Anti plc γ1 py783

Manufactured by Cell Signaling Technology

Anti-PLC-γ1 pY783 is a primary antibody that specifically recognizes phosphorylated tyrosine 783 in the PLC-γ1 protein. PLC-γ1 is an enzyme involved in signal transduction pathways.

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Lab products found in correlation

Anti src py416, by Cell Signaling Technology (2 mentions) Dylight 800, by Thermo Fisher Scientific (2 mentions) Dylight 680, by Thermo Fisher Scientific (2 mentions) Anti erk1 2 ptpy185 187, by Thermo Fisher Scientific (2 mentions) Anti gapdh, by Meridian Bioscience (2 mentions) Annexin 5, by BD (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti stat4, by Cell Signaling Technology (1 mentions) Anti plcγ1, by Cell Signaling Technology (1 mentions) Anti slp 76, by Cell Signaling Technology (1 mentions) Anti lat, by Merck Group (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti lck, by Cell Signaling Technology (1 mentions) Anti cd150, by BioLegend (1 mentions) Anti slp76py128, by BD (1 mentions) Anti il 12 rβ2, by R&D Systems (1 mentions) Anti il 12rβ1, by BD (1 mentions) Anti il 12 rβ2 305719, by R&D Systems (1 mentions) Anti sos1, by Santa Cruz Biotechnology (1 mentions) Anti cd3ζ 6b10.2, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

PY783 PLC-γ1 (2 citations)

2 protocols using anti plc γ1 py783

Comprehensive Immunoblotting and Flow Cytometry Antibodies

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The following antibodies were used for immunoblotting, cell-surface, and intracellular stains: The anti-LAT Y191 and anti-LAT from Millipore. The anti-LCK pY505, and anti-SLP-76 pY128, anti-IL-12Rβ1, and annexin V from BD Biosciences. The anti-PLC-γ1 pY783, anti-p38 pT180/Y182, anti-AKT pT308, anti-ZAP-70 pY319, anti-SRC pY416, anti-PLC-γ1, anti-LCK, anti-SLP-76, anti-AKT, anti-STAT4, anti-STAT4 pY693, anti-CD25, and anti-ERK 1/2 antibodies were purchased from Cell Signaling Technologies. The anti ERK1/2 pTpY185/187 and anti-AKT pS473 were from Invitrogen. The anti-GAPDH was from Meridian Life Science. The DyLight 800 and DyLight 680 labeled secondary antibodies were obtained from Thermo Scientific. The FITC anti-IFN-γ (4S.B3), Alexa Fluor 647 anti-IL-10 (JES3-9D7), Alexa Fluor 647 anti-IL-4 (8D4-8), Alexa Fluor 647 anti-TNF-α (Mab11), and APC anti-IL-13 (JES10-5A2) all from BioLegend. The anti-CD3 (OKT3), anti-CD4 (RPA-T4), FITC anti-CD28 (CD28.2), anti-CD-2 (RPA-2.10), anti-CD49d (9F10), anti-CD11a (HI111), FITC anti-CD278, anti-CD150, and DyLight 488 IgG (Ply4053) antibodies and propidium iodine were obtained from Biolegend. Anti-IL-12 Rβ2 was purchased from R&D Systems.

Vacaflores A., Chapman N.M., Harty J.T., Richer M.J, & Houtman J.C. (2016). Exposure of Human CD4 T Cells to IL-12 Results in Enhanced TCR-Induced Cytokine Production, Altered TCR Signaling, and Increased Oxidative Metabolism. PLoS ONE, 11(6), e0157175.

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Antibody Immunoblotting and Staining Protocol

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Antibodies used for immunoblotting, cell-surface, and intracellular stains were purchased from commercial sources. The anti-LAT Y191 (C305) was from Millipore. The anti-LCK pY505 (4/Lck (pY505), anti-SLP-76 pY128 (J141-668.36.58), and anti-IL-12Rβ1 (114) were from BD Biosciences. The anti-PLC-γ1 pY783 (polyclonal), anti-p38 pT180/Y182 (3D7), anti-p38 (polyclonal), anti-AKT pT308 (244F9), anti-ZAP-70 pY319 (polyclonal), anti-SRC pY416 (polyclonal), anti-STAT4 (2A2), anti-JNK T183/Y185 (polyclonal), anti-MKK3/MKK6 pS189/S207 (D8E9), and anti-STAT4 pY693 (D2E4) antibodies were purchased from Cell Signaling Technologies. The anti-ERK1/2 pTpY185/187 (polyclonal) was from Invitrogen. The anti-SOS1 (polyclonal) and anti-CD3-ζ (6B10.2) from Santa Cruz Biotechnology. The anti-GAPDH was from Meridian Life Science. The DyLight 800 and DyLight 680 labeled secondary antibodies were obtained from Thermo Scientific. The FITC anti-IFN-γ (4S.B3), APC anti-TNF-α (Mab11), anti-CD3 (OKT3), anti-CD28 (CD28.2), anti-CD2 (RPA-2.10), anti-CD49d (9F10), anti-CD11a (HI111) from BioLegend. Anti-IL-12 Rβ2 (305719) was purchased from R&D Systems. The anti-mouse IgG was from Southern Biotech.

Vacaflores A., Freedman S.N., Chapman N.M, & Houtman J.C. (2016). Pretreatment of activated human CD8 T cells with IL-12 leads to enhanced TCR-induced signaling and cytokine production. Molecular immunology, 81, 1-15.

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