Tween 80

Manufactured by MP Biomedicals

Sourced in United States, France, Japan

Tween 80 is a non-ionic surfactant commonly used in laboratory settings. It is a clear, viscous liquid with a slightly sweet odor. Tween 80 is primarily used as an emulsifier, dispersing agent, and solubilizer in various scientific applications.

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Lab products found in correlation

Bovine serum albumin (bsa), by Fujifilm (3 mentions) Middlebrook 7h9 broth, by BD (3 mentions) Sodium chloride (nacl), by Fujifilm (2 mentions) Mycobacteria 7h11 agar, by BD (2 mentions) Fibrinogen, by Merck Group (1 mentions) Thrombin, by Merck Group (1 mentions) Cacl2, by Fujifilm (1 mentions) Spectramax paradigm, by Molecular Devices (1 mentions) 96 well plate, by Corning (1 mentions) Pai 1, by ProSpec (1 mentions) Sorbitol, by Fujifilm (1 mentions) Nourseothricin, by Jena Biosciences (1 mentions) Menadione, by Merck Group (1 mentions) Potassium chloride (kcl), by Fujifilm (1 mentions) D glucose, by Fujifilm (1 mentions) Dithiothreitol (dtt), by Fujifilm (1 mentions) Hygromycin b, by Tokyo Chemical Industry (1 mentions) Caffeine, by Tokyo Chemical Industry (1 mentions) Tunicamycin, by Cayman Chemical (1 mentions) Congo red, by Merck Group (1 mentions)

13 protocols using tween 80

Turbidity Assay for Fibrin Gel Formation and Lysis

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The turbidity assay was performed in quadruplicate according to a minor modification of the method of Kim et al.34 (link) at 37 °C in a 96-well plate (Corning, Corning, NY, USA) using a SpectraMax Paradigm (Molecular Devices, Sunnyvale, CA, USA) as a plate reader. Turbidity was monitored once per minute at a 350 nm wavelength and was calculated as the mean value (n = 4) in a volume of 100 μl HBS at pH 7.4.
For fibrin gel formation, 2.0 mg/ml fibrinogen (Sigma, St. Louis, MO, USA); 5 mM CaCl₂ (Wako Pure Chemical); 0.01% Tween 80 (MP Biomedical, Santa Ana, CA, USA); and 0.5 NIH unit/ml thrombin (Sigma) were added to 10 μg/ml Fab fragments or 0.3 unit/ml antithrombin (SLS Behring K. K., King of Prussia, PA, USA) as AT III.
For lysis of the fibrin gel, 2.0 mg/ml fibrinogen (Sigma); 5 mM CaCl₂ (Wako Pure Chemical); 0.01% Tween 80 (MP Biomedical); 0.5 NIH unit/ml thrombin (Sigma); 0.2 μM PLG (Enzyme Research Laboratories, South Bend, IN, USA); and 0.3 nM tPA (Technoclone, Vienna, Austria) were added to 10 μg/ml Fab fragments or to a mixture of 0.10 μM α₂-PI (Hematologic Technologies, Essex Junction, VT, USA) and 2.0 ng/ml PAI-1 (Prospec, East Brunswick, NJ, USA) as a negative control.

Obonai T., Fuchigami H., Furuya F., Kozuka N., Yasunaga M, & Matsumura Y. (2016). Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment. Scientific Reports, 6, 23613.

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Microbial Susceptibility to Clarithromycin

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The MIC of clarithromycin was measured by inoculating the bacteria in Middlebrook 7H9 medium (Difco Laboratories, Detroit, MI) containing 0.2% glycerol, 0.1% Tween 80 (MP Biomedicals, Illkirch, France) and 10% Middlebrook albumin-dextrose-catalase (7H9/ADC/Tween 80) (OD₆₀₀ 0.003) in 96-well plates followed by incubation at 37 °C for 2 weeks.

Tateishi Y., Ozeki Y., Nishiyama A., Miki M., Maekura R., Kida H, & Matsumoto S. (2023). Virulence of Mycobacterium intracellulare clinical strains in a mouse model of lung infection – role of neutrophilic inflammation in disease severity. BMC Microbiology, 23, 94.

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Antifungal and Stress Resistance Assay Protocol

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Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Cefotaxime sodium, D-glucose, agar, NaCl, KCl, sorbitol, H₂O₂, DTT, MMS, and AMPH-B were purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). Hygromycin B, caffeine, FLCZ, VRCZ, and N-phenylthiourea were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Congo Red and menadione were purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). Hipolypeptone was purchased from Nihon Pharmaceutical Co., Ltd. (Tokyo, Japan). Tunicamycin (TM) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Sodium dodecyl sulfate (SDS) was purchased from Nippon Gene Co., Ltd. (Tokyo, Japan). Tween 80 was purchased from MP Biomedicals LLC (Santa Ana, MO, USA).

