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Mouse pm20d1 flag

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The Mouse PM20D1-flag is a recombinant protein that contains the PM20D1 gene sequence from the mouse and a FLAG tag. PM20D1 is an enzyme involved in the metabolism of N-acyl amino acids. The inclusion of the FLAG tag allows for the detection and purification of the PM20D1 protein.

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Lab products found in correlation

N arachidonoyl glycine, by Cayman Chemical (2 mentions) Penn aav tmck pi egfp wpre bgh, by Addgene (2 mentions) Irdye 680rd anti mouse igg, by LI COR (2 mentions) Anti v5 antibody, by Thermo Fisher Scientific (2 mentions) Pcag cre, by Addgene (2 mentions) Anti β actin, by Abcam (2 mentions) Mouse anti flag m2 antibody, by Merck Group (2 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (2 mentions) Aav tbg pi cre rbg, by Addgene (2 mentions) Anti bhmt, by Abcam (2 mentions) Anti rabbit igg irdye 800rd, by LI COR (2 mentions) Anti biotin streptavidin alexafluor 680, by Thermo Fisher Scientific (2 mentions) V5 turboid nes pcdna3, by Addgene (2 mentions) Human fgf1 myc ddk, by OriGene (2 mentions) Human flag fgf2, by Sino Biological (2 mentions) Penn aav tmck pi egfp wpre bgh, by Thermo Fisher Scientific (2 mentions) Aav tbg pi cre rbg, by Thermo Fisher Scientific (2 mentions) Anti beta tubulin antibody, by Abcam (2 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Arachidonic acid, by Merck Group (1 mentions)

4 protocols using mouse pm20d1 flag

1

Synthesis and Characterization of N-Acyl Amino Acids

Cited 2 times
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N-arachidonoyl glycine, N-arachidonoyl serine, and N-arachidonoyl-taurine were purchased from Cayman. N-arachidonoyl phenylalanine was purchased from Abcam. Arachidonic acid, anandamide, and WWL70 were purchased from Sigma-Aldrich. PF-3845 was purchased from Selleckchem. MAFP and EDTA were purchased from Fisher. Talabostat was purchased from R and D. Non-commercially available N-acyl amino acids were synthesized as previously described (Lin et al., 2018 (link); Long et al., 2016 (link)). Plasmids were obtained from the following sources: mouse PM20D1-flag (Addgene 132682), mouse FAAH-flag (Origene MR209084), mouse ACY1-flag (Origene MR206415), mouse CNDP1-flag (Origene MR219018), mouse CNDP2-flag (Origene MR207616), and mouse PM20D2-flag (MR222068).
Kim J.T., Terrell S.M., Li V.L., Wei W., Fischer C.R, & Long J.Z. (2020). Cooperative enzymatic control of N-acyl amino acids by PM20D1 and FAAH. eLife, 9, e55211.
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2

Comprehensive Antibody and Plasmid Resource

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The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804), anti-beta Actin (Abcam ab8227), anti-beta Tubulin antibody (Abcam ab6046), anti-Albumin (Abcam ab19194), anti-BHMT (Abcam 96415), anti-mouse IgG IRDye 680RD (LI-COR 925-68070), anti-rabbit IgG IRDye 800RD (LI-COR 926-32211), anti-biotin Streptavidin AlexaFluor-680 (ThermoFisher S32358), anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556), AAV.TBG.PI.Cre.rBG (Addgene 107787-AAV8), pCAG-Cre (Addgene 13775), V5-TurboID-NES_pCDNA3 (Addgene 107169), human FGF1-myc-DDK (Origene RC207434), human flag-FGF2 (SinoBiological HG10014-NF). ER-TurboID and Mem-TurboID were synthesized (IDT) and inserted into pcDNA3, pENN.AAV.tMCK.PI.eGFP.WPRE.bGH, and AAV.TBG.PI.Cre.rBG expression plasmids with pENTR-d-topo cloning (ThermoFisher K240020) and restriction site cloning. mBHMT-flag was synthesized (IDT) and inserted into pcDNA-DEST40 expression plasmids with pENTR-d-topo cloning. The following adeno-associated viruses (and batch numbers in parentheses) were produced at the Penn Vector Core: AAV8-Tbg-Mem (V7155S), AAV8-Tbg-Cyto (V7119S), AAV8-Tbg-ER (V7069S), AAV9-tMCK-ER (V7074S), AAV8-CB7-FLEx (V7258S).
Wei W., Riley N.M., Yang A.C., Kim J.T., Terrell S.M., Li V.L., Garcia-Contreras M., Bertozzi C.R, & Long J.Z. (2020). Cell type-selective secretome profiling in vivo. Nature chemical biology, 17(3), 326-334.
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3

