Cpg odn 1826 tlrl 1826

Manufactured by InvivoGen

Sourced in United States

CpG-ODN 1826 (tlrl-1826) is a synthetic oligodeoxynucleotide (ODN) containing unmethylated CpG motifs. It is designed to activate the Toll-like receptor 9 (TLR9) signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Chloroquine c6628, by Merck Group (1 mentions) Hrp conjugated anti flag a 8592, by Merck Group (1 mentions) Ha tag 3724, by Cell Signaling Technology (1 mentions) Sc 53909, by Santa Cruz Biotechnology (1 mentions) Immunization grade bovine type 2 collagen, by Chondrex (1 mentions) Easysep human pan dc pre enrichment kit, by STEMCELL (1 mentions)

2 protocols using cpg odn 1826 tlrl 1826

Antibody-Based Signaling Pathway Analysis

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Antibodies against phospho-ERK1/2 (4370), phospho-p38 (9215), phospho-JNK1/2 (9251), phospho-IκBα (9246), TAK1 (4505), TAB2 (Leu 330, 3745), SUMO1 (4930), and HA-tag (3724) were obtained from Cell Signaling Technology. Antibodies against ERK1/2 (201245-4A4), p38 (200782), JNK1/2 (201001), IκBα (200517) and TRAF6 (380803) were obtained from Zen Bioscience, China. Anti-β-Actin (A2228), anti-Flag (F1804), and HRP-conjugated anti-Flag (A8592) antibodies were purchased from Sigma-Aldrich. Anti-Myc (MA1-21316), HRP-conjugated anti-Myc (MA1-81357), and HRP-conjugated goat anti-rabbit IgG (31460) antibodies were purchased from Invitrogen. Antibodies against TRAF6 (SC-8409), TAB2 (N48-73, SC-398188), and GST (SC-53909) were obtained from Santa Cruz Biotechnology. The antibodies against RIP1 (610458), ubiquitin (04-263), and K63-linked ubiquitin (BML-PW0600) were purchased from BD Bioscience, Merck Millipore, and Enzo Life Science, respectively. The HRP-conjugated anti-mouse secondary antibody (KCB002) was purchased from Rockland.
LPS (E. coli O127:B8, L3129), MG132 (474790), polybrene (H9268) and chloroquine (C6628) were purchased from Sigma-Aldrich. CpG-ODN 1826 (tlrl-1826) and puromycin (540411) were obtained from InvivoGen and Merck Millipore, respectively. Murine TNFα (AF-315-01A) was purchased from PeproTech.

Gu Z., Chen X., Yang W., Qi Y., Yu H., Wang X., Gong Y., Chen Q., Zhong B., Dai L., Qi S., Zhang Z., Zhang H, & Hu H. (2020). The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response. Cellular and Molecular Immunology, 18(8), 1981-1994.

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Purification and Immunization Protocol

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All flow antibodies were purchased from BD Biosciences and BioLegend of USA. And all ELISA kits were purchased from NEO Biosciences of China. EasySep™ Human Pan-DC Pre-Enrichment Kit for DC purification was purchased from STEMCELL of Canada and the purity of the DC up to 86.6 % in the research (Fig. S1). Immunization Grade Bovine Type II Collagen, immunization Grade Chick Type II Collage, Complete and incomplete Freund's adjuvants were purchased from Chondrex, USA. CpG ODN 1826 (tlrl-1826) and recombinant murine GM-CSF (AF-315-03) were purchased from InvivoGen and PeproTech of USA.

Han J., Li X., Luo X., He J., Huang X., Zhou Q., Han Y., Jie H., Zhuang J., Li Y., Yang F., Zhai Z., Wu S., He Y., Yang B, & Sun E. (2020). The mechanisms of hydroxychloroquine in rheumatoid arthritis treatment: Inhibition of dendritic cell functions via Toll like receptor 9 signaling. Biomedicine & Pharmacotherapy, 132, 110848.

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