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Typhoon fla 7000 control software version 1

Manufactured by GE Healthcare
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The Typhoon FLA 7000 control software Version 1.3 is a software package developed by GE Healthcare to operate the Typhoon FLA 7000 imaging system. The software's core function is to provide a user interface for controlling the imaging system's various features and settings.

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Lab products found in correlation

Megaprime dna labeling system, by GE Healthcare (2 mentions) Ultrahyb hybridization buffer, by Thermo Fisher Scientific (2 mentions) Fla7000ip typhoon storage phosphorimager, by GE Healthcare (2 mentions) α 32p dctp, by PerkinElmer (2 mentions) Turboblotter, by Cytiva (2 mentions)

2 protocols using typhoon fla 7000 control software version 1

1

Northern Blot Analysis of RNA Transcripts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Northern analysis was performed as previously described (Gruner et al., 2016 (link)) using P32 labeled DNA probes. Briefly, Glyoxal-DMSO was used to denature RNA and electrophoresis was performed using 1% BPTE agarose gels. Transfer to Nylon membrane was performed using Turboblotter (Whatman). After transfer and washing, blot was crosslinked using a UV Stratalinker 1800 (Stratagene). DNA probes were prepared using Megaprime DNA labeling system (GE Healthcare, RPN1606) labeled with α32-P dCTP (Perkin Elmer). Probing was performed in a hybridization oven overnight at 45–55°C using ULTRAhyb hybridization buffer (ThermoFisher, AM8669), followed by 3–4 washes at 50–60°C and exposure on phosphoscreen. Image acquisition was performed using a FLA7000IP Typhoon Storage Phosphorimager using Typhoon FLA 7000 control software Version 1.3 (GE Healthcare).
Zhang Z., So K., Peterson R., Bauer M., Ng H., Zhang Y., Kim J.H., Kidd T, & Miura P. (2019). Elav-Mediated Exon Skipping and Alternative Polyadenylation of the Dscam1 Gene Are Required for Axon Outgrowth. Cell reports, 27(13), 3808-3817.e7.
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2

Northern Blot Analysis of RNA Transcripts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Northern analysis was performed as previously described (Gruner et al., 2016 (link)) using P32 labeled DNA probes. Briefly, Glyoxal-DMSO was used to denature RNA and electrophoresis was performed using 1% BPTE agarose gels. Transfer to Nylon membrane was performed using Turboblotter (Whatman). After transfer and washing, blot was crosslinked using a UV Stratalinker 1800 (Stratagene). DNA probes were prepared using Megaprime DNA labeling system (GE Healthcare, RPN1606) labeled with α32-P dCTP (Perkin Elmer). Probing was performed in a hybridization oven overnight at 45–55°C using ULTRAhyb hybridization buffer (ThermoFisher, AM8669), followed by 3–4 washes at 50–60°C and exposure on phosphoscreen. Image acquisition was performed using a FLA7000IP Typhoon Storage Phosphorimager using Typhoon FLA 7000 control software Version 1.3 (GE Healthcare).
Zhang Z., So K., Peterson R., Bauer M., Ng H., Zhang Y., Kim J.H., Kidd T, & Miura P. (2019). Elav-Mediated Exon Skipping and Alternative Polyadenylation of the Dscam1 Gene Are Required for Axon Outgrowth. Cell reports, 27(13), 3808-3817.e7.
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