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Phospho bad ser112

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Phospho-Bad (Ser112) is a lab equipment product that detects and measures the phosphorylation of the pro-apoptotic protein Bad at the serine 112 residue. It is used to study cell signaling pathways and apoptosis regulation.

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Lab products found in correlation

Caspase 8, by Cell Signaling Technology (2 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Caspase 9, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Cytochrome c, by Cell Signaling Technology (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) Phospho erk, by Cell Signaling Technology (2 mentions) Phospho jnk, by Cell Signaling Technology (2 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions) Wy14643, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Doxorubicin, by Merck Group (1 mentions) Annexin 5 fitc apoptosis detection kit, by Merck Group (1 mentions) Phospho bad ser136, by Cell Signaling Technology (1 mentions) Phospho foxo 3a ser253, by Cell Signaling Technology (1 mentions) Phospho mk2 thr334, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg hrp linked secondary antibody, by Cell Signaling Technology (1 mentions) Vinculin, by Santa Cruz Biotechnology (1 mentions) Irdye 680rd donkey anti mouse igg, by LI COR (1 mentions)

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Phospho-Bad (10 citations)

5 protocols using phospho bad ser112

1

Apoptosis Signaling Pathway Analysis

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Doxorubicin, Cycloheximide (CHX), MG132, WY-14643, and Annexin V-FITC Apoptosis Detection Kit were purchased from Sigma-Aldrich (MA, USA). Antibodies against Flag, Caspase-3, Caspase-8, Caspase-9, PARP, Bax, phospho-Bad (Ser112), Cytochrome C, p53, p53(Ser15), p53(Ser20), p73, and GAPDH were purchased from Cell Signaling Technology (Boston, USA). Antibodies against ACOX1 was purchased from Abcam (Cambridge, U.K.). Antibody against puromycine was purchased from Merk (Darmstadt, Germany). LEHD, DEVD, and IETD were purchased from R&D Systems (MN, USA). Raji and Daudi cells were obtained from the ATCC (Manassas, VA, USA) and were cultured in RPMI-1640 (Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, USA). Cells were maintained at 37°C in a humidified 5% CO2 atmosphere.
Zheng F.M., Chen W.B., Qin T., Lv L.N., Feng B., Lu Y.L., Li Z.Q., Wang X.C., Tao L.J., Li H.W, & Li S.Y. (2019). ACOX1 destabilizes p73 to suppress intrinsic apoptosis pathway and regulates sensitivity to doxorubicin in lymphoma cells. BMB Reports, 52(9), 566-571.
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2

Protein Expression Analysis by Western Blot

Cited 1 time
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Identical levels of proteins were separated by SDS-PAGE and analyzed by western-blot with the indicated antibodies as described previously [20 (link), 21 (link)]. Antibodies used for western blot: cleaved-PARP, cleaved-caspase 7, cleaved-caspase 8, cleaved-caspase 9, BIM, phospho-JNK, phospho-AKT, phospho-BAD-ser136, phospho-Foxo-3a-ser253, phospho-ERK, phospho-BAD-ser112, (Cell Signaling), tubulin(SIGMA), and Actin (Millipore). Quantifications were performed with ImageJ software.
Kowalski-Chauvel A., Gouaze-Andersson V., Vignolle-Vidoni A., Delmas C., Toulas C., Cohen-Jonathan-Moyal E, & Seva C. (2017). Targeting progastrin enhances radiosensitization of colorectal cancer cells. Oncotarget, 8(35), 58587-58600.
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3

Immunoblot Analysis of Signaling Pathways

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Primary antibodies from Cell Signaling Technology (Danvers, MA, USA) were used to detect Caspase 3 (#9662; 1:1000/1:500 [full-length/cleaved]), Bad (#9239; 1:500), phospho-Bad (Ser112) (#5284; 1:500), Erk (#4695; 1:1000), phospho-Erk (#9101; 1:1000), Akt (#9272; 1:1000), phospho-Akt (#4060; 1:200), MK2 (#3042; 1:500), phospho-MK2 (Thr222) (#3316; 1:200), phospho-MK2 (Thr334) (#3007; 1:200), p38 (#9212; 1:1000), phospho-p38 (#4511; 1:2000), JNK (#9252; 1:1000), phospho-JNK (#9251; 1:500), c-Jun (#9165; 1:1000) and phospho-c-Jun (#3270; 1:1000). Primary antibodies against p53 (sc-47698; 1:1000), phospho-p53 (Ser392) (sc-51690; 1:500) and Vinculin (sc-73614; 1:1000) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Polyclonal antibodies against TCRV (1:1000) and JUNV (1:1000) NP (produced in guinea pigs [15 (link)]) were used to confirm viral infection. Fluorescently labeled secondary antibodies were purchased from LI-COR (IRDye 680RD Donkey anti-Mouse IgG, IRDye 680RD Donkey anti-Guinea Pig IgG, IRDye 800CW Donkey anti-Rabbit IgG) and used at a dilution of 1:15000. A goat anti-rabbit IgG HRP-linked secondary antibody (#7074; 1:5000, Cell Signaling Technology) was used for the detection of Bad.
Holzerland J., Fénéant L, & Groseth A. (2022). Regulation of Stress-Activated Kinases in Response to Tacaribe Virus Infection and Its Implications for Viral Replication. Viruses, 14(9), 2018.
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4

Immunoblotting Analysis of Apoptosis Regulators

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Protein lysates extracted from cells were used to detect protein expression levels of caspase 3 (Cell Signaling, Stillorgan, Ireland; #9662), caspase 8 (Cell Signalling; #9746), FADD (Cell Signalling; #2782), caspase 9 (Cell Signalling; #9508), Apaf‐1 (Cell Signalling; #8723), Bcl‐2 (Cell Signalling; #4223), Bax (Cell Signalling; #5023), Bak (Cell Signalling; #3814), Bad (Cell Signalling; #9292), Phospho‐Bad (Ser112) (Cell Signalling; #9291), Phospho‐p53 (Ser15) (Cell Signalling; #9284), p53 (Cell Signalling; #2524), cytochrome c (Cell Signalling; #4272), COX IV (Cell Signalling; #4844), β‐Tubulin (Cell Signalling; #2146) and GAPDH (Cell Signalling; #5174). The immunoblotting protocol was previously described.19
Long Q., Li L., Wang H., Li M., Wang L., Zhou M., Su Q., Chen T, & Wu Y. (2018). Novel peptide dermaseptin‐PS1 exhibits anticancer activity via induction of intrinsic apoptosis signalling. Journal of Cellular and Molecular Medicine, 23(2), 1300-1312.
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5

Antibody Identification Procedure

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Rabbit anti-actin and mouse anti-α-tubulin were from Sigma (St. Louis, MO); rabbit antibodies to caspase-3, Bcl2, Bcl-xL, Bad, phospho-Bad (ser-112) and Bax were from Cell signaling (Danvers, MA, USA); rabbit antibodies to phospho-Bim (ser-69), Bim, Puma, phospho-SAPK/JNK (Thr-183/Tyr-185), SAPK/JNK were from Cell signaling. Secondary horseradish peroxidase (HRP) conjugated anti-rabbit IgG (from donkey) and HRP conjugated anti-mouse IgG (from sheep) were from GE healthcare (Nyon, Switzerland).
Patel D., Ythier D., Brozzi F., Eizirik D.L, & Thorens B. (2015). Clic4, a novel protein that sensitizes β-cells to apoptosis. Molecular Metabolism, 4(4), 253-264.
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