Rabbit anti mouse igg secondary antibody

Manufactured by Jackson ImmunoResearch

Rabbit anti-mouse IgG secondary antibody is a laboratory reagent used to detect the presence of mouse immunoglobulin G (IgG) in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. This antibody is produced by immunizing rabbits with mouse IgG and purifying the resulting antibodies.

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Lab products found in correlation

Anti yfp antibody, by Takara Bio (2 mentions) Anti pa antibody, by Jackson ImmunoResearch (2 mentions) Prc1 cpy antibody, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (2 mentions) Stains all, by Merck Group (2 mentions) Anti lubricin, by Abcam (2 mentions) Seakem gold agarose, by Lonza (2 mentions) Streptomyces hyaluronidase, by Seikagaku (2 mentions) Hybond p polyvinylidene difluoride pvdf membrane, by GE Healthcare (1 mentions)

4 protocols using rabbit anti mouse igg secondary antibody

Antibody Immunodetection Techniques

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Anti-YFP antibody, which detects GFP, was from Clontech (632381). Anti-PA antibody was from Jackson Immuno Research (323-005-024). Anti-Dpm1 antibody was from Molecular Probes/Fisher (A6429). Anti-Pgk1 antiserum is a generous gift from Dr. Jeremy Thorner (University of California, Berkeley). Prc1/CPY antibody was from Invitrogen (A-6428). Antibodies to Ape1 were described previously [51 (link)]. Goat anti-rabbit IgG secondary antibody (Fisher, ICN55676) and rabbit anti-mouse IgG secondary antibody (Jackson, 315-035-003) were from commercial sources.

Lei Y., Wen X, & Klionsky D.J. (2022). Vps13 is Required for Efficient Autophagy in Saccharomyces cerevisiae. Contact, 5, 25152564221136388.

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Protein Detection Antibodies Utilized

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Anti-YFP antibody, which detects GFP, was from Clontech (632381). Anti-PA antibody was
from Jackson Immuno Research (323-005-024). Anti-Dpm1 antibody was from Molecular
Probes/Fisher (A6429). Anti-Pgk1 antiserum is a generous gift from Dr. Jeremy Thorner
(University of California, Berkeley). Prc1/CPY antibody was from Invitrogen (A-6428).
Antibodies to Ape1 were described previously (Klionsky et al., 1992). Goat anti-rabbit IgG
secondary antibody (Fisher, ICN55676) and rabbit anti-mouse IgG secondary antibody
(Jackson, 315-035-003) were from commercial sources.

Lei Y., Wen X, & Klionsky D.J. (2022). Vps13 is Required for Efficient Autophagy in Saccharomyces cerevisiae. Contact, 5, 25152564221136388.

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Reagents for Lubrication Testing

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Reagents for lubrication testing materials were as described previously,^{2 (link),6 (link),33 (link)} including hyaluronan (HA) in a 4,000 kDa form (Healon®, Advanced Medical Optics, Santa Ana, CA). The antibody to PRG4 was anti-Lubricin from AbCam (Cambridge, MA);^{2 (link)} nonspecific rabbit IgG was from Pierce (Rockford, IL); mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch (West Grove, PA). Streptomyces hyaluronidase was from Seikagaku (Tokyo, Japan). SeaKem® gold agarose was from Lonza (Rockland, ME); 50X TAE (2M Tris, 0.5M EDTA) electrophoresis buffer was from Life Technologies (Carlsbad, CA); Hybond™-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare (Piscataway, NJ). Stains-All was from Sigma-Aldrich (St. Louis, MO).

Grissom M.J., Temple-Wong M.M., Adams M.S., Tom M., Schumacher B.L., McIlwraith C.W., Goodrich L.R., Chu C.R, & Sah R.L. (2014). Synovial Fluid Lubricant Properties Are Transiently Deficient After Arthroscopic Articular Cartilage Defect Repair With Platelet-Enriched Fibrin Alone and With Mesenchymal Stem Cells. Orthopaedic Journal of Sports Medicine, 2(7), 2325967114542580.

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Lubrication Testing Materials Protocol

Cited 1 time

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Reagents for lubrication testing materials were as described previously,^{2 (link),6 (link),33 (link)} including HA in a 4000-kDa form (Healon; Advanced Medical Optics). The antibody to PRG4 was anti-Lubricin from AbCam,^{2 (link)} nonspecific rabbit IgG was from Pierce, and mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch. Streptomyceshyaluronidase was from Seikagaku, SeaKem gold agarose was from Lonza; 50X TAE (2M Tris, 0.5 M ethylenediamine tetraacetic acid [EDTA]) electrophoresis buffer was from Life Technologies, Hybond-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare, and Stains-All was from Sigma-Aldrich.

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