180k oligonucleotide microarray

Manufactured by Agilent Technologies

The 180K oligonucleotide microarray is a high-density DNA microarray product offered by Agilent Technologies. It features a collection of 180,000 unique oligonucleotide probes designed for genomic analysis applications.

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Oligonucleotides (6 citations) Oligonucleotide microarrays (2 citations)

3 protocols using 180k oligonucleotide microarray

CGH for Clinically Relevant Aneuploidy

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To detect aneuploidy, array comparative genomic hybridization was performed using a custom 180 K oligonucleotide microarray (Agilent, Santa Clara, CA), with a genome-wide functional resolution of approximately 100 kilobases. Deletions larger than 200 kilobases and duplications larger than 500 kilobases were considered clinically relevant.

Birker K., Ge S., Kirkland N.J., Theis J.L., Marchant J., Fogarty Z.C., Missinato M.A., Kalvakuri S., Grossfeld P., Engler A.J., Ocorr K., Nelson T.J., Colas A.R., Olson T.M., Vogler G, & Bodmer R. (2023). Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrity. eLife, 12, e83385.

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Array CGH for Copy Number Variants

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Array comparative genomic hybridization using a custom 180K oligonucleotide microarray (Agilent, Santa Clara, CA) was performed on a DNA sample from the affected patient with a genome‐wide functional resolution of 100 kilobases (kb). Deletions ≥200 kb and duplications ≥500 kb were considered clinically relevant. Those below the size threshold were considered relevant if sufficient evidence supporting pathogenicity from Online Mendelian Inheritance in Man (OMIM) (http://ncbi.nlm.nih.gov/omim), PubMed (http://ncbi.nlm.nih.gov/pubmed), or ClinGen Dosage Sensitivity Map (http://ncbi.nlm.nih.gov/projects/dbvar/clingen/index.shtml) was present.

Long P.A., Larsen B.T., Evans J.M, & Olson T.M. (2015). Exome Sequencing Identifies Pathogenic and Modifier Mutations in a Child With Sporadic Dilated Cardiomyopathy. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 4(12), e002443.

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Array CGH for Aneuploidy Detection

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To detect aneuploidy, array comparative genomic hybridization was performed using a custom 180K oligonucleotide microarray (Agilent, Santa Clara, CA), with a genome-wide functional resolution of approximately 100 kilobases. Deletions larger than 200 kilobases and duplications larger than 500 kilobases were considered clinically relevant.

Theis J.L., Vogler G., Missinato M.A., Li X., Nielsen T., Zeng X.X., Martinez-Fernandez A., Walls S.M., Kervadec A., Kezos J.N., Birker K., Evans J.M., O'Byrne M.M., Fogarty Z.C., Terzic A., Grossfeld P., Ocorr K., Nelson T.J., Olson T.M., Colas A.R, & Bodmer R. (2020). Patient-specific genomics and cross-species functional analysis implicate LRP2 in hypoplastic left heart syndrome. eLife, 9, e59554.

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