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Ab112

Manufactured by Beyotime
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The AB112 is a precision laboratory balance designed for accurate weighing of small samples. It features a high-resolution digital display and a durable stainless-steel weighing platform. The AB112 provides reliable and consistent measurements within its specified capacity and readability range.

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Lab products found in correlation

Ab51603, by Abcam (2 mentions) Ab26350, by Abcam (1 mentions) Ab126, by Beyotime (1 mentions) A23420, by Abbkine (1 mentions) A23210, by Abbkine (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) β actin, by ZSGB-BIO (1 mentions) Bond max automated ihc ish stainer, by Leica (1 mentions) Ecl chemiluminescence kit, by Beyotime (1 mentions) Bca kit, by Beyotime (1 mentions) Af0057, by Beyotime (1 mentions)

4 protocols using ab112

1

Antibodies for FEN1, γH2AX, and Apoptosis

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Antibodies used in this paper are listed as following: anti‐FEN1 antibody (42 282, Genetex), anti‐γH2AX antibody (ab26350, Abcam), anti‐GAPDH antibody (264 140, Abmart), anti‐BAX antibody (AB026, Beyotime), anti‐BCL‐XL antibody (AB126, Beyotime), anti‐BCL‐2 antibody (AB112, Beyotime), Dy Light 594 Goat‐anti Rabbit (A23420, Abbkine), Dy Light 488 Goat‐anti mouse (A23210, Abbkine).
Li J., Wang J., Chang H., Deng S., Du J., Wang X., Hu H., Li D., Xu X., Guo W., Song Y., Guo Z., Sun M., Wu Y, & Liu S. (2019). FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells. Cancer Medicine, 8(18), 7774-7780.
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2

Immunoblotting for Apoptosis Regulators

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Equal amounts of total cell lysates were separated by SDS-PAGE and electrophoretically transferred to PVDF membranes. The membrane was blocked with 5% skimmed milk and incubated with primary antibodies against DYNLL1 (ab51603, 1:1000; Abcam), BIM (#12933, 1:1000; Cell Signaling Technology), BCL2 (AB112, 1:1000; Beyotime Biotechnology), β‐actin (1E9A3, 1:1000; ZSGB‐BIO), and FLAG (#14793, 1:1000, Cell Signaling Technology).
Li Q., Zhang Z., Jiang H., Hou J., Chai Y., Nan H., Li F, & Wang L. (2022). DLEU1 promotes cell survival by preventing DYNLL1 degradation in esophageal squamous cell carcinoma. Journal of Translational Medicine, 20, 245.
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3

Immunohistochemical Analysis of DYNLL1 and BCL2

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Immunohistochemical staining of DYNLL1 and BCL2 was performed using primary antibodies against DYNLL1 (ab51603, 1:600; Abcam) and BCL2 (AB112, 1:200; Beyotime Biotechnology) on tissue microarray sections by a BOND-MAX Automated IHC/ISH Stainer (Leica). The immunostaining degree of the DYNLL1 and BCL2 proteins was evaluated as previously described [20 (link)] by pathologists based on the nuclear staining intensity (intensity score) and percentage of positive cells (extent score). The final immunoreactivity score for each sample was the product of the intensity score and extent score.
Li Q., Zhang Z., Jiang H., Hou J., Chai Y., Nan H., Li F, & Wang L. (2022). DLEU1 promotes cell survival by preventing DYNLL1 degradation in esophageal squamous cell carcinoma. Journal of Translational Medicine, 20, 245.
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4

Protein Expression Analysis in Cells

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The cells were lysed with RIPA (Beyotime, P0013B) containing 1% PMSF (Beyotime, ST505). The protein concentrations were determined using BCA kit (Beyotime, P0010S) according to the instructions, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) at 20 mg/well. After the electrophoresis, the proteins were transferred onto the NC membrane, and rabbit NSUN2 (Proteintech, 20854-1-AP, 1:200), Nrf2 (Proteintech, 16396-1-AP, 1:200), Bax (Beyotime, AF0057, 1:500), Bcl-2 (Beyotime, AB112, 1:500), HO-1 (Beyotime, AF1333, 1:500), NQO1 (Beyotime, AF7614, 1:500), and α-Tubulin Rabbit polyclonal antibody (Beyotime, AF5012, 1:500) were incubated overnight at 4 °C. Then Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG (H + L) (Beyotime, A0208, 1:2000) was added and incubated at room temperature for 1 h. Finally, the concentration of the proteins was measured using the enhanced chemiluminescence (ECL) chemiluminescence kit (Beyotime, P0018).
Wang Y., Zan Y., Huang Y., Peng X., Ma S., Ren J., Li X., Wei L., Wang X., Yuan Y., Tang J., Zhan Z., Wang Z, & Ding Y. (2023). NSUN2 alleviates doxorubicin-induced myocardial injury through Nrf2-mediated antioxidant stress. Cell Death Discovery, 9, 43.
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