Bmscs

Manufactured by Thermo Fisher Scientific

Sourced in United States, China

BMSCs are multipotent stromal cells derived from bone marrow. They have the ability to differentiate into various cell types, including osteoblasts, chondrocytes, and adipocytes. BMSCs are commonly used in regenerative medicine and tissue engineering research.

Automatically generated - may contain errors

Lab products found in correlation

23 protocols using bmscs

Lentiviral Transfection of BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bone marrow mesenchymal stem cells (BMSCs) were purchased from Bio-world (Shanghai, China). BMSCs were suspended in a medium containing 10% FBS and 100 U/ml penicillin, and 100 mg/ml streptomycin, then cultured in 37 °C with 5% CO₂.
The lentivirus of miR-27a mimics, miR-27a inhibitor, and negative control (NC) were purchased from Gemma (Shanghai, China). Lentivirus was transfected into BMSCs using Lipofectamine® 3000 reagent according to the protocols (Invitrogen, MA, USA).

Cui Y., Huang T., Zhang Z., Yang Z., Hao F., Yuan T, & Zhou Z. (2022). The potential effect of BMSCs with miR‐27a in improving steroid-induced osteonecrosis of the femoral head. Scientific Reports, 12, 21051.

+ Open protocol

+ Expand

Osteogenic Differentiation of Human BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bone mesenchymal stem cells (BMSCs; passage 2) were available from Cell Bank of the Chinese Academy of Sciences (China). BMSCs were grown in α-Minimum Essential Medium (MEM) (Invitrogen, Thermo Fisher Scientific, USA) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37°C in 5% carbon dioxide (CO₂). For osteogenic differentiation induction, cells planted in a 12-well plate (5 × 10⁵ cells/well) were treated for 14 days with 10 mM of β-glycerophosphate, 200 μM of ascorbic acid, and 100 nM of dexamethasone (all from MilliporeSigma, USA). The medium was changed every three days, and cells were tested at zero, seven, and 14 days.

Xin W., Yuan S., Wang B., Qian Q, & Chen Y. (2021). Hsa_circ_0066523 promotes the proliferation and osteogenic differentiation of bone mesenchymal stem cells by repressing PTEN. Bone & Joint Research, 10(8), 526-535.

+ Open protocol

+ Expand

Cell Culture Protocol for HEK293T, BMSCs, HUVECs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human embryonic kidney 293T (HEK293T) and human BMSCs were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). BMSCs and HEK293T cells were cultured in α minimum essential medium and Dulbecco's modified Eagle's medium (Invitrogen). Human umbilical vein endothelial cells (HUVECs) were purchased from Procell (Wuhan, China), and cultured in endothelial cell medium (ECM, ScienCell).

Yu H., Wang K., Liu P., Luo P., Zhu D., Yin J., Yang Q., Huang Y., Gao J., Ai Z., Chen Y, & Gao Y. (2021). miR‐4286 functions in osteogenesis and angiogenesis via targeting histone deacetylase 3 and alleviates alcohol‐induced bone loss in mice. Cell Proliferation, 54(6), e13054.

+ Open protocol

+ Expand

Characterization of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 3rd generation BMSCs (CinoAsia Co., Ltd.) were digested using trypsin, after which 1x10⁵/ml cell suspensions in DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc.) were generated in an eppendorf tube. A total of 10 µl fluorescently-labelled CD34 (cat. no. ab8158), CD45 (cat. no. ab33923) and CD44 (cat. no. ab119348) antibodies (all, Abcam; all, 1:100) were added to 100 µl of the cell suspension and incubated for 1 h at 37˚C. Flow cytometry was performed to detect individual cell markers (FACSMelody; Becton, Dickinson and Company; software, Flowjo, version 7.6; Becton, Dickinson and Company). BMSCs were counted and resuspended in complete DMEM/F12 supplemented with 10% fetal bovine serum and 1% senicillin-streptomycin solution (all, Gibco; Thermo Fisher Scientific, Inc.). Samples were cultured at 37˚C and cell density was adjusted to 1x10⁶ cells/ml.