Comprehensive Antibody and Plasmid Resource

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The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804), anti-beta Actin (Abcam ab8227), anti-beta Tubulin antibody (Abcam ab6046), anti-Albumin (Abcam ab19194), anti-BHMT (Abcam 96415), anti-mouse IgG IRDye 680RD (LI-COR 925-68070), anti-rabbit IgG IRDye 800RD (LI-COR 926-32211), anti-biotin Streptavidin AlexaFluor-680 (ThermoFisher S32358), anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556), AAV.TBG.PI.Cre.rBG (Addgene 107787-AAV8), pCAG-Cre (Addgene 13775), V5-TurboID-NES_pCDNA3 (Addgene 107169), human FGF1-myc-DDK (Origene RC207434), human flag-FGF2 (SinoBiological HG10014-NF). ER-TurboID and Mem-TurboID were synthesized (IDT) and inserted into pcDNA3, pENN.AAV.tMCK.PI.eGFP.WPRE.bGH, and AAV.TBG.PI.Cre.rBG expression plasmids with pENTR-d-topo cloning (ThermoFisher K240020) and restriction site cloning. mBHMT-flag was synthesized (IDT) and inserted into pcDNA-DEST40 expression plasmids with pENTR-d-topo cloning. The following adeno-associated viruses (and batch numbers in parentheses) were produced at the Penn Vector Core: AAV8-Tbg-Mem (V7155S), AAV8-Tbg-Cyto (V7119S), AAV8-Tbg-ER (V7069S), AAV9-tMCK-ER (V7074S), AAV8-CB7-FLEx (V7258S).
Wei W., Riley N.M., Yang A.C., Kim J.T., Terrell S.M., Li V.L., Garcia-Contreras M., Bertozzi C.R, & Long J.Z. (2020). Cell type-selective secretome profiling in vivo. Nature chemical biology, 17(3), 326-334.
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4

N-arachidonoyl glycine photocrosslinking probe

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N-arachidonoyl glycine was purchased from Cayman. N-acyl amino acid photocrosslinking probe was synthesized as previously described (Long et al., 2016 ). Phenylalanine (BP391100) and oleic acid (AC270290050) were purchased from Fisher. BSA (protease free, fatty acid free, essentially globulin free) was purchased from Sigma (A7030). Pooled human plasma (IPLA-N) was purchased from Innovative Research. The following antibodies were used: anti-Flag M2 (Sigma F3165), anti-APOB (Abcam ab20737), anti-APOA1 (ab7614). The following plasmids were used: mouse AAV-PM20D1-flag (Addgene 132682), mouse PM20D1-flag (Addgene 84566). Adeno-associated viruses were produced at the Penn Vector Core. AAV-GFP virus was purchased from Penn Vector Core (AAV8.CB7.CI.eGFP.WPRE.rBG).
Kim J.T., Jedrychowski M.P., Wei W., Fernandez D., Fischer C.R., Banik S.M., Spiegelman B.M, & Long J.Z. (2020). A plasma protein network regulates PM20D1 and N-acyl amino acid bioactivity. Cell chemical biology, 27(9), 1130-1139.e4.
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