Lactobacillus isolates were tested for hydrogen peroxide production (H 2 O 2 ) by the qualitative plate method, using horseradish peroxide incorporated in 3,3',5,5'-tetramethylbenzidine (TMB, Sigma) MRS agar medium (Eschenbach et al. 1989 ). Lactobacilli were cultured on MRS agar containing 1mM TMB and 2U/ml horseradish peroxide (Sigma). Plates were incubated in anaerobic conditions at 37 o C for 48 h. Colonies of H 2 O 2 -producing strains turned blue after exposure to air for 30 min.
Horseradish peroxide
Manufactured by Merck Group
Sourced in China, United States
Horseradish peroxidase is an enzyme that catalyzes the oxidation of various organic and inorganic compounds using hydrogen peroxide as the oxidizing agent. It is commonly used as a label in immunoassays, blotting techniques, and histochemical staining procedures.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
2 4 amidinophenyl 6 indolecarbamidine dihydrochloride dapi, by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Abcam (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Rabbit anti p63, by Abcam (1 mentions)
Rhodamine phalloidin, by Merck Group (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Iron 3 sulfate, by Merck Group (1 mentions)
Dichlorodihydrofluorescein diacetate assay dcfh da, by Merck Group (1 mentions)
Thiobarbituric acid tba, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
3 protocols using horseradish peroxide
1
Qualitative Assay for H2O2 Production in Lactobacillus
2
Biocompatible Silkworm Cocoon-Based Materials
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BC bulks were obtained from Hai Nan Yeguo Foods Co., Ltd. (Haikou, China). Silkworm cocoons from B. mori were purchased from Tongxiang City, Zhejiang Province, China. Horseradish peroxide was purchased from Sigma Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (USA). Calcein acetoxymethylester/ Propidium iodide (Calcein AM/PI) were purchased from Yeasen Biotech Co., Ltd. (Shanghai, China). Rhodamine phalloidin, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (USA). Rabbit anti-Ki67, rabbit anti-p63 and fluorescein isothiocyanate (FITC) were purchased from Abcam (UK). All other chemicals were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), were analytical grade and used as received.
3
Antioxidant Evaluation of Fibroblast Cells
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All chemicals have an analytical purity and were used without further purification. Iron(III) chloride (FeCl3·6H2O), iron(III) sulfate (FeSO4), trolox, H2O2, horse radish peroxide, phosphate buffer pH 7, Folin-Ciocalteu reagent, DPPH (2,2-diphenyl-1-picrylhydrazyl), 2,4,6-tripyridyl-s-triazine (TPTZ), trichloroacetic acid (TCA), thiobarbituric acid (TBA), sodium dodecyl sulphate (DSD) and dichlorodihydrofluorescein diacetate assay (DCFH-DA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ethanol and methanol were bought from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM) containing glucose at 4.5 mg/mL (25 mM), penicillin, streptomycin, trypsin–EDTA, fetal bovine serum (FBS) and phosphate buffered saline (PBS) without Ca and Mg were purchased from Gibco (San Diego, USA). DTNB (dithiobis-2-nitrobenzoic acid, Ellman's reagent) was provided by Serva Electrophoresis GmbH (Heidelberg, Germany). The fibroblasts cell line was obtained from American Type Culture Collection (ATCC).
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