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Anti rhesus monkey cd3 fluorescein isothiocyanate clone sp34

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Anti-rhesus monkey CD3-fluorescein isothiocyanate (clone SP34) is a fluorescently labeled monoclonal antibody that binds to the CD3 antigen expressed on the surface of rhesus monkey T cells. It is used for the identification and enumeration of T cells in flow cytometry applications.

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Lab products found in correlation

Au480 chemistry analyzer, by Beckman Coulter (2 mentions) Xt 2000i, by Sysmex (2 mentions) Anti human cd4 phycoerythrin sk3, by BD (2 mentions) Anti human cd8 peridinin chlorophyll protein sk1, by BD (2 mentions)

Spelling variants of this product

Anti-CD3 (541 citations) Anti-CD3-fluorescein isothiocyanate (5 citations) CD3 − fluorescein isothiocyanate (2 citations)

2 protocols using anti rhesus monkey cd3 fluorescein isothiocyanate clone sp34

1

Vaccine Safety Assessment Protocol

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Body weight, blood chemistry and cell counts were measured monthly to assess vaccine safety. EDTA-anticoagulated blood was analyzed using a Sysmex XT2000i for blood cell counts. CD4+ and CD8+ cell counts were measured by flow cytometry with the following fluorochrome-labeled monoclonal antibodies: anti-rhesus monkey CD3-fluorescein isothiocyanate (clone SP34; Pharmingen), anti-human CD4-phycoerythrin (SK3; Becton Dickinson), and anti-human CD8-peridinin chlorophyll protein (SK1; BectonDickinson) as described before.23 (link) Blood chemistry was performed using the Beckman AU480 chemistry analyzer. Data are presented as mean ± SD.
Dumonteil E., Herrera C, & Marx P.A. (2022). Safety and preservation of cardiac function following therapeutic vaccination against Trypanosoma cruzi in rhesus macaques. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 56(2), 400-407.
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2

Vaccine Safety Assessment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Body weight, blood chemistry and cell counts were measured monthly to assess vaccine safety. EDTA-anticoagulated blood was analyzed using a Sysmex XT2000i for blood cell counts. CD4+ and CD8+ cell counts were measured by flow cytometry with the following fluorochrome-labeled monoclonal antibodies: anti-rhesus monkey CD3-fluorescein isothiocyanate (clone SP34; Pharmingen), anti-human CD4-phycoerythrin (SK3; Becton Dickinson), and anti-human CD8-peridinin chlorophyll protein (SK1; BectonDickinson) as described before.23 (link) Blood chemistry was performed using the Beckman AU480 chemistry analyzer. Data are presented as mean ± SD.
Purugganan M.M., Shah S., Kearney J.F, & Roth D.B. (2001). Ku80 is required for addition of N nucleotides to V(D)J recombination junctions by terminal deoxynucleotidyl transferase. Nucleic Acids Research, 29(7), 1638-1646.
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