Detectx serum creatinine kit

Manufactured by Arbor Assays

Sourced in United States

The DetectX® Serum Creatinine kit is a colorimetric assay used to quantitatively measure creatinine levels in serum or plasma samples. The kit employs a simple, direct, and accurate method for the determination of creatinine concentrations.

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Lab products found in correlation

Xbp1000 system, by Kent Scientific (3 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Image pro plus, by Media Cybernetics (1 mentions) Creatinine parameter assay kit, by R&D Systems (1 mentions) Detectx urea nitrogen bun detection kit, by Arbor Assays (1 mentions)

Close spelling variants of this product

Serum creatinine Creatinine

7 protocols using detectx serum creatinine kit

Comprehensive Biomarker Assessment in Animal Model

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Blood pressure (BP) was measured shortly before euthanasia by tail-cuff, using a XBP1000 system (Kent Scientific, Torrington, CT).^{12 (link)} Plasma cholesterol levels were determined at the Mayo Immunochemistry Laboratory using an automatic chemical analyzer with enzymatic assay kits. The level of plasma creatinine was assayed using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI).^{11 (link)} Standards or samples were pipetted into a microtiter plate with an assay diluent, and the color generating reaction was initiated with the DetectX® Creatinine Reagent. Urinary microalbumin was measured by the Mouse Microalbumin ELISA Kit (Kamiya Biomedical Company, Seattle, WA)^{13 (link)} according to the manufacturer’s instructions.

Kim S.R., Jiang K., Ogrodnik M., Chen X., Zhu X.Y., Lohmeier H., Ahmed L., Tang H., Tchkonia T., Hickson L.J., Kirkland J.L, & Lerman L.O. (2019). Increased Renal Cellular Senescence in Murine High-fat Diet: Effect of the Senolytic Drug Quercetin. Translational research : the journal of laboratory and clinical medicine, 213, 112-123.

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Systolic Blood Pressure and Kidney Biomarkers

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Systolic blood pressure (SBP) was measured biweekly by tail-cuff (XBP1000 system Kent Scientific, Torrington, CT) (Cheng et al., 2009 (link)). Plasma creatinine was assayed using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI)(Kim, Jiang, et al., 2019 (link)) and plasma activin-A by a Quantikine ELISA kit (R&D) (Kim, Puranik, et al., 2021 (link)).

Kim S.R., Jiang K., Chen X., Puranik A.S., Zhu X.Y., Lerman A., Tchkonia T., Kirkland J.L, & Lerman L.O. (2022). Selective kidney targeting increases the efficacy of mesenchymal stromal/stem cells for alleviation of murine stenotic-kidney senescence and damage. Journal of tissue engineering and regenerative medicine, 16(6), 550-558.

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Renal Injury Quantification via Histology

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Blood samples were obtained from the inferior vena cava, and both kidneys were harvested after full exsanguination. Serum creatinine concentrations were measured using a DetectX Serum Creatinine Kit (Arbor Assays, Ann Arbor, MI, USA). Kidneys were harvested, washed with saline, fixed with 4% paraformaldehyde, and embedded in paraffin. Renal sections were stained with periodic acid-Schiff (PAS) stain, and CM-related injury, particularly in the proximal tubules and outer medulla, was examined. Ten randomly chosen, non-overlapping fields from the same section of kidney were captured (×400, Olympus BX50, Japan). The injured tubules were traced with the exclusion of Bowman’s capsule, peritubular capillaries and large vessels on each photograph using Image-Pro Plus (Media Cybernetics, Silver Spring, MD, USA). The injured area was expressed as the proportion of the traced field compared to the entire section.

Kim J.E., Bae S.Y., Ahn S.Y., Kwon Y.J, & Ko G.J. (2019). The role of nuclear factor erythroid-2-related factor 2 expression in radiocontrast-induced nephropathy. Scientific Reports, 9, 2608.

