Anti rabbit hrp conjugated secondary antibody

Fetal bovine serum (FBS), Dulbecco's modified Eagle medium (DMEM), and DMEM : nutrient mixture F-12 (DMEM/F-12) were obtained from Thermo Fisher Scientific (Pittsburgh, USA). CD was purchased from ChemFace (Wuhan, China). 3-[4,5-Dimethylthiazol-2-yl]2,5-diphenyl tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were procured from Duchefa Biochemie (Haarlem, the Netherlands). Rabbit antibodies, E-cadherin, slug, snail, ATG5, beclin-1, and LC3B were purchased from Cell Signaling Technology (Beverly, USA). The anti-mouse HRP-conjugated secondary antibody and anti-rabbit HRP-conjugated secondary antibody were obtained from Enzo Biochem (Farmingdale, USA). Acridine orange and 3MA (3-methyladenine) were obtained from Sigma-Aldrich (St. Louis, USA).

Fetal bovine serum (FBS), minimum essential medium alpha medium (α-MEM), penicillin-streptomycin, and trypsin-EDTA were purchased from GIBCO (Grand Island, NY, USA). Dulbecco's Modified Eagle medium (DMEM) was obtained from Thermo Fisher Scientific (Pittsburgh, PA, USA). Cordycepin, alizarin red S, an Alkaline Phosphatase Assay Kit, and TRAP Staining Kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). RANKL was purchased from R&D systems (Minneapolis, MN, USA). 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyl tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were obtained from DUCHEFA Biochemie (Haarlem, The Netherlands). Mouse antibodies BMP-2, Runx-2, Osterix, Osteopontin, and sRANKL were purchased from Abcam (Cambridge, UK). Antibodies of mouse TRAF6, cFOS, phospho-cFOS, NFATc1, cathepsin K, p38 MAPK, phospho-p38 MAPK, ERK1/2, phospho-ERK1/2, JNK, and phosphor-JNK were provided by Cell Signaling Technology (Beverly, MA, USA). The anti-mouse HRP-conjugated secondary antibody and anti-rabbit HRP-conjugated secondary antibody were purchased from Enzo Biochem (Farmingdale, NY, USA). TOPreal™ qPCR 2X PreMIX and M-MLV cDNA Synthesis Kit were obtained from Enzynomics (Dajeon, KOR). PCR primers (OSCAR, Ctsk1, NFATc1, GADPH) were purchased from Qiagen (Hilden, DEU).