Pan plcγ2

Manufactured by Bioworld Technology

Pan-PLCγ2 is a laboratory reagent used for the detection and quantification of PLCγ2, a key enzyme involved in various cellular signaling pathways. It can be utilized in various biochemical and molecular biology techniques to study the expression and activity of PLCγ2 in biological samples.

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Lab products found in correlation

Anti tyr 1217, by Bioworld Technology (3 mentions) Anti tyr 416, by Cell Signaling Technology (2 mentions) Pan c src, by Proteintech (2 mentions) Anti tyr 525, by Bioworld Technology (2 mentions) Pan syk, by Bioworld Technology (2 mentions) Caspase 1, by Abcam (1 mentions) Il 1β, by Cell Signaling Technology (1 mentions) Pan akt, by Affinity Biosciences (1 mentions)

3 protocols using pan plcγ2

Platelet Function and Signaling Assays

Cited 1 time

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Platelet receptor expression, activation, aggregation and immunoblotting were studied as previously described.^{17 (link),18 (link)} Antibodies against c-Src (anti-Tyr-416, Cell Signaling Technology; pan-c Src, Proteintech), Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology) and PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology), IL-1β (Cell Signaling Technology) and Caspase-1 (BioVision) were used.
Detailed methods of the electron microscopy of platelet spreading, and clot retraction are provided in the Online Supplement.

Qiao J., Wu X., Luo Q., Wei G., Xu M., Wu Y., Liu Y., Li X., Zi J., Ju W., Fu L., Chen C., Wu Q., Zhu S., Qi K., Li D., Li Z., Andrews R.K., Zeng L., Gardiner E.E, & Xu K. (2018). NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis. Haematologica, 103(9), 1568-1576.

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Quantification of Phosphorylated Signaling Proteins

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Levels of total and phosphorylated c-Src (anti-Tyr-416, Cell Signaling Technology; pan-c Src, Proteintech), Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology), PLCγ2 (anti-Tyr-1217 and pan-PLCγ2, Bioworld Technology), AKT (anti-Thr308 and anti-Ser473, Cell Signaling Technology; pan-AKT, Affinity Biosciences), or GSK-3β (anti-Ser9, Cell Signaling Technology; pan-GSK-3β, Affinity Biosciences) were assessed by SDS-PAGE/western blot. The density of protein band was quantified using Image J software and the phosphorylation level was shown as a ratio to the total level.

Wei G., Xu X., Tong H., Wang X., Chen Y., Ding Y., Zhang S., Ju W., Fu C., Li Z., Zeng L., Xu K, & Qiao J. (2020). Salidroside inhibits platelet function and thrombus formation through AKT/GSK3β signaling pathway. Aging (Albany NY), 12(9), 8151-8166.

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Platelet Receptor Expression and Signaling

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Washed human platelets were treated with different concentrations of PD followed by measuring the expression of α_IIbβ₃, GPIbα and GPVI by western blot using rabbit polyclonal CD41/Integrin Alpha 2b Antibody (Proteintech, Rosemont, IL, USA), mouse monoclonal CD42b antibody (SZ2) (Santa Cruz, Dallas, Texas, USA) or GP6 Monoclonal Antibody (HY101) (Ebioscience, San Diego, CA, USA) respectively. For some experiments, washed platelets were treated with CRP (5 μg/ml) or thrombin (1 U/ml) in the presence different concentrations of PD or vehicle for 15 min. Levels of total and phosphorylated Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology) and PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology) were assessed by SDS-PAGE/western blot.

Luo Q., Wei G., Wu X., Tang K., Xu M., Wu Y., Liu Y., Li X., Sun Z., Ju W., Qi K., Chen C., Yan Z., Cheng H., Zhu F., Li Z., Zeng L., Xu K, & Qiao J. (2018). Platycodin D inhibits platelet function and thrombus formation through inducing internalization of platelet glycoprotein receptors. Journal of Translational Medicine, 16, 311.

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