30 plex nhp procartaplex panel

Manufactured by Thermo Fisher Scientific

The 30-Plex NHP ProcartaPlex Panel is a multiplex immunoassay designed to measure the concentrations of 30 different analytes in non-human primate samples. The panel allows for the simultaneous detection and quantification of multiple analytes in a single sample, providing a comprehensive analysis of the target analytes.

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Lab products found in correlation

Bio plex manager software v 6, by Bio-Rad (2 mentions) Bio plex 200 system, by Bio-Rad (2 mentions)

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ProcartaPlex panels (2 citations)

2 protocols using 30 plex nhp procartaplex panel

Multiplex Cytokine and Chemokine Profiling

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To analyze peripheral soluble cytokines and chemokines, a luminex assay was performed with Cytokine & Chemokine 30-Plex NHP ProcartaPlex Panel (Invitrogen, EPX300-40044-901). The analytes in this panel are BLC (CXCL13); Eotaxin (CCL11); G-CSF (CSF-3); GM-CSF; IFN alpha; IFN gamma; IL-1 beta; IL-10; IL-12p70; IL-13; IL-15; IL-17A (CTLA-8); IL-18; IL-1RA; IL-2; IL-23; IL-4; IL-5; IL-6; IL-7; IL-8 (CXCL8); IP-10 (CXCL10); I-TAC (CXCL11); MCP-1 (CCL2); MIG (CXCL9); MIP-1 alpha (CCL3); MIP-1 beta (CCL4); sCD40L; SDF-1 alpha (CXCL12a); TNF alpha. Plasma was thawed on ice and manufacturers instructions were followed to prepare a 96-well plate with samples performed in duplicates and read on a Bio-Plex 200 System (Bio-Rad Laboratories, Hercules, CA). Results were calculated using Bio-Plex Manager Software v6.2 (Bio-Rad) and the mean concentration of each analyte was plotted.

Moström M.J., Yu S., Tran D., Saccoccio F.M., Versoza C.J., Malouli D., Mirza A., Valencia S., Gilbert M., Blair R.V., Hansen S., Barry P., Früh K., Jensen J.D., Pfeifer S.P., Kowalik T.F., Permar S.R, & Kaur A. (2023). Protective effect of pre-existing natural immunity in a nonhuman primate reinfection model of congenital cytomegalovirus infection. PLOS Pathogens, 19(10), e1011646.

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Cytokine and Chemokine Profiling Using Luminex

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To analyze peripheral soluble cytokines and chemokines, a luminex assay was performed with Cytokine & Chemokine 30-Plex NHP ProcartaPlex^™ Panel (Invitrogen, EPX300–40044-901). The analytes in this panel are BLC (CXCL13); Eotaxin (CCL11); G-CSF (CSF-3); GM-CSF; IFN alpha; IFN gamma; IL-1 beta; IL-10; IL-12p70; IL-13; IL-15; IL-17A (CTLA-8); IL-18; IL-1RA; IL-2; IL-23; IL-4; IL-5; IL-6; IL-7; IL-8 (CXCL8); IP-10 (CXCL10); I-TAC (CXCL11); MCP-1 (CCL2); MIG (CXCL9); MIP-1 alpha (CCL3); MIP-1 beta (CCL4); sCD40L; SDF-1 alpha (CXCL12a); TNF alpha. Plasma was thawed on ice and manufacturers instructions were followed to prepare a 96-well plate with samples performed in duplicates and read on a Bio-Plex^® 200 System (Bio-Rad Laboratories, Hercules, CA). Results were calculated using Bio-Plex Manager^™ Software v6.2 (Bio-Rad) and the mean concentration of each analyte was plotted.

Moström M., Yu S., Tran D., Saccoccio F., Versoza C.J., Malouli D., Mirza A., Valencia S., Gilbert M., Blair R., Hansen S., Barry P., Früh K., Jensen J.D., Pfeifer S.P., Kowalik T.F., Permar S.R, & Kaur A. (2023). Protective effect of pre-existing natural immunity in a nonhuman primate reinfection model of congenital cytomegalovirus infection. bioRxiv.

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