Huang X., Chen Z., Zhao G., Shi J., Huang G., Chen F., Wei Y., Xia J., Chen J, & Wang S. (2020). Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds. Experimental and Therapeutic Medicine, 20(5), 19.

+ Open protocol

+ Expand

Labeling Mouse Bone Marrow Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMSCs (Cyagen, USA) derived from Balb/c mice were cultured in complete DMEM/low-glucose medium (HyClone, USA) containing 10% fetal bovine serum (Gibco, USA) and 1% 100 U/ml Penicillin-Streptomycin (Gibco). In BMSCs of P2 generation with a degree of integration up to 80% at the logarithmic growth stage, they were labeled by Cell Tracker TM CM-Dil (MKbio, China) according to the manufacturer's instructions. Specifically, CM-Dil was dissolved in 1 mg/ml dimethyl sulfoxide as a working solution and added to the BMSCs (3 ml of working solution per Petri dish). Subsequently, cells were incubated at 37 °C for 3 min and then incubated for another 15 min at 4 °C. Light should be avoided during labeling. Finally, BMSCs were washed with phosphate-buffered solution (PBS) and incubated with complete DMEM/low-glucose medium (Thermo Fisher Scientific, MA, USA). The culture medium was replaced once a day, and the subculture was used when the degree of fusion reached 80%–90%. BMSCs of the fifth–sixth generations were used for further utilization (16 (link)).

Wang X., Zhao J., Wang X., Zhang J., Wang Y., Wang X., Jia S., Shi N., Lu M., Su H., Zhang J, & Jiang D. (2023). Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway. Frontiers in Surgery, 9, 1027067.

+ Open protocol

+ Expand

Generating Conditioned Media from BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bone marrow-derived mesenchymal stem cells (BMSCs; Thermo Fisher Scientific, Waltham, Massachusetts, USA) at passage 3 to 5 were employed for all experimentation. Bone marrow-derived mesenchymal stem cells at 70% to 80% confluency were treated with serum-free Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with recombinant human TGF-β1 (10 ng/mL; Life Technologies, Grand Island, New York, USA) following 12-hour serum starvation (DMEM+0.1% Bovine Serum Albumin). After 48 hours, the media was collected, centrifuged at 1000 rpm for 5 minutes, and filtered through a .22 μm syringe filter. This CM was employed immediately for all experimentation.

Hiwatashi N., Bing R., Kraja I, & Branski R.C. (2017). Stem Cell–Mediated Paracrine Signaling Alters Fibroplasia in Human Vocal Fold Fibroblasts in Vitro. The Annals of otology, rhinology, and laryngology, 126(8), 581-588.

+ Open protocol

+ Expand

Culturing MHCC97-H and BMSCs for Liver Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

MHCC97-H were provided by the Institute of Liver Cancer at Fudan University. MHCC97-H cells were incubated with 10% FBS with 1X DMEM medium at 37˚C in a 5% CO₂ incubator. Cells were subcultured by washing with PBS, followed by dissociation with 0.25% trypsin and 0.02% EDTA. BMSCs were provided by Saiye Biotechnology Co., Ltd., (cat. no. HUXMA-90011). BMSCs were derived from the bone marrow of healthy adults (18-45 years), purchased from Saiye Biotechnology Co., Ltd., (cat. no. HUXMA-90011). BMSCs were cultured and passaged to the 2nd generation in vitro and then stored frozen. BMSCs activity was tested before each experiment, include BMSCs growth state, differentiation ability and cluster differentiation tests. BMSCs were incubated with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) + 1X MSCM (Beijing Yuhengfeng Technology Co., Ltd.) + L-Glutamine medium at 37˚C in a 5% CO₂ incubator. When cells confluence reached 90%, BMSCs were passaged by washing with PBS, followed by dissociation with 0.25% Trypsin and 0.02% EDTA.

