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Mycoalert mycoplasma detection assay kit

Manufactured by Lonza
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The MycoAlert mycoplasma detection assay kit is a diagnostic tool used to detect the presence of mycoplasma contamination in cell cultures. The kit provides a quick and reliable method for detecting mycoplasma without the need for specialized equipment or extensive sample preparation.

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Lab products found in correlation

Granta 519 cells, by American Type Culture Collection (2 mentions) Human jurkat t cells clone e6 1, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

MycoAlert Mycoplasma Detection Kit (2023 citations) MycoAlert (576 citations) MycoAlert kit (330 citations) MycoAlert Detection Kit (311 citations) Mycoplasma Detection Kit (90 citations) MycoAlert assay (35 citations) MycoAlert Mycoplasma Detection Assay (32 citations) MycoAlert detection assay (12 citations) MycoAlert Mycoplasma Kit (7 citations) Mycoplasma detection assay (2 citations)

3 protocols using mycoalert mycoplasma detection assay kit

1

Everolimus Treatment of MCL Cell Line

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Granta 519 cells were obtained from the American Type Culture Collection (Manassas, Virginia). This cell line has been previously well characterized as a model of MCL. Cells were tested for mycoplasma contamination using the MycoAlert mycoplasma detection assay kit from Lonza (Basel, Switzerland) and found to be negative. The cell line was verified to be authentic by the University of Arizona Genomics Core. Cells were routinely grown in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (HyClone Thermo Scientific, Waltham, Massachusetts) in a humidified incubator at 37°C and 5% CO2. Per test parameters, the DMEM either had 4.5 or 1.0 g/L of glucose. Cells were treated with 10 nmol/L everolimus dissolved in biological grade 100% dimethyl sulfoxide (DMSO).
Akhenblit P.J., Hanke N.T., Gill A., Persky D.O., Howison C.M., Pagel M.D, & Baker A.F. (2016). Assessing Metabolic Changes in Response to mTOR Inhibition in a Mantle Cell Lymphoma Xenograft Model Using AcidoCEST MRI. Molecular imaging, 15, 1536012116645439.
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2

Cell Line Maintenance and Validation

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GL261 cells were obtained from NCI Division of Cancer Treatment and Diagnosis Tumor Repository. Human Jurkat T cells (clone E6–1) were obtained from ATCC. Cells were maintained in liquid nitrogen in Bambanker cryopreservation media until further use. Cells were tested for Mycoplasma using Lonza MycoAlert Mycoplasma Detection Assay Kit prior to any use. Cells were used within 3–5 passages.
Nisnboym M., Vincze S.R., Xiong Z., Sneiderman C.T., Raphael R.A., Li B., Jaswal A.P., Sever R.E., Day K.E., LaToche J.D., Foley L.M., Karimi H., Hitchens T.K., Agnihotri S., Hu B., Rajasundaram D., Anderson C.J., Blumenthal D.T., Pearce T.M., Uttam S., Nedrow J.R., Panigrahy A., Pollack I.F., Lieberman F.S., Drappatz J., Raphael I., Edwards W.B, & Kohanbash G. (2023). Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma. Cancer Research Communications, 3(7), 1173-1188.
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3

Everolimus Treatment of MCL Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
Granta 519 cells were obtained from the American Type Culture Collection (Manassas, Virginia). This cell line has been previously well characterized as a model of MCL. Cells were tested for mycoplasma contamination using the MycoAlert mycoplasma detection assay kit from Lonza (Basel, Switzerland) and found to be negative. The cell line was verified to be authentic by the University of Arizona Genomics Core. Cells were routinely grown in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (HyClone Thermo Scientific, Waltham, Massachusetts) in a humidified incubator at 37°C and 5% CO2. Per test parameters, the DMEM either had 4.5 or 1.0 g/L of glucose. Cells were treated with 10 nmol/L everolimus dissolved in biological grade 100% dimethyl sulfoxide (DMSO).
Akhenblit P.J., Hanke N.T., Gill A., Persky D.O., Howison C.M., Pagel M.D, & Baker A.F. (2016). Assessing Metabolic Changes in Response to mTOR Inhibition in a Mantle Cell Lymphoma Xenograft Model Using AcidoCEST MRI. Molecular imaging, 15, 1536012116645439.
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+ Expand

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