The sections were deparaffinized in xylene and rehydrated in water. Masson’s trichrome staining kit (DAKO, Cat#AR173) was performed following the manufacturer’s procedure protocol and mounted using Permount toluene solution (Thermo Fisher Scientific). Triplicate images of the stained sections were collected for representative areas of 2560 × 1920 pixels (0.44 μm/pxls) using a Zeiss AX10 light microscope. The positive blue stain was converted to black using Adobe Photoshop CS5’s (version 12.0 × 64) magic wand tool. The images were imported into ImageJ (version 1.46r), and the black selections were quantified as percent area positive for collagen. The images were normalized against the periphery of the duct epithelia in which the final ratio represents collagen/epithelia.
Ax10 light microscope
Manufactured by Zeiss
Sourced in Germany
The AX10 light microscope is a high-quality optical instrument designed for versatile imaging and analysis applications. It features a sturdy construction, precise optics, and a range of configurable components to meet the needs of various research and educational settings. The core function of the AX10 is to provide clear, detailed observations and magnification of microscopic specimens.
Automatically generated - may contain errors
Lab products found in correlation
Masson s trichrome staining kit, by Agilent Technologies (1 mentions)
Permount toluene solution, by Thermo Fisher Scientific (1 mentions)
Senescence β galactosidase staining kit, by Beyotime (1 mentions)
Paraformaldehyde (pfa), by Solarbio (1 mentions)
Axiocam 105 color, by Zeiss (1 mentions)
Axiovision 4, by Zeiss (1 mentions)
Axiocam mrc5, by Zeiss (1 mentions)
6 protocols using ax10 light microscope
1
Quantifying Collagen in Duct Epithelia
2
Histological Analysis of Developmental Tissues
3
Senescence Assessment in HUVECs
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To evaluate whether 3-MCPD induced senescence in HUVECs, SA-β-gal staining was performed using a Senescence β-Galactosidase Staining kit (Beyotime Institute of Biotechnology). Briefly, the cells were fixed using 4% paraformaldehyde (Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 10 min and washed with PBS three times. The cells were then stained using 1 ml β-galactosidase staining working solution at 37°C overnight and washed with PBS. Finally, the cells were assessed and manually quantified using an AX10 light microscope (Carl Zeiss AG; magnification, ×20). Data were presented as SA-β-gal positive cells/cells which represented the ratio of cells stained blue to all cells in each field of view manually.
4
Diversity of Macrofungi in Chinese Forests
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Specimens were collected from Zhejiang Province and Tibet Autonomous Region, respectively. The collection sites in Zhejiang are the subtropical areas with the evergreen broadleaf forests dominated by Lithocarpus brevicaudatus. Meanwhile, the collection sites in Tibet are the plateau-alpine areas with coniferous forests dominated by Abies georgei var. smithii. Fresh basidiomata were photographed in the field. Dried specimens were deposited in the Herbarium of Mycology, Jilin Agricultural University (HMJAU), Changchun, China. Macroscopic characteristics were measured and recorded for every basidiomata and color codes followed Kornerup & Wanscher (1978) . Microscopic features were examined and described in 5% KOH, Congo Red or Melzer’s reagent and observed using a Zeiss AX10 light microscope. Thirty to forty mature basidiospores were measured (excluding apiculus and ornamentation) per collection. Q = variation in the L/W ratios between the specimens studied. Xav. and Qav. = average value of basidiospores of per specimen.
5
Optical Imaging of Soiled Clot Patches
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Optical images of clean, soiled and soiled clot patches washed with 500 Gy assisted biosurfactant at 60 °C under stirring conditions were captured using AX10 Zeiss light microscope coupled with Axiocam 105 color (Germany) at NCRRT. Images representing the fabric threading were captured at 25X and 100X magnification.
6
Polarized Light Microscopy of Crystallites
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The observations were performed using a AX10 Zeiss light microscope (Carl Zeiss, Oberkochen, Germany) under polarized light. The samples were observed in the optical resolution ×400. Pictures were collected using Axiocam MRC5 and images were processed using Axiovision 4.0 software (Carl Zeiss, Oberkochen, Germany). The presence of crystallites was determined by the observation of birefractive entities under polarized light.
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