtet [16 (link)] was kindly provided by X. Franch-Marro. w¹¹⁸ strain was used as control.

Giannios P, & Casanova J. (2021). Systemic and local effect of the Drosophila headcase gene and its role in stress protection of Adult Progenitor Cells. PLoS Genetics, 17(2), e1009362.

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Drosophila Genetics for Protein Degradation

Cited 6 times

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Flies were maintained on standard cornmeal/agar food at 25°C, unless otherwise noted. For RNAi experiments, flies were incubated on standard cornmeal/agar food at 29°C for 5-7 days prior to dissection and imaging to boost Gal4 activity unless otherwise noted.
The following fly strains were used in this study: w¹¹¹⁸, VCP-GFP (Wall et al., 2021 (link)), UAS-VCP-RNAi (Vienna Drosophila Resource Center, #24354; FBst0455418), BamGal4 (Doug Harrison, University of Kentucky, KY, USA), sa-GFP (Chen et al., 2005 (link)), UAS-bam-RNAi [Bloomington Drosophila Stock Center (BDSC), #33631; FBst0033631), UAS-sa-RNAi (BDSC, #36730; FBst0036730), UAS-mia-RNAi (BDSC, #57790; FBst0057790), UAS-Sce-RNAi (BDSC, #67924; FBst0067924), UAS-Ufd1-RNAi (BDSC, #41823; FBst0041823), UAS-CG8042-RNAi (BDSC, #41604; FBst0041604), UAS-Faf2-RNAi (BDSC, #43224; FBst0043224), UAS-casp-RNAi (BDSC, #44027; FBst0044027), UAS-Npl4-RNAi (BDSC, #53004; FBst0053004), UAS-elg1-RNAi (BDSC, #63551; FBst0063551), UAS-Rpt2-RNAi (BDSC, #34795; FBst0034795), Sce^KO (BDSC, #80157; FBst0080157), UAS-GFP^nls (lab stock), VasaGal4; hsFLP.D5, UAS-GFP/cyo; FRT82B, tubGal80/TM6B (Butsch et al., 2022 (link)), EyaGal4 (Leatherman and DiNardo, 2008 (link)), UASp-FRT-H2A-eGFP-PolyA-FRT-H2A-mCherry (Kahney et al., 2021 (link)), tubGal80^AID (McClure et al., 2022 (link)) (BDSC, #92470; FBst0092470) and UAS-VCP^K2A (Chang et al., 2021 (link)).

Butsch T.J., Dubuisson O., Johnson A.E, & Bohnert K.A. (2023). VCP promotes tTAF-target gene expression and spermatocyte differentiation by downregulating mono-ubiquitylated H2A. Development (Cambridge, England), 150(14), dev201557.

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