Inflammation markers including TNF-α (ab46070), IL-1β (ab100768), IL-6 (ab100772), IL-10 (ab133112), and ICAM-1 (CD54) (ab100763) ELISA kits were obtained from Abcam Co., Eugene, OR, USA. As for the apoptotic signaling markers, cleaved caspase-3 (KHO1091) was purchased from Thermo Fisher Scientific Inc. Waltham, MA, USA, and caspase-9 (LS-F4141) was acquired from Biocompare, San Francisco, CA, USA. These markers were measured according to the manufacturer’s instructions using a SpectraMax i3X (Molecular devices San Jose, CA, USA) microplate reader.
Cleaved caspase 3 kho1091
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Cleaved caspase-3 (KHO1091) is a laboratory reagent used for the detection of cleaved caspase-3, a key effector of apoptosis. It provides a tool for the quantitative measurement of cleaved caspase-3 levels in cellular samples.
Automatically generated - may contain errors
4 protocols using cleaved caspase 3 kho1091
1
Inflammation and Apoptosis Markers Assay
2
Quantifying Inflammatory and Apoptotic Markers
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Inflammation markers including TNF-α (ab46070), IL-1β (ab100768), IL-6 (ab100772) and IL-10 (ab133112) ELISA kits were obtained from Abcam Co., Eugene, OR, USA. As for the apoptotic signaling markers, cleaved caspase-3 (KHO1091) was purchased from Thermo Fisher Scientific Inc., Waltham, MA, USA, whereas caspase-9 (LS-F4141) was acquired from Biocompare, San Francisco, CA, USA. These markers were measured according to the manufacturer’s instructions using a microplate reader SpectraMax i3X (Molecular devices, San Jose, CA, USA).
3
Inflammation and Apoptosis Signaling ELISA
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Inflammation markers, including TNF-α (ab46070), IL-1β (ab100768), IL-6 (ab100772), and NF-κB (ab176648) ELISA kits were obtained from Abcam Co. (Eugene, OR, USA). As for the apoptotic signaling markers, cleaved caspase-3 (KHO1091) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), whereas Bcl2 (MBS2881713) and Bax (MBS935667) ELISA kits were acquired from My BioSource (San Diego, CA, USA). These markers were measured according to the manufacturer’s instructions using the microplate reader SpectraMax i3X (Molecular devices San Jose, CA, USA).
4
Cleaved Caspase-3 and Caspase-9 Assay
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Cleaved caspase-3 (KHO1091) was obtained from Thermo Fisher Scientific Inc., Waltham, MA, USA, whereas caspase-9 (LS-F4141) was attained from Biocompare, CA, USA.
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