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Anti phospho mapkapk 2

Manufactured by Cell Signaling Technology
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Anti-phospho-MAPKAPK-2 is a laboratory product that detects the phosphorylated form of the MAPKAPK-2 protein. MAPKAPK-2 is a serine/threonine-protein kinase that plays a role in the p38 MAPK signaling pathway. This product can be used to study the activation and regulation of MAPKAPK-2 in various cellular and biological systems.

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Lab products found in correlation

Anti cleaved caspase 9, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 7, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 8, by Cell Signaling Technology (2 mentions) Anti phospho mek1 2, by Cell Signaling Technology (2 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (2 mentions) Anti phospho c raf, by Cell Signaling Technology (2 mentions) Anti phospho mkk3 6, by Cell Signaling Technology (2 mentions) Anti phospho erk1 2, by Cell Signaling Technology (2 mentions) Anti p38 mapk, by Cell Signaling Technology (2 mentions) Anti erk1 2, by Cell Signaling Technology (2 mentions) Anti parp, by Cell Signaling Technology (2 mentions) Anti phospho c jun, by Cell Signaling Technology (2 mentions) Anti c jun, by Cell Signaling Technology (2 mentions) Anti myc, by Roche (1 mentions) Anti hnrnpd, by Merck Group (1 mentions) Anti igf2bp1, by Santa Cruz Biotechnology (1 mentions) Anti cdk9, by Santa Cruz Biotechnology (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Anti cnot1 14276 1 ap, by Proteintech (1 mentions) Anti phospho rsk, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti phospho mapkapk 2

1

Western Blot Antibody Analysis

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All chemicals were from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. For western blot analysis, the following primary and secondary antibodies were used: anti-phospho-MKK3/6 (#9236), anti-p38 MAPK (#8690), anti-phospho-p38 MAPK (#4511), anti-phospho-MAPKAPK-2 (#3007), anti-phospho-c-Raf (#9427), anti-phospho-MEK1/2 (#9154), anti-ERK1/2 (#5013), anti-phospho-ERK1/2 (#4370), anti-PARP (#9542), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505) from Cell Signaling Technology (Danvers, MA, USA) and anti-actin (clone AC-40).
Šrámek J., Němcová-Fürstová V., Balušíková K., Daniel P., Jelínek M., James R.F, & Kovář J. (2016). p38 MAPK Is Activated but Does Not Play a Key Role during Apoptosis Induction by Saturated Fatty Acid in Human Pancreatic β-Cells. International Journal of Molecular Sciences, 17(2), 159.
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2

Comprehensive Antibody Analysis Protocol

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The following antibodies were used for IP and/or western blot analysis: anti-β-actin (#4970; Cell Signalling), anti-CDK9 (sc-13130; Santa Cruz), anti-CNOT1 (14276-1-AP; Protein Tech), anti-CNOT7 (H00029883; Abnova), anti-FLAG (M2; Sigma), anti-hnRNPD (Q14103; Millipore), anti-HA (1867423; Roche), anti-IGF2BP1 (sc-21026; Santa Cruz), anti-KHSRP (ab83291; Abcam), anti-LARP7 (LaRP7-101AP; FabGennix Inc.), anti-LDLR (AF2148; BD Biosciences), anti-Myc (9E10; Roche), anti-phospho-ERK (#9101s; Cell Signalling), anti-phospho-MAPKAPK2 (#3007s; Cell Signalling), anti-phospho-RSK (sc-17033; Santa Cruz), anti-ZFP36L1 (#2119; Cell Signalling), anti-phospho-S6 (#2211; Cell Signalling).
Adachi S., Homoto M., Tanaka R., Hioki Y., Murakami H., Suga H., Matsumoto M., Nakayama K.I., Hatta T., Iemura S.I, & Natsume T. (2014). ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK–RSK pathway. Nucleic Acids Research, 42(15), 10037-10049.
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3

Western Blot Analysis of Stress Pathways

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Chemicals were from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. The following primary and secondary antibodies for Western blot analysis were used: anti-phospho-MKK3/6 (#9236), anti-p38 MAPK (#8690), anti-phospho-p38 MAPK (#4511), anti-phospho-MAPKAPK-2 (#3007), anti-phospho-c-Raf (#9427), anti-phospho-MEK1/2 (#9154), anti-ERK1/2 (#5013), anti-phospho-ERK1/2 (#4370), anti-BiP (#3177), anti-CHOP (#2895), anti-eIF2α (#9722), anti-phospho-eIF2α (#9261), anti-IRE1α (#3294), anti-SAPK/JNK (#9258), anti-phospho-SAPK/JNK (#4668), anti-c-Jun (#9165) and anti-phospho-c-Jun (#9261), anti-PARP (#9542), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505) from Cell Signaling Technology (Danvers, MA, USA), anti-phospho-IRE1α (ab 48187) and anti-GAPDH (ab9485) from Abcam (Cambridge, UK), and anti-actin (clone AC-40).
Šrámek J., Němcová-Fürstová V., Pavlíková N, & Kovář J. (2017). Effect of Saturated Stearic Acid on MAP Kinase and ER Stress Signaling Pathways during Apoptosis Induction in Human Pancreatic β-Cells Is Inhibited by Unsaturated Oleic Acid. International Journal of Molecular Sciences, 18(11), 2313.
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4

Antibody Validation for Signaling Proteins

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Anti-IL-20R1 antibody was purchased from abcam (Cat #ab203196) and anti-IL-20R2 was purchased from MyBioSource (Cat #MBS9605059). Anti-MAPKAPK-2 and anti-phospho-MAPKAPK-2 were purchased from Cell Signaling (Cat #3042P and #3007P, respectively). Anti-GAPDH was purchased from US Biological (Cat #G8140). Anti-c-Jun and anti-phospho-c-Jun were purchased from Cell Signaling (Cat #9165P and #2361P, respectively). Anti-phospho-H2AX (Ser139) was purchased from Millipore (Cat #05-636).
Small S.H., Tang E.J., Ragland R.L., Ruzankina Y., Schoppy D.W., Mandal R.S., Glineburg M.R., Ustelenca Z., Powell D.J., Simpkins F., Johnson F.B, & Brown E.J. (2021). Induction of IL19 expression through JNK and cGAS-STING modulates DNA damage–induced cytokine production. Science signaling, 14(714), eaba2611.
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