3h methylspiperone

[³H]Methylspiperone (85.5 Ci/mmol), [³H]SCH23390 (73.1 Ci/mmol), [³H]cAMP (25 Ci/mmol) and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA). Tetracycline, hygromycin, blasticidin, DA, (+)-butaclamol, fluphenazine, phenylmethylsulfonyl fluoride (PMSF), GDP, GTPγS, cAMP, isobutylmethylxanthine and ascorbic acid were obtained from Sigma-Aldrich (St. Louis, MO). G418 was obtained from Gemini Bio-Products (West Sacramento, CA), while cell culture reagents were obtained from Invitrogen (Carlsbad, CA). (−)-L-SPD was generously provided by Dr. David Y.W. Lee from the Bio-Organic and Natural Products Laboratory at McLean Hospital (Harvard Medical School, Belmont, MA).

[³H]Methyl spiperone (85.5 Ci/mmol), [³H]SCH23390 (73.1 Ci/mmol), and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA). Tetracycline, hygromycin, blasticidin, dopamine (DA), (+) butaclamol, fluphenazine, phenylmethylsulfonyl fluoride (PMSF), GDP, GTPγS, and ascorbic acid were obtained from Sigma-Aldrich (St. Louis, MO). G418 was obtained from Gemini Bio-Products (West Sacramento, CA); cell culture reagents were obtained from Invitrogen (Carlsbad, CA); and protease inhibitor cocktail, phosphatase inhibitor cocktail, and lysis buffer were obtained from Thermo Scientific (Rockford, IL). All test molecules (Table 1) were either synthesized in-house as described below or purchased from vendors. Asinex Corp. (Winston-Salem, NC): SK603, SK609, SK610, SK611; Enamine, Ltd. (Kiev, Ukraine): Compounds described in Lichtenberger patent²⁸ L1 and L2; Asiba Pharmatech, Inc. (Milltown, NJ): R- and S-enantiomers of SK609. DA and D2R/D3R agonists PD128907 and pramipexole were purchased from Tocris Bioscience (Bio-Techne, Inc., CA).