Km12sm

Manufactured by Korean Cell Line Bank

The KM12SM is a laboratory equipment used for cell culture applications. It is designed to maintain consistent temperature, humidity, and gas levels to support the growth and proliferation of cell lines. The KM12SM provides a controlled environment to facilitate the in vitro cultivation of various cell types.

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Lab products found in correlation

6 protocols using km12sm

Colon Cancer Cell Line Culture

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Ten colon cancer cell lines (DLD1, HT29, HCT116, HCT15, SW620, LOVO, SW480, KM12C, KM12L4, and KM12SM) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in MEM, DMEM, RPMI1640, or L15 medium (Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Gibco), and maintained in a humidified incubator with 5% CO₂ at 37 °C.

Jang B.G., Kim H.S., Bae J.M., Kim W.H., Hyun C.L, & Kang G.H. (2020). Expression Profile and Prognostic Significance of EPHB3 in Colorectal Cancer. Biomolecules, 10(4), 602.

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Colon Cancer Cell Line Apoptosis Assay

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Human colon cancer cell lines KM12SM, HT29, and HCT116 were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea) and maintained in RPMI 1640 supplemented with 10% fetal bovine serum. The cells were cultured at 100% humidity and 5% CO₂ at 37 °C. The NTRK inhibitors LOXO-101, entrectinib, dovitinib, dovitinib lactate, dovitinib dilactic acid, regorafenib, and crizotinib were purchased from Selleck Chemicals (Houston, TX, USA). We used the annexin V-APC/propidium iodide (PI) apoptosis detection kit (Thermo Fisher Scientific, Rockford, IL, USA).

Sohn S.H., Sul H.J., Kim B., Kim B.J., Kim H.S, & Zang D.Y. (2021). TRK inhibitors block NFKB and induce NRF2 in TRK fusion-positive colon cancer. Journal of Cancer, 12(21), 6356-6362.

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Comprehensive CRC Cell Line Characterization

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Forty-eight human CRC cell lines (SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-479, SNU-503, SNU-769A, SNU-769B, SNU-796, SNU-977, SNU-1033, SNU-1040, SNU-1047, SNU-1181, SNU-1197A, SNU-1235, SNU-1411, SNU-1406, SNU-1544, SNU-1684, SNU-1746, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, Caco-2, COLO201, COLO205, COLO320, DLD1, HCT-8, HCT-15, HCT-116, HT-29, LoVo, LS174T, NCI-H716, SW-403, SW-480, SW-620, SW-1116, WiDr, KM12C, KM12SM, and KM12L4) were obtained from the Korean Cell Line Bank. All cell lines were maintained in RPMI1640 media with 10% fetal bovine serum at 37°C and 5% CO₂. All cell lines were tested and authenticated by Korean Cell Line Bank using DNA finger printing analysis.

Kim S.C., Hong C.W., Jang S.G., Kim Y.A., Yoo B.C., Shin Y.K., Jeong S.Y., Ku J.L, & Park J.G. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Translational Oncology, 11(5), 1232-1243.

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Culturing Colorectal Cancer Cell Lines

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The colorectal cancer cell lines HCT-116, SW1116, LS174T, HT-29, KM12C, KM12SM, SW48, SW480, SW620, DLD-1, LOVO, COLO205 were obtained from the Korean Cell Line Bank (Seoul, Korea) and were maintained in DMEM (Gibco-BRL, Grand Island, NY) supplemented with 10% fetal bovine serum and 100 μg/ml antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin. Cells were maintained at 37°C in a humidified, 5% CO₂/air atmosphere.

Park S.Y., Lee S.J., Cho H.J., Kim T.W., Kim J.T., Kim J.W., Lee C.H., Kim B.Y., Yeom Y.I., Lim J.S., Lee Y, & Lee H.G. (2016). Dehydropeptidase 1 promotes metastasis through regulation of E-cadherin expression in colon cancer. Oncotarget, 7(8), 9501-9512.

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Colon Cancer Cell Lines Cultivation

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Colon cancer cell lines SW480 (KCLB-10228; colon adenocarcinoma), SW620 (KCLB-10227; colorectal carcinoma), HT-29 (KCLB-30038; rectosigmoid colon adenocarcinoma), DLD1 (KCLB-10221; colon adenocarcinoma), Caco2 (KCLB-30037.1; colon adenocarcinoma), LoVo (KCLB-10229; colon adenocarcinoma, KM12C (KCLB-80015; colon carcinoma), and KM12SM (KCLB-80016; colon carcinoma) cells were purchased from the Korean Cell Line Bank (Seoul, Korea). RKO (ATCC CRL-2577; colon carcinoma), HCT116 (ATCC CCL-247; colorectal carcinoma), and LS174T (ATCC CL-188; colorectal adenocarcinoma) cells were purchased from the American Type Culture Collection (ATCC, USA). Each cell line was authenticated through STR profiling from the cell line bank. Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS) (HyClone) and 100 µg/ml antibiotics [100 U/ml penicillin and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.)] at 37°C under an atmosphere of 5% CO₂ in a humidified incubator.

Park S.Y., Kim J.T., Park E.S., Hwang Y.S., Yoon H.R., Baek K.E., Jung H., Yoon S.R., Kim B.Y., Cho H.J, & Lee H.G. (2021). Collapsin response mediator protein 4 enhances the radiosensitivity of colon cancer cells through calcium-mediated cell signaling. Oncology Reports, 45(4), 6.

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Colorectal Cancer Cell Line Cultivation

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Twelve CRC cell lines (DLD1, HT29, SW620, HCT116, LoVo, KM12C, KM12SM, KM12L4, HCT116, LS174T, SW1116, and Colo205) were purchased from the Korean Cell Line Bank (https://cellbank.snu.ac.kr, Seoul, Korea). Cells were cultured in RPMI1640, MEM, DMEM, or L15 medium (Welgene, Daegu, Korea) at 37 °C in a humidified incubator with 5% CO2 according to the guidelines. All culture media contained 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA) and 1% penicillin/streptomycin (Gibco).

Jang B.G., Kim H.S., Bae J.M., Kim W.H., Kim H.U, & Kang G.H. (2020). SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers. Scientific Reports, 10, 14591.

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