Silybin capsule was obtained from Tianjin Tasly Pharmaceutic Co., Ltd. Total cholesterol (TC) kit, triglycerides assay (TG) kit, low density lipoprotein cholesterol (LDL-C) kit, high-density lipoprotein cholesterol (LDL-C) kit, aspartate aminotransferase (AST) kit, alanine aminotransferase (ALT) kit, and glucose (Glu) kit were obtained from Biosino Bio-Technology and Science Incorporation. Free fatty acid (FFA) kit, superoxide dismutase (SOD) kit, and malondialdehyde (MDA) kit were obtained from Nanjing Jiancheng Bioengineering Institute. Rat insulin kit was purchased from MERCODIA. Rat tumor necrosis factor (TNF-α) kit, rat leptin (LEP) kit, rat interleukin-6 (IL-6) kit, and rat chemokine-1 (MCP-1) kit were obtained from RapidBio Lab.
Rat insulin kit
Manufactured by Mercodia
Sourced in Sweden
The Rat Insulin Kit is a laboratory equipment used to measure insulin levels in rat samples. The kit includes the necessary reagents and materials to perform the analysis. It provides a quantitative method for the determination of insulin in rat serum, plasma, or other biological samples.
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Lab products found in correlation
7 protocols using rat insulin kit
1
Silybin Regulates Lipid Metabolism and Inflammation
2
Insulin Secretion in INS-1 Cells
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After a 24 h culture of the INS-1 cells, the medium was replaced by 20 mM high glucose RPMI 1640 medium. The cells were treated with FL at 50, 100, and 200 μg/ml concentrations in absence or presence of 0.75 mM MET for 2 days. Following this, the cells were washed with modified KPBB-HEPES buffer (134 mM NaCl, 4.8 mM KCl, 1 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4, 5 mM NaHCO3, 10 mM HEPES, and 1 mg/mL BSA; pH 7.4) and then incubated in the same buffer supplemented with 20 mM glucose for 1.5 h. The supernatant was collected and centrifuged at 12,000 g for 10 min at 4°C and used for determination of insulin with an ELISA assay kit (Mercodia Rat Insulin Kit, Mercodia, Sweden). Cells were lysed in a lysis buffer [1% Triton-X, 20 mM HEPES, pH 7.4, 100 mM KCl, 2 mM EDTA, 1.0 mM PMSF, with protease inhibitors (Roche)]. Total protein of the cell extract was determined using a BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).
3
Insulin Quantification and Histological Analysis
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Insulin level was measured by using Mercodia rat insulin kit. On day 9 all animals were sacrificed and the liver and pancreas were excised. These organs were cut by sagital plane and stored in buffered 10% formalin solution. After routing processing, paraffin blocks were prepared for liver and pancreas separately. Sections with 5μm thickness were stained with hematoxylin and eosin (H & E) respectively and were studied under light microscope.
4
Metabolic Biomarkers Measurement Protocol
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The serum levels of the triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), and serum glucose (Glc) were measured using the Randox assay kit (Randox Laboratories, Ltd., UK). Low-density lipoprotein cholesterol (LDL-c) was then calculated according to the Friedewald equation: TC-(HDL-C+1/5 TGs). The serum insulin and C-peptide levels were compared by ELISA (enzyme-linked immunosorbent assay) method using a rat insulin kit (Mercodia, Uppsala, Sweden). Total hemoglobin total proteins and glycosylated hemoglobin was estimated using a procedure described previously [22 ].
5
Glucose and Insulin Homeostasis Assessment
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Glucose and insulin concentrations were analyzed using a glucose hexokinase kit (Bayer, Pittsburgh, PA, USA) and a rat insulin kit (Mercodia AB, Uppsala, Sweden), respectively, in accordance with the manufacturers’ instructions. The homeostatic model assessment (HOMA)-insulin resistance (IR) index was calculated using the glucose and insulin concentrations, according to the following equation: HOMA-IR = (fasting serum glucose (mmol/L) × fasting serum insulin (μU/mL))/22.5
6
Metabolic Profiling and Insulin Resistance
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Fasting blood sugar (FBS), triglyceride (TG), total cholesterol (TC), LDL, HDL, serum creatinine (Cr), and protein excretion in urine (PU) were measured by photometric methods. Moreover, the atherogenic index was computed with LDL/HDL ratio. The serum insulin level was determined by the enzyme-linked immunosorbent assay (ELISA) method using a rat insulin kit (Mercodia, Uppsala, Sweden) and HOMA-IR (homeostasis model assessment of insulin resistance) was calculated as explained previously (4 (link)).
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Fasting blood sugar (FBS), triglyceride (TG), total cholesterol (TC), LDL, HDL, serum creatinine (Cr), and protein excretion in urine (PU) were measured by photometric methods. Moreover, the atherogenic index was computed with LDL/HDL ratio. The serum insulin level was determined by the enzyme-linked immunosorbent assay (ELISA) method using a rat insulin kit (Mercodia, Uppsala, Sweden) and HOMA-IR (homeostasis model assessment of insulin resistance) was calculated as explained previously (4 (link)).
7
Metabolic Characterization of Goto-Kakizaki Rats
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Four weeks old Goto-Kakizaki rats (GK/MolTac, Aarhus colony) and Wistar Han rats were purchased from Taconic Farms, Inc., Hudson, NY and kept in the IKEM breeding facility in a temperaturecontrolled environment with a 12 h light/dark cycle. The animals were randomized into 4 subgroups each comprising 6-8 animals and assayed at the age of 6, 20, 40, and 52 weeks. The animals had free access to the standard laboratory chow diet, and to the tap water. All tests including weighing, blood sampling, and glucose tolerance tests were initiated between 8-9 a.m. on random fed animals. Blood glucose concentration was measured by Accu-chek glucometer, insulin concentrations were determined using Rat Insulin kit (Mercodia, Sweden). Adiposity was defined as the wet weight of epididymal fat pads normalized per 100 g body weight (Sedova et al. 2004) . Triacylglycerol content in the liver was determined as described previously (Cahova et al. 2004) . All experiments were performed in accordance with the Animal Protection Law of the Czech Republic 311/1997, which is in compliance with the Principles of Laboratory Animal Care (NIH Guide to the Care and Use of Laboratory Animals, 8 th edition, 2013) and were approved by the Ethical Committee of IKEM (permission no. 26/2012).
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