Matsumoto Y., Sugiyama Y., Nagamachi T., Yoshikawa A, & Sugita T. (2023). Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii. Scientific Reports, 13, 13539.

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Microbial Production of Nitrohumic Acid

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Nitrohumic acid and 2-PAP were purchased from Tokyo Kasei (Tokyo, Japan). 2-PPE was purchased from Combi-Blocks (San Diego, CA, USA). MPHPV was purchased from Fujifilm Wako Chemicals (Osaka, Japan). Bacto yeast extract was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Tween 80 was purchased from MP Biomedicals (Illkirch, France). All other chemicals were of analytical grade. Basal medium contained (per liter) KCl (1.71 g), Na₂HPO₄ (0.50 g), MgSO₄·7H₂O (0.05 g), CaCO₃ (0.02 g), FeSO₄·7H₂O (0.01 g), and (NH₄)₂SO₄ (1.50 g) (pH 7.2).

Oya S., Tonegawa S., Nakagawa H., Habe H, & Furuya T. (2022). Isolation and characterization of microorganisms capable of cleaving the ether bond of 2-phenoxyacetophenone. Scientific Reports, 12, 2874.

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Pharmacokinetic Evaluation of MR1704

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MR1704 was dissolved in N, N‐dimethylacetamide (Wako)/polyethylene glycol 400 (Wako)/water (4/4/2, v/v/v) for intravenous administration, and suspended in 1% Tween^® 80 (MP Biochemicals, Santa Ana, CA) containing 0.5% carboxymethyl cellulose (Nacalai, Kyoto, Japan) for oral administration. MR1704 was administered to male Sprague‐Dawley rats (7 weeks old) at a dose of 3 mg kg⁻¹ in 1 mL intravenously or at doses of 0.3, 1, 3, and 10 mg kg⁻¹ in 10 mL orally. The rats used for oral administration were fasted overnight before the experiment. Pharmacokinetic parameters were calculated, using the analysis program (WinNonlin noncompartmental analysis program, version 6.1; Pharsight, Cary, NC).

Tsuda N., Kawaji A., Sato T., Takagi M., Higashi C., Kato Y., Ogawa K., Naba H., Ohkouchi M., Nakamura M., Hosaka Y, & Sakaki J. (2017). A novel free fatty acid receptor 1 (GPR40/FFAR1) agonist, MR1704, enhances glucose‐dependent insulin secretion and improves glucose homeostasis in rats. Pharmacology Research & Perspectives, 5(4), e00340.

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Mycobacteria Growth and Antibiotic Susceptibility

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All mycobacteria strains were grown in Middlebrook 7H9 broth (BD, Franklin Lakes, NJ) supplemented with 0.2% (v/v) glycerol, 0.05% (v/v) Tween 80 (MP Biomedicals, Santa Ana, CA), and 10% ADC enrichment (5% bovine serum albumin [Wako Pure Chemical Industries, Osaka, Japan], 0.81% NaCl, and 2% D-glucose) (7H9-ADC broth) or on Mycobacteria 7H11 agar (BD) supplemented with 0.5% (v/v) glycerol and 10% OADC enrichment (ADC enrichment supplemented with 0.06% [v/v] oleic acid) (7H11-OADC agar). For biofilm formation experiment mycobacteria strains were grown on liquid.
Hygromycin B (HYG), kanamycin sulfate (KAN), rifampicin (RMP), amikacin (AMK,) and clarithromycin (CLA) were purchased from Wako Pure Chemical Industries [Osaka, Japan]; and INH from SIGMA-ALDRICH [St. Louis, USA]. AMK stock solutions were prepared in water. Stock solutions of all other compounds were prepared in 100% dimethyl sulfoxide (DMSO) and then filter sterilized (pore size 0.45 µm). These components were frozen in aliquots at − 20 °C.
CV-AM and SG were purchased from Invitrogen (Life-Technologies Corporation, California, USA). CV-AM was dissolved in 250 µl (μl) of DMSO. SG was diluted from the manufacture’s 5 mM stock solution to a final concentration of 50 μM by DMSO. The dyes were stored at − 30 °C.
PMAxx dye was purchased from Cosmo Bio Co., LTD (Tokyo, Japan).

