Hplc grade methanol

Manufactured by Carl Roth

Sourced in Germany

HPLC-grade methanol is a high-purity solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile, and flammable liquid with a characteristic odor.

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Lab products found in correlation

Formic acid, by Carl Roth (4 mentions) Calf intestine alkaline phosphatase, by Merck Group (3 mentions) 2 propanol, by Carl Roth (3 mentions) Acetic acid, by Carl Roth (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Bovine spleen phosphodiesterase, by Merck Group (2 mentions) Proteinase k, by Carl Roth (2 mentions) Hplc grade acetonitrile, by Carl Roth (1 mentions) Flurbiprofen flb, by Merck Group (1 mentions) Chloroform, by Carl Roth (1 mentions) L glutamine, by Carl Roth (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Resveratrol, by Merck Group (1 mentions) Fetal bovine serum (fbs), by PAN Biotech (1 mentions) 1 butanol, by Carl Roth (1 mentions) Herring sperm dna, by Merck Group (1 mentions) Ku55933, by Selleck Chemicals (1 mentions) Ucn 01, by Merck Group (1 mentions) Micrococcal nuclease, by Merck Group (1 mentions) Ve 821, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Methanol (143 citations) HPLC grade (6 citations) HPLC-grade methanol (MeOH) (2 citations)

15 protocols using hplc grade methanol

Flurbiprofen Metabolism Assay

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Dobendan Direkt lozenges (Boots Healthcare, Hamburg, Germany) were provided by Klosterfrau Healthcare Group, Köln, Germany. Water HPLC-grade, acetonitrile HPLC-grade, and methanol HPLC-grade were from Roth, Karlsruhe, Germany. Human CYP2C9*1 Supersomes were from BD Biosciences, Woburn, USA. Flurbiprofen (FLB) was purchased from Sigma-Aldrich, Steinheim, Germany, flurbiprofen-d₃ (FLB-d₃) was from TRC, Toronto, Canada. 4-Hydroxyflurbiprofen was a gift from the Dept. of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, USA. Primers were purchased from Invitrogen GmbH, Darmstadt, Germany. LongAmp Taq polymerase and restriction endonucleases AvaII, NsiI, and KpnI together with the respective ready-to-use buffers were purchased from New England Biolabs, Frankfurt a. Main, Germany.

Vogl S., Lutz R.W., Schönfelder G, & Lutz W.K. (2015). CYP2C9 Genotype vs. Metabolic Phenotype for Individual Drug Dosing—A Correlation Analysis Using Flurbiprofen as Probe Drug. PLoS ONE, 10(3), e0120403.

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Resveratrol Treatment in Cell Culture

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Dulbecco’s modified Eagle medium (DMEM) (#41965) and penicillin-streptomycin (#15140122) were purchased from Thermo Fisher Scientific, Darmstadt, Germany. l-glutamine (#56-85-9), methanol HPLC-grade (#7342.1), and chloroform (#3313.2) were ordered from Carl Roth, Karlsruhe, Germany. Fetal bovine serum (FBS) (#P30-3306) was obtained from PAN-Biotech GmbH, Aidenbach, Germany. D₂O (#STBJ4154), and resveratrol (#R5010) was ordered from Sigma-Aldrich/Merck, Taufkirchen, Germany. resveratrol was dissolved in polyethylene glycol 400 (PEG400) obtained from Caesar & Loretz GmbH, Hilden, Germany.

Schlotterose L., Pravdivtseva M.S., Ellermann F., Jansen O., Hövener J.B., Sönnichsen F.D., Cossais F., Lucius R, & Hattermann K. (2023). Resveratrol Mitigates Metabolism in Human Microglia Cells. Antioxidants, 12(6), 1248.

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Enzymatic Nucleic Acid Manipulation Protocol

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Calf
intestine alkaline phosphatase, micrococcal
nuclease (from Staphylococcus aureus), bovine spleen
phosphodiesterase, and ribonuclease A from bovine pancreas (RNase)
were purchased from Sigma-Aldrich (Steinheim, Germany). Proteinase
K, HPLC-grade methanol, 2-propanol, 1-butanol, formic acid, and acetic
acid were from Carl Roth GmbH (Karlsruhe, Germany). Herring sperm
DNA and all other reagents and solvents (analytical grade) were from
Sigma-Aldrich.

Monien B.H., Schumacher F., Herrmann K., Glatt H., Turesky R.J, & Chesné C. (2014). Simultaneous Detection of Multiple DNA Adducts in Human Lung Samples by Isotope-Dilution UPLC-MS/MS. Analytical Chemistry, 87(1), 641-648.

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DNA Damage Response Inhibitor Assay

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Calf intestine alkaline phosphatase, micrococcal nuclease, calf spleen phosphodiesterase and ribonuclease A (RNase A) were purchased from Sigma (Steinheim, Germany). Proteinase K, HPLC-grade methanol, formic acid and acetic acid were from Carl Roth GmbH (Karlsruhe, Germany). The synthesis of the isotope-labeled reference standard [¹⁵N₅,¹³C₁₀]C8-PhIP-dG was previously described (9 (link)). The CHK1 inhibitor UCN-01 was obtained from Sigma. The ATR inhibitor VE821, the ATM inhibitor KU-55933 and the DNA-PK_cs inhibitor NU7026 were from Selleck Chemicals (USA).

Mimmler M., Peter S., Kraus A., Stroh S., Nikolova T., Seiwert N., Hasselwander S., Neitzel C., Haub J., Monien B.H., Nicken P., Steinberg P., Shay J.W., Kaina B, & Fahrer J. (2016). DNA damage response curtails detrimental replication stress and chromosomal instability induced by the dietary carcinogen PhIP. Nucleic Acids Research, 44(21), 10259-10276.

