At the end of drug treatment, 3 ml blood samples were collected from the heart of animals under 3.6% chloral hydrate (360 mg/kg) intraperitoneal anesthesia (n=4 rats/group) and the rats were sacrificed by decapitation. Blood samples were coagulated for ~25 min at room temperature, then centrifuged at 400 × g for 20 min at room temperature. Hitachi 7600 biochemical analyzer (Hitachi, Ltd.) was used to evaluate blood glucose and lipids including endothelin (ET), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c) and high-density lipoprotein cholesterol (HDL-c). In addition, oxidative stress detection kits purchased from Nanjing Jiancheng Bioengineering Institute (cat. nos. A001-3, A003-8 and A006-2) were used to detect superoxide dismutase (SOD), total oxidant status (TOS) and malondialdehyde (MDA) in serum samples according to the manufacturer's instructions, respectively. All above serum indicators were evaluated in samples from each rat with at least three repeats.
Oxidative stress detection kits
Manufactured by Nanjing Jiancheng
Sourced in China, United States
Oxidative stress detection kits are laboratory equipment designed to measure and analyze the levels of oxidative stress in biological samples. These kits provide a standardized and reliable method for the quantification of various biomarkers associated with oxidative stress, such as reactive oxygen species, antioxidants, and oxidative damage markers.
Automatically generated - may contain errors
Lab products found in correlation
7600 biochemical analyzer, by Hitachi (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Oil red o stain, by Merck Group (1 mentions)
Rapamycin, by AG Scientific (1 mentions)
Coomassie blue g 250, by Nanjing Jiancheng (1 mentions)
4 protocols using oxidative stress detection kits
1
Evaluating Blood Markers in Rat Drug Study
2
Oxidative Stress Evaluation in ALI
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A supernatant was obtained for biochemical measurement of intestinal tissues after homogenization and centrifugation in cold PBS, and its protein concentration was detected according to the manufacturer’s instructions. The activity of glutathione (GSH), myeloperoxidase (MPO), superoxide dismutase (SOD) and malondialdehyde (MDA) in gut tissues were measured to evaluate the degree of oxidative stress damage in ALI mice. Oxidative stress detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
3
Evaluating Rapamycin's Impact on Oxidative Stress
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FA was supplied by Sigma‐Aldrich (St Louis, MO., USA); rapamycin was supplied by AG Scientific, Inc. (San Diego, CA., USA); ELISA kits were purchased from R&D systems (Minneapolis, MN., USA); oxidative stress detection kits were purchased from Jiancheng Bioengineering Institute (NanJing, China); oil red O stain was obtained from Sigma‐Aldrich; Lillie‐Mayer's haematoxylin and eosin (H&E) stain was obtained from Cosmo Bio Company (Tokyo, Japan); Dulbecco's modified Eagle's medium (DMEM, Gibco, USA), cell lysis buffer (Cell signalling technology, USA) and antibodies against α‐smooth muscle actin (SMA), OPN, mTOR, phosphorylated (p)‐mTOR, p70S6K and phosphorylated (p)‐p70S6K were obtained from Cell Signaling (Danvers, MA., USA).
4
Biomarkers of Metabolic Dysfunction
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The levels of serum albumin, total protein, serum creatinine and blood urea nitrogen, TAG, total cholesterol, LDL, HDL, fasting blood glucose, insulin and homoeostasis model assessment of insulin resistance (HOMA-IR) were determined by automatic clinical chemistry analyzer (Modular Corporation). Coomassie Blue G-250 (Nanjing Jiancheng Bioengineering Institute) was used to detect 24-h proteinuria in the rats. The levels of malondialdehyde (MDA), protein carbonylation, GSH peroxidase (GSH-PX) and superoxide dismutase (SOD) in serum were detected using oxidative stress detection kits (Nanjing Jiancheng Bioengineering Institute). Levels of AngII in the renal cortex were detected using RIA kits (provided by Shanghai Chinese Traditional Medical University). ELISA kits (R&D Systems) were used to measure the levels of AngII in serum and supernatant of cultured cells. All of the samples for measuring AngII were pre-treated by passing them through a Centricon-10 column with a cut-off of >10 000 Da (Amicon; EMD Millipore). The amount of fibronectin (FN) and TGF-β1 released in the cell culture medium was also detected using ELISA kits.
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