Ilinov A., Nishiyama A., Namba H., Fukushima Y., Takihara H., Nakajima C., Savitskaya A., Gebretsadik G., Hakamata M., Ozeki Y., Tateishi Y., Okuda S., Suzuki Y., Vinnik Y.S, & Matsumoto S. (2021). Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance. Scientific Reports, 11, 10953.

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Cultivation of M. avium Strain MAH 104

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The M. avium (MAH 104) strain was grown in Middlebrook 7H9 broth (Difco), supplemented with 0.2% glycerol, ADC, and 0.05% Tween 80 (MP Biomedical, LLC (Solon, OH, USA). When M. avium grows to OD₆₀₀ = 0.5, this is considered as the exponential phase and when it grows to OD₆₀₀ = 2.8, this is considered to be the stationary phase. Each measurement was detected from three biological and three technical replicates.

Enany S., Ato M, & Matsumoto S. (2021). Differential Protein Expression in Exponential and Stationary Growth Phases of Mycobacterium avium subsp. hominissuis 104. Molecules, 26(2), 305.

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Mycobacterium smegmatis Culturing Protocols

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Δmdp1 was kindly provided by Dr. John L. Dahl (University of Minnesota Duluth)^{26 (link)}. E. coli DH5α strain was used for all gene manipulation in this study. All M. smegmatis strains were grown in Middlebrook 7H9 broth (BD, Franklin Lakes, NJ) supplemented with 0.2% (v/v) glycerol, 0.05% (v/v) Tween 80 (MP Biomedicals, Santa Ana, CA), and 10% ADC enrichment (5% bovine serum albumin [Wako Pure Chemical Industries, Osaka, Japan], 0.81% NaCl, and 2% D-glucose) (7H9-ADC broth) or on Mycobacteria 7H11 agar (BD) supplemented with 0.5% (v/v) glycerol and 10% OADC enrichment (ADC enrichment supplemented with 0.06% [v/v] oleic acid) (7H11-OADC agar). Appropriate antibiotics were also added to the media to maintain the specific genotypes of each strain. All E. coli strains were cultured in LB broth or on LB agar (both from Sigma-Aldrich, St. Louis, MO). Hygromycin B (Hyg), kanamycin (Km), and RIF were purchased from Wako Pure Chemical Industries (Osaka, Japan). INH and Ace were purchased from Sigma Aldrich. ATc was purchased from Cayman Chemical (Ann Arbor, MI).

Savitskaya A., Nishiyama A., Yamaguchi T., Tateishi Y., Ozeki Y., Nameta M., Kon T., Kaboso S.A., Ohara N., Peryanova O.V, & Matsumoto S. (2018). C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein. Scientific Reports, 8, 8197.

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Curcumin Extraction from Turmeric Rhizomes

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Curcuma longa dried rhizomes were purchased from a local herbalist from which the Curcumin was extracted. Absolute Ethanol, Span® 60, and HPMC E50 were supplied by Loba-Chemie, Mumbai, India. Tween® 80 was supplied by MP Biomedicals, USA. All the other stuff was analytical grade and used what they were given. Curcumin Standard; 500 mg, Sigma Aldrich C7727.

El Hosary R., Teaima M.H., El-Nabarawi M., Yousry Y., Eltahan M., Bakr A., Aboelela H., Abdelmonem R, & Nassif R.M. (2023). Topical delivery of extracted curcumin as curcumin loaded spanlastics anti-aging gel: Optimization using experimental design and ex-vivo evaluation. Saudi Pharmaceutical Journal : SPJ, 32(1), 101912.

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Mycobacterium smegmatis Growth Conditions

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M. smegmatis mc²155, M. smegmatis mc²155Δhlp::pMV306^KM, and M. smegmatis mc²155 Δhlp::pMV306^KM/hlp strains were used in this study^{23 (link), 50 (link)}. Cultures were grown to different ODs according to the experiment, i.e., OD₆₀₀ = 0.5, 0.8, 1.0, and 1.2, in Middlebrook 7H9 broth (Difco) enriched with 0.2% (v/v) glycerol and 0.05% Tween 80 (MP Biomedical). Fifty micrograms per milliliter of hygromycin and 10 µg/ml of kanamycin were added to mutant and complemented mutant strain cultures^{50 (link)}.

Enany S., Yoshida Y., Tateishi Y., Ozeki Y., Nishiyama A., Savitskaya A., Yamaguchi T., Ohara Y., Yamamoto T., Ato M, & Matsumoto S. (2017). Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions. Scientific Reports, 7, 6810.

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