Zhang B., Shang L., Zhang Y., Li T, & Fang Y. (2020). The effect of bone marrow mesenchymal stem cells on highly metastatic MHCC97-H hepatocellular carcinoma cells following OPN and TGFβ1 gene silencing. Experimental and Therapeutic Medicine, 20(4), 3633-3642.

+ Open protocol

+ Expand

Osteogenic and Adipogenic Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bone marrow-derived mesenchymal stromal cells (BMSCs, Lonza, Basel, Switzerland) were cultured as previously described [25 (link)]. Briefly, BMSCs were maintained in alpha minimum essential medium (α-MEM, Gibco, Paisley, United Kingdom) supplemented with 10% heat-inactivated fetal calf serum. Following two days of attachment, osteogenic induction was initiated using 100 nM dexamethasone and 10 mM β-glycerophosphate. For adipogenic induction, BMSCs were treated with 0.1 μM dexamethasone, 60 μM indomethacin, and 0.5 mM 3-isobutyl-1-methylxanthine. Cells at passage 7 were used in all experiments and media was refreshed every 3 or 4 days. To block Activin A activity, SB431542 (Sigma-Aldrich, Zwijndrecht, the Netherlands) or Activin A neutralizing antibody (R&D Systems, Minneapolis, Minnesota, United States) was added during cell refreshment.

Zhang S., van de Peppel J., Koedam M., van Leeuwen J.P, & van der Eerden B.C. (2023). HSPB7 oppositely regulates human mesenchymal stromal cell-derived osteogenesis and adipogenesis. Stem Cell Research & Therapy, 14, 126.

+ Open protocol

+ Expand

Culturing Breast Cells and Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human normal breast cells MCF-10A, human breast cancer cell line MDA-MB-231 cells, and human bone marrow mesenchymal stem cells (BMSCs) were purchased from the American Type Culture Collection (ATCC) cell bank (Manassas, VA, USA). The MCF-10A cells were cultured in DMEM/F12 medium (Gibco) containing 2.5 mM glutamine, 20 ng/ml epidermal growth factor, 0.01 mg/ml insulin, 500 ng/ml hydrocortisone, and 5% horse serum in DMEM/F12 medium (Gibco). The MDA-MB-231 cells were cultured in RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (FBS) and 0.5% penicillin-streptomycin. The BMSCs were cultured in DMEM medium (Gibco) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin in DMEM medium (Gibco). All cells were routinely cultured at 37°C in a 5% CO₂ incubator.

Sun H., Dai J., Chen M., Chen Q., Xie Q., Zhang W., Li G, & Yan M. (2022). miR-139-5p Was Identified as Biomarker of Different Molecular Subtypes of Breast Carcinoma. Frontiers in Oncology, 12, 857714.

+ Open protocol

+ Expand

Culturing Hepatoblastoma and Liver Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hepatoblastoma cell lines (huH6 and HepG2), normal liver cells (WRL68), and human bone marrow mesenchymal stem cells (BMSCs) were bought from ATCC (Manassas, VA, USA). Hepatoblastoma cells and WRL68 cells were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% FBS and 1% streptomycin and penicillin (Thermo Fisher Scientific) in an incubator (37°C, 5% CO₂). BMSCs were maintained in α-MEM (Gibco) with 10% FBS (Corning) and 1% streptomycin and penicillin. The cells were maintained under the following conditions: 5% CO₂ and 95% humidity.

Hu Y., Zai H., Jiang W., Yao Y., Ou Z, & Zhu Q. (2021). miR-126 in Extracellular Vesicles Derived from Hepatoblastoma Cells Promotes the Tumorigenesis of Hepatoblastoma through Inducing the Differentiation of BMSCs into Cancer Stem Cells. Journal of Immunology Research, 2021, 6744715.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!