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Serum and Urine Biomarkers in Murine Infection

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Blood was collected by cardiac puncture (intraperitoneal infection and survival experiment) or via the vena cava inferior from mice euthanized at defined time points. Serum enzyme levels of pancreatic amylase and alanine aminotransaminase (ALT) were measured using the EuroLyser CCA 180 Vet system (QinLAB Diagnostik) according to standard methods recommended by the International Federation of Clinical Chemistry. The Mouse TREM-1 ELISA Kit (RayBiotech), DetectX® Urea Nitrogen (BUN) Detection Kit (Arbor Assays), Mouse Lipocalin-2 (NGAL) ELISA Kit (RayBiotech) and DetectX® Serum Creatinine Kit (Arbor Assays) were used to measure the respective parameters in serum of mice. Urine of mice was collected from mice euthanized 24, 48 and 72 h p.i. from moribund and uninfected mice. Either spontaneous urine was collected or gentle trans-abdominal pressure was applied onto the bladder and urine was collected using untreated glass capillary tubes. Urinary KIM-1 and creatinine levels were measured using the Mouse TIM-1 ELISA Kit (RayBiotech) and the Creatinine Parameter Assay Kit (R&D Systems), respectively. KIM-1 levels were normalized to urinary creatinine to account for differences in urinary concentration.

Dunker C., Polke M., Schulze-Richter B., Schubert K., Rudolphi S., Gressler A.E., Pawlik T., Prada Salcedo J.P., Niemiec M.J., Slesiona-Künzel S., Swidergall M., Martin R., Dandekar T, & Jacobsen I.D. (2021). Rapid proliferation due to better metabolic adaptation results in full virulence of a filament-deficient Candida albicans strain. Nature Communications, 12, 3899.

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Serum Creatinine Measurement After HI

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Blood samples were collected from the tail 5 weeks after HI (2 weeks after the end of the lithium treatment). Serum was isolated by centrifugation at 2,000×g for 15 min at 20°C and stored at −80°C. The serum creatinine level (mg/dL) was measured using the DetectX@ serum creatinine kit (Arbor Assays, Michigan, US. cat: KBO2-H1) according to the manufacturer's instructions.

Xie C., Zhou K., Wang X., Blomgren K, & Zhu C. (2014). Therapeutic Benefits of Delayed Lithium Administration in the Neonatal Rat after Cerebral Hypoxia-Ischemia. PLoS ONE, 9(9), e107192.

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Telemetry Interference in MRI Experiments

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Telemetry transducers interfere with MRI. Therefore, using a XBP1000 system (Kent Scientific, Torrington, CT), systolic blood pressure (SBP) was measured by tail-cuff at baseline, 2, and 4 weeks. Plasma creatinine level and renin content were evaluated using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI) and the Angiotensin-I RIA kit (ALPCO, Salem, NH), respectively.

Chen X.J., Kim S.R., Jiang K., Ferguson C.M., Tang H., Zhu X., Lerman A., Eirin A, & Lerman L.O. (2021). Renovascular disease induces senescence in renal scattered tubular-like cells and impairs their reparative potency. Hypertension (Dallas, Tex. : 1979), 77(2), 507-518.

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Ischemia-Reperfusion Injury Model in Mice

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The left and right renal arteries of mutant and their littermate control mice (9–10 weeks old, male) were clamped for 30 min using vascular clamps (Fine Science Tools, CA) through a midline abdominal incision under general anesthesia. After ischemia, the clamps were removed, the wounds were sutured, and the animals were allowed to recover for 24 h before euthanization. Blood was collected by cardiac puncture in heparin-containing tube, and creatinine levels were determined using the DetectX serum creatinine kit (Arbor Assays, Ann Arbor, MI). Kidneys were dissected from mice. One kidney of a mouse was fixed in 4% paraformaldehyde, embedded in paraffin, and stained for the Periodic acid-Schiff (PAS) dye and a Ly6B antibody. The other kidney from the same mouse were digested with Collagenase I (300 U) and analyzed by flow cytometry.

Yuan Q., Ren C., Xu W., Petri B., Zhang J., Zhang Y., Kubes P., Wu D, & Tang W. (2017). PKN1 directs polarized RAB21 vesicle trafficking via phosphorylation of RPH3A and is important for neutrophil adhesion and ischemia-reperfusion injury. Cell reports, 19(12), 2586-2597.

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