Primary myoblasts were cultured in 60 mm plates, then transfected with different vectors or siRNAs. The different treatment cells were washed three times with PBS and collected for FITC-AnnexinV/PI staining assay. Based on the protocol of Cell Apoptosis Detection Kit (BestBio, Shanghai, China), the cells were washed twice with PBS and then resuspended in 400 μL 1 × Annexin V at a density of 1 × 106 cells/mL. Cells were incubated with 5 μL Annexin V-FITC at 2°C–8°C in the dark for 15 min and then for 5 more min upon addition of 10 μL PI. Stained cells were immediately analyzed by flow cytometry (FACS Canto II, BD BioSciences, USA).
Cell apoptosis detection kit
Manufactured by BestBio
Sourced in China
The Cell Apoptosis Detection Kit is a laboratory equipment designed to detect and quantify apoptosis, a programmed cell death process, in cell samples. The kit provides the necessary reagents and protocols to perform apoptosis assays, allowing researchers to analyze and measure the extent of cell death within a given cell population.
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3 protocols using cell apoptosis detection kit
1
Apoptosis Detection in Transfected Myoblasts
2
Cell Apoptosis Assessment by Flow Cytometry
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The cell apoptosis was tested by cell apoptosis detection kit (BestBio, Shanghai, China). Cells were digested into single cells with trypsin (without EDTA) and washed twice with ice-cold PBS. Annexin V staining was carried out using the cell apoptosis detection kit according to the manufacturer's instructions. After 15 min incubation in a dark at 4℃ and then added PI staining gently mix and incubate at 4℃ for 5min, the cells were immediately analyzed by flow cytometer.
3
Cell Cycle and Apoptosis Analysis
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Transfected cells were treated by the Cell Cycle Testing Kit and Cell Apoptosis Detection Kit (BestBio, China) according to the manufacturer's protocol, then cells were immediately measured by a ow cytometer (Beckman, USA).
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