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α methylglucoside

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α-methylglucoside is a chemical compound used in various laboratory applications. It functions as a glucoside, serving as a substrate or reagent in biochemical and analytical procedures.

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Lab products found in correlation

Bradford assay kit, by Bio-Rad (2 mentions) Hplc grade acetonitrile, by Merck Group (2 mentions) Bradford reagent, by Merck Group (2 mentions) Low protein binding tube, by Eppendorf (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions) Low binding tips, by Thermo Fisher Scientific (2 mentions) α methylmannoside, by Merck Group (2 mentions) Sequencing grade modified trypsin enzyme, by Promega (2 mentions) Soluble starch, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Ethylene glycol, by Merck Group (1 mentions) Benzyl alcohol, by Merck Group (1 mentions) Choline chloride, by Merck Group (1 mentions) Levulinic acid, by Merck Group (1 mentions) Malonic acid, by Merck Group (1 mentions) 1 3 propanediol, by Merck Group (1 mentions) Maltotriose, by Merck Group (1 mentions)

3 protocols using α methylglucoside

1

Enzymatic Starch Hydrolysis via Glucoamylase

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Glucoamylase from A<.spergillus niger, choline chloride, soluble starch, maltotriose, urea, methyl urea, malonic acid, levulinic acid, ethylene glycol, D-isosorbide, MPh3PBr, glycerol, benzyl alcohol, 1,3-propanediol, glucose, sucrose, α-methyl-glucoside, and methanol were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Miranda-Molina A., Xolalpa W., Strompen S., Arreola-Barroso R., Olvera L., López-Munguía A., Castillo E, & Saab-Rincon G. (2019). Deep Eutectic Solvents as New Reaction Media to Produce Alkyl-Glycosides Using Alpha-Amylase from Thermotoga maritima. International Journal of Molecular Sciences, 20(21), 5439.
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2

Glycoprotein Sample Preparation and Analysis

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Trifluoroacetic acid (TFA), tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide (IAA), α-methylmannoside, α-methylglucoside, TEAB, Bradford reagents, water (HPLC grade), formic acid (HPLC grade), and acetonitrile (HPLC grade) were purchased from Sigma-Aldrich (St. Louis, MO). The Pierce centrifuge column (2 mL), C18 spin column, and low binding tips were purchased from Thermo Fisher Scientific (Fair Lawn, NJ). Low protein binding tubes were purchased from Eppendorf (Hauppauge, NY). Agarose bound Lens culinaris agglutinin (LCA) was purchased from Vector Laboratories (Burlingame, CA). Amicon Ultra 3K centrifugal filters (15 and 4 mL) were purchased from Millipore (Billerica, MA). The Bradford assay kit was purchased from BioRad (Hercules, CA). Isobaric tags for relative and absolute quantitation (iTRAQ) 4-plex reagent kits were purchased from AB Sciex (Framingham, MA). Sequencing grade modified trypsin enzyme was obtained from Promega (Madison, WI). Endoglycosidase F3 (Endo F3) was purchased from BA-Bio (San Mateo, CA).
Tan Z., Yin H., Nie S., Lin Z., Zhu J., Ruffin M.T., Anderson M.A., Simeone D.M, & Lubman D.M. (2015). Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of proteome research, 14(4), 1968-1978.
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3

Glycoproteomic Analysis Workflow

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Trifluoroacetic acid, tris
(2-carboxyethyl) phosphine (TCEP), iodoacetamide (IAA), α-methylmannoside,
α-methylglucoside, TEAB, Bradford reagents, water [high-performance
liquid chromatography (HPLC) grade], formic acid (FA) (HPLC grade),
and acetonitrile (HPLC grade) were purchased from Sigma-Aldrich (St.
Louis, MO). The Pierce centrifuge column (2 mL), centrifuge filters
(0.45 μm), C18 spin column, and low binding tips were purchased
from Thermo Fisher Scientific (Fair Lawn, NJ). Low protein-binding
tubes were purchased from Eppendorf (Hauppauge, NY). Agarose-bound Lens culinaris agglutinin (LCA) was purchased from
Vector Laboratories (Burlingame, CA). Amicon ULtra 3 K (0.5 and 4.0
mL) and 10 k (4.0 mL) centrifugal filters were purchased from Millipore
(Billerica, MA). The Bradford assay kit was purchased from BioRad
(Hercules, CA). iTRAQ 8-plex reagent kits were purchased from AB Sciex
(Redwood, CA). Sequencing-grade modified trypsin enzyme was obtained
from Promega (Madison, WI). Endoglycosidase F3 (Endo F3) was purchased
from BA-Bio (San Mateo, CA). Human 14 multiple affinity removal system
spin cartridges for the depletion of high-abundant proteins from human
proteomic samples were from Agilent (Santa Clara, CA).
Tan Y., Zhu J., Gutierrez Reyes C.D., Lin Y., Tan Z., Wu Z., Zhang J., Cano A., Verschleisser S., Mechref Y., Singal A.G., Parikh N.D, & Lubman D.M. (2023). Discovery of Core-Fucosylated Glycopeptides as Diagnostic Biomarkers for Early HCC in Patients with NASH Cirrhosis Using LC-HCD-PRM-MS/MS. ACS Omega, 8(13), 12467-12480.
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