Cellular migration was tracked in real-time using xCELLigence RTCA Analyzer (ACEA Biosciences) and Cell Invasion and Migration (CIM) plates (ACEA Biosciences). The lower chamber of the CIM plate was filled with growth medium supplemented with 5% FBS serving as chemoattractant. Serum-free media was used as negative control for the migration. Serum-starved (20 h) C6 cells were seeded out to the upper chamber of CIM plate at a density of 3 × 104 cells per well and immediately treated with the compounds of interest. The cells were allowed to migrate via microporous polyethylene terephthalate (PET) membrane for up to 24 h. During that time, the impedance of the gold microelectrode array attached to the bottom side of the membrane was monitored independently for each well and converted by RTCA Software v1.2 (ACEA Biosciences) to cell index (CI), a dimensionless parameter, which was directly proportional to the total area of microelectrodes populated by the cells. The CI values were plotted over time and the slope of the obtained curves was calculated by the software, providing the information on the migration rate.
Xcelligence rtca analyzer
Manufactured by Agilent Technologies
The XCELLigence RTCA Analyzer is a real-time cell analysis system designed to monitor cell behavior in real-time. It measures the impedance of adherent cells, which correlates with cell number, morphology, and adhesion. The system provides quantitative data on various cell parameters without the need for labeling or specialized sample preparation.
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Lab products found in correlation
2 protocols using xcelligence rtca analyzer
1
Real-Time Tracking of Cellular Migration
2
Dermal Papilla Cell Proliferation Assay
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Dermal papilla cells were digested with 0.25% trypsin and counted. A 50 µL volume of complete medium was added to a 16-well E-plate, and background impedance was measured using RTCA for 15–30 minutes. Following a 10–15-minute background equilibration, dermal papilla cells were seeded into E-plate proliferation plates at different densities (8000 cells per well) in a 50 µL volume. The plates were left at room temperature for 30 minutes to facilitate passive adherence of the cells to the gold electrode-coated bottom of the electronic board. Afterward, the electronic board was placed in the RTCA unit (xCELLigence RTCA Analyzer; ACEA Biosciences) within a 5% CO2 humidified incubator. Cell impedance was monitored every 5 minutes for a duration of over 100 hours.
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