Tissue sections were deparaffinized, rehydrated, and heat-treated with citrate buffer (0.01 M, pH 6.0) for epitope retrieval. After exposure to 3% hydrogen peroxide for 10 minutes to block endogenous peroxidase activity, the tissue sections were incubated with anti-HOXA13 (1:200; Abcam) and anti-Ki67 antibodies (1:100; Abcam) at 4° overnight. Then, the anti-mouse or anti-rabbit GTVision™ Detection System (GeneTech, Shanghai, China) was used to detect the primary antibody after incubation for 30 minutes at room temperature. After washing, the slides were counterstained with hematoxylin and mounted with coverslips. The immunohistochemical score was based on the staining intensity and the percentage of positive cells.14 (link)
Gtvision detection system
Manufactured by Gene Tech
Sourced in China
The GTVision™ Detection System is a laboratory equipment designed for sensitive and accurate detection of target analytes. The system utilizes advanced optical and electronic technologies to provide reliable and reproducible results.
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Lab products found in correlation
2 protocols using gtvision detection system
1
Immunohistochemical Analysis of HOXA13 and Ki67
2
SLCO4A1-AS1 Expression and Signaling in CRC
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The fluorescent in situ detection of SLCO4A1-AS1 was performed on HCT116 cells and 4 µm fresh frozen tissue slides according to the manufacture’s protocol provided by Ribo™ Fluorescent In Situ Hybridization Kit (RiboBio). IHC staining was performed on 4-μm sections of the tissue microarrays constructed using paraffin-embedded CRC tissue samples with primary antibodies for Cdk2 (1:50, Proteintech) or Phospho-c-Myc (62) (p-62 c-Myc, 1:100, Abmart). Next, the expression levels of Cdk2 and p-62 c-Myc were quantified using the GTVision™ Detection System (Gene Tech, China). The total Cdk2 and p-62 c-Myc immunostaining scores were evaluated based on the percentage of positively stained cells and the staining intensity. The percent positivity was scored on a scale of 0–4 as follows: 0 (0%), 1 (1–25%); 2 (26–50%; 3(51–75%) and 4 (> 75%). The staining intensity was established from 0 to 3, with 0 for no staining, 1 for weakly stained, 2 for moderately stained, and 3 for strongly stained. Both the percent positivity of cells and the staining intensity were evaluated under double-blind conditions by two independent pathologists.
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