Anti foxm1 antibody

Manufactured by Abcam

Sourced in United States

Anti-FOXM1 antibody is a primary antibody that specifically binds to and detects the FOXM1 protein. FOXM1 is a transcription factor that plays a key role in cell proliferation, cell cycle regulation, and tumor development. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of FOXM1 in biological samples.

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FOXM1 (20 citations) Anti-FOXM1 (12 citations)

5 protocols using anti foxm1 antibody

Curcumin Regulates Gastric Cancer Cell Signaling

Cited 1 time

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The human gastric cancer cell line, SGC-7901 was obtained from the Laboratory of Pathology, School of Basic Medical, Lanzhou University (Lanzhou, China),31 (link) and the cells were authenticated by STR. Cells were cultured in RPIM-1640 (HyClone, UT, USA) supplemented with 10% fetal bovine serum (FBS; Kibbutz Beit Haemek, Israel) and 1% penicillin/streptomycin (Sigma-Aldrich, MO, USA) in a humidified atmosphere of 5% CO₂ at 37°C. Curcumin and a CCK-8 kit were purchased from Beijing Solarbio Science & Technology (Beijing, China).
Primary antibodies included: Anti-Shh (Abcam, Cambridge, UK), anti-Gli1 antibody (Abcam), anti-Foxm1 antibody (Abcam), anti-β-catenin antibody (Cell Signaling Technology, MA, USA), anti-E-Cadherin antibody (Cell Signaling Technology), anti-vimentin antibody (Cell Signaling Technology), anti-F-actin antibody (Abcam) and anti-β-actin antibody (Thermo Fisher Scientific, MA, USA). Secondary antibodies included: HRP-labeled goat anti-rabbit IgG (Abcam) and HRP-labeled goat anti-mouse IgG (Abcam). All the primary antibodies were diluted to 1:1000. The secondary antibodies were diluted to 1:5000.

Zhang X., Zhang C., Ren Z., Zhang F., Xu J., Zhang X, & Zheng H. (2020). Curcumin Affects Gastric Cancer Cell Migration, Invasion and Cytoskeletal Remodeling Through Gli1-β-Catenin. Cancer Management and Research, 12, 3795-3806.

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Immunohistochemical Analysis of Cancer Protein Expression

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Immunohistochemistry (IHC) was performed to assess protein expression in cancer tissues according to previous report [26 (link)]. In brief, the formalin-fixed, paraffin-embedded tissues were cut into 4-μm sections, which were de-waxed, dehydrated, and retrieved by antigen-retrieval liquid. Then, the sections were blocked with 5% goat serum for 1 hour at room temperature, followed by incubation with indicated primary antibodies at 4°C overnight and second antibodies at 37°C for 30 min. The primary antibodies included anti-SPAG5 antibody (1:100 dilution, cat no. 14,726-1-AP, Proteintech, USA), anti-FOXM1 antibody (1:250 dilution, cat no. ab207298, Abcam, Cambridge, MA, USA), anti-ADAM17 antibody (1:100 dilution; cat no. ab39163, Abcam), ant-NOTCH1 antibody (1:150 dilution, cat no. ab52627, Abcam), anti-E-cadherin antibody (1:200 dilution; cat no. ab231303, Abcam) and anti-N-cadherin antibody (1:150 dilution; cat no. ab76011, Abcam). After that, the sections were reacted with diaminobezidin (DAB) for several seconds at room temperature and hematoxylin (Solarbio, Beijing, China) for 1 min. The staining was observed by using a microscope.

Dang L., Shi C., Zhang Q., Liao P, & Wang Y. (2022). Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling. Bioengineered, 13(3), 4744-4756.

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Protein Analysis in HUVECs

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Protein was extracted from HUVECs and subjected to immunoblotting analysis of protein levels according to previous studies [29 (link), 30 (link)]. The following antibodies were used: a mouse monoclonal anti-β-actin antibody (1: 500, Cat#: A5441, Sigma-Aldrich, St Louis, MO, USA) and a rabbit polyclonal anti-FOXM1 antibody (1:500, Cat#: ab175798, Abcam, Cambridge, MA, USA).

Sun J.Y., Zhao Z.W., Li W.M., Yang G., Jing P.Y., Li P., Dang H.Z., Chen Z., Zhou Y.A, & Li X.F. (2017). Knockdown of MALAT1 expression inhibits HUVEC proliferation by upregulation of miR-320a and downregulation of FOXM1 expression. Oncotarget, 8(37), 61499-61509.

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Antibodies and Knockdown Analysis

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Antibodies against poly-(adenosine diphosphate-ribose) polymerase, caspase-3, and tubulin were purchased from Cell Signaling Technology (Danvers, MA). Cytochrome c and Prx3 antibodies were purchased from BD Pharmingen (San Jose, CA), and Abclone (Seoul, Korea), respectively. The anti-FoxM1 antibody was purchased from Abcam (Cambridge, MA, USA). Antibodies against CD133, including those conjugated to magnetic beads, and allophycocyanin (APC), were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
For Prx3 and FoxM1 knockdown, siRNAs (Prx3: 5′-AAGCCAAGTCCAGCTGCTTCC-3′, FoxM1: 5′-CTCTTCTCCCTCAGATATA-3′) were constructed based on the Prx3 and FoxM1 sequences, and exhibited > 80% knockdown efficiencies. For Prx3 overexpression, Ishikawa cells were transfected with 6 μg pCGN-HA or pCGN-HA-Prx3 for 48 hours using Lipofectamine LTX (Invitrogen, Carlsbad, CA) following the manufacturer's protocols, and then doxorubicin was treated with the indicated dosage for 24 hours. The transfected cells were harvested and subjected to western blotting with anti-PARP, anti-HA, and anti-tubulin antibodies.

Song I.S., Jeong Y.J., Seo Y.J., Byun J.M., Kim Y.N., Jeong D.H., Han J., Kim K.T, & Jang S.W. (2017). Peroxiredoxin 3 maintains the survival of endometrial cancer stem cells by regulating oxidative stress. Oncotarget, 8(54), 92788-92800.

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FOXM1 Subcellular Localization in Huh7 Cells

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Immunofluorescence analysis was performed using Huh7 cells, anti-FOXM1 antibody (Abcam), Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (Thermo Fisher Scientific), and DAPI. Super-resolution images were obtained using the Dragonfly Confocal Imaging Platform (Andor, Belfast, UK).

Li R., Okada H., Yamashita T., Nio K., Chen H., Li Y., Shimakami T., Takatori H., Arai K., Sakai Y., Yamashita T., Mizukoshi E., Honda M, & Kaneko S. (2022). FOXM1 Is a Novel Molecular Target of AFP-Positive Hepatocellular Carcinoma Abrogated by Proteasome Inhibition. International Journal of Molecular Sciences, 23(15), 8305.

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