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Sulfation of HMF and FFA for Analysis

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FFA was purchased from Merck Schuchardt (Hohenbrunn, Germany). HMF, adenosine, ammonium bicarbonate and all other reagents (analytical grade) were from Sigma-Aldrich (Taufkirchen, Germany). HPLC-grade methanol, 2-propanol and formic acid were from Carl Roth GmbH (Karlsruhe, Germany), and HPLC-grade water was prepared using a Milli-Q Integral Water Purification System (Millipore Merck, Darmstadt, Germany). Stable isotope-labeled [¹⁵N₅]adenosine was from Silantes GmbH (Munich, Germany). Sulfation reactions of HMF and FFA yielding SMF (Monien et al. 2009 (link)) and 2-sulfoxymethylfuran (Monien et al. 2011 (link)), respectively, were conducted as described. The cofactor 3′-phosphoadenosine-5′-phosphosulfate (PAPS) was prepared using human PAPS synthetase 2 expressed in Escherichia coli and was purified using anion-exchange chromatography (purity > 99.0 %) (Muckel et al. 2001 ).

Sachse B., Meinl W., Sommer Y., Glatt H., Seidel A, & Monien B.H. (2014). Bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol by sulfotransferases from human, mouse and rat: a comparative study. Archives of Toxicology, 90(1), 137-148.

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HPLC-grade Reagents Protocol

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HPLC-Grade methanol and water were purchased from Carl Roth (Karlsruhe, Germany). 2,5-Dihydroxybenzoic acid (DHB), 2-Sulfanylbenzoic acid (thiosalicylic acid, TSA), sucrose and acrylamide analytical standard were purchased from Sigma Aldrich (Dreieich, Germany). Adhesive glass slides (Menzel Gläser, SuperFrost) were purchased from VWR (Darmstadt, Germany).

Wittek O, & Römpp A. (2023). Autofocusing MALDI MS imaging of processed food exemplified by the contaminant acrylamide in German gingerbread. Scientific Reports, 13, 5400.

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Feather Corticosterone Extraction Method

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To examine the required quantity of methanol for a complete feather corticosterone extraction (Romero and Fairhurst, 2016 ), a pool of 38 interscapular feathers plucked from 1 laying hen was pulverized using a ball mill (MM-400; Retsch, Germany; also used by Ganz et al., 2018 ; see Table 1). Twenty-five replicates were created; of which, 5 were treated with 0.5 mL, 1.0 mL, 5.0 mL, 10.0 mL, or 15.0 mL HPLC-grade methanol (Carl Roth GmbH + Co. KG) each (based on the study by Newman and Freeman, 2018 ). Processing and analyzing of the replicates followed the procedure as described previously.

Häffelin K.E., Lindenwald R., Kaufmann F., Döhring S., Spindler B., Preisinger R., Rautenschlein S., Kemper N, & Andersson R. (2020). Corticosterone in feathers of laying hens: an assay validation for evidence-based assessment of animal welfare. Poultry Science, 99(10), 4685-4694.

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Feather Corticosterone Extraction Protocol

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Investigations regarding the influence of the crushing method (Newman and Freeman, 2018 ) were performed using a pool of 10 tail feathers taken from 1 animal (see Table 1). All feathers were minced using scissors (following Bortolotti et al., 2008 ) and then vortexed. Half of the amount of the minced and vortexed feathers were further processed and pulverized using a ball mill (MM-400; Retsch, Germany; also used by Ganz et al., 2018 ). Therefore, the replicate was placed into a metal container, frozen in liquid nitrogen for 3 min to embrittle, and then pulverized for 1 min at 30 Hz. The minced and pulverized samples were divided into 5 replicates each and enriched with 5.0 mL HPLC-grade methanol (Carl Roth GmbH + Co. KG) to extract feather corticosterone as mentioned previously.

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Quantification of Doxorubicin and Daunorubicin

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Doxorubicin hydrochloride was purchased from Ebewe (Unterach, Austria), daunorubicin hydrochloride, analytical grade chloroform, HPLC grade acetonitrile, orthophosphoric acid and hydrochloric acid were purchased from Sigma Chemical Company (St. Louis, Mo, USA). HPLC grade methanol was purchased from Carl ROTH (Karlsruhe, Germany).

Zaleskis G., Garberytė S., Pavliukevičienė B., Valinčius G., Characiejus D., Mauricas M., Kraśko J.A., Žilionytė K., Žvirblė M, & Pašukonienė V. (2020). Doxorubicin uptake in ascitic lymphoma model: resistance or curability is governed by tumor cell density and prolonged drug retention. Journal of Cancer, 11(22), 6497-6506.

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Preparation of Isotope-Labeled DNA Adduct

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FFA (CAS: 98-00-0) of 98% purity (Sigma-Aldrich, Steinheim, Germany) was diluted in saline shortly before use. Proteinase K and ribonuclease (RNase) A were purchased from Qiagen (Hilden, Germany). Calf intestine alkaline phosphatase, micrococcal nuclease (from Staphylococcus aureus) and bovine spleen phosphodiesterase were purchased from Sigma-Aldrich. HPLC-grade methanol, 2-propanol, formic acid and acetic acid were from Carl Roth GmbH (Karlsruhe, Germany). The syntheses of the isotope-labelled reference standard [¹⁵N₅,¹³C₁₀]N²-MF-dG has been described previously (22 (link)).

Høie A.H., Monien B.H., Sakhi A.K., Glatt H., Hjertholm H, & Husøy T. (2015). Formation of DNA adducts in wild-type and transgenic mice expressing human sulfotransferases 1A1 and 1A2 after oral exposure to furfuryl alcohol. Mutagenesis, 30(5), 643-649.

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