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Trcn0000330971

Manufactured by Merck Group
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TRCN0000330971 is a laboratory equipment product. It is a tool designed for use in scientific research and testing applications. The core function of this product is to provide a specific capability required for laboratory work, but without further details on its intended use or interpretation of its features.

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Lab products found in correlation

Shc001, by Merck Group (2 mentions) Cm3050, by Leica camera (2 mentions) Pcagig venus, by Thermo Fisher Scientific (2 mentions) F13082, by Thermo Fisher Scientific (2 mentions)

2 protocols using trcn0000330971

1

Manipulating Neuronal Progenitors in Cerebral Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organoids (specifically, the “ventricles” or progenitor zones) were electroporated with either a MeCP2 shRNA construct (validated sequence from Sigma; TRCN0000330971) or control (SHC001, Sigma), each co-injected with a Venus construct (pCAGIG-Venus; gift from Omar Durak and Prof. Li-Huei Tsai) at 8 weeks post-EB formation. Indicated plasmids were mixed at the following concentrations: shRNA (shMeCP2) / control (shControl) plasmids, 1 μg/ μl; pCAG-Venus plasmid, 0.5 μg/μl. Immediately after DNA injection into the organoid, four 50-ms electrical pulses (40V) were applied at 1-s intervals using a 5-mm electrode and an electroporator (EM830, BTX). After 7 days, organoids were fixed (4% PFA) and cryoprotected in 20% and 30% sucrose solutions, respectively, overnight. Fixed organoids were sliced on a cryostat (Leica, CM 3050 S) into 20 μM sections. For tracing migration of newly born neurons, cerebral organoids from Rett-WT2 and RTT-Mut2 cells were co-injected with pCAGIG-Venus and fluorescence beads (Molecular Probes; F13082) that mark the location of the injected ventricles.
Mellios N., Feldman D.A., Sheridan S.D., Ip J.P., Kwok S., Amoah S.K., Rosen B., Rodriguez B.A., Crawford B., Swaminathan R., Chou S., Li Y., Ziats M., Ernst C., Jaenisch R., Haggarty S.J, & Sur M. (2017). MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling. Molecular psychiatry, 23(4), 1051-1065.
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2

Manipulating Neuronal Progenitors in Cerebral Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organoids (specifically, the “ventricles” or progenitor zones) were electroporated with either a MeCP2 shRNA construct (validated sequence from Sigma; TRCN0000330971) or control (SHC001, Sigma), each co-injected with a Venus construct (pCAGIG-Venus; gift from Omar Durak and Prof. Li-Huei Tsai) at 8 weeks post-EB formation. Indicated plasmids were mixed at the following concentrations: shRNA (shMeCP2) / control (shControl) plasmids, 1 μg/ μl; pCAG-Venus plasmid, 0.5 μg/μl. Immediately after DNA injection into the organoid, four 50-ms electrical pulses (40V) were applied at 1-s intervals using a 5-mm electrode and an electroporator (EM830, BTX). After 7 days, organoids were fixed (4% PFA) and cryoprotected in 20% and 30% sucrose solutions, respectively, overnight. Fixed organoids were sliced on a cryostat (Leica, CM 3050 S) into 20 μM sections. For tracing migration of newly born neurons, cerebral organoids from Rett-WT2 and RTT-Mut2 cells were co-injected with pCAGIG-Venus and fluorescence beads (Molecular Probes; F13082) that mark the location of the injected ventricles.
Mellios N., Feldman D.A., Sheridan S.D., Ip J.P., Kwok S., Amoah S.K., Rosen B., Rodriguez B.A., Crawford B., Swaminathan R., Chou S., Li Y., Ziats M., Ernst C., Jaenisch R., Haggarty S.J, & Sur M. (2017). MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling. Molecular psychiatry, 23(4), 1051-1065.
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