Cryptotanshinone (CPT) was purchased from Shanghai Yuanye Biotechnology Co., Ltd, with a purity of 98% (#B21586, Shanghai, China). High-performance liquid chromatography (HPLC) analysis was applied to further confirm the purity of CPT (98%). Imatinib (Genike) was obtained from Nanjing Chia Tai Tianqing Pharmaceutical Co., Ltd. The second-generation tyrosine kinase inhibitors nilotinib was purchased from Aladdin Chemistry Co., Ltd (#N126111, Shanghai, China). Dasatinib was obtained from Shanghai Yuanye Biotechnology Co., Ltd (#S45672, Shanghai, China). Antibodies used in this study were purchased from Cell Signalling Technology (MA, USA), including cleaved caspase-3 (#9664), cleaved PARP (#5625), p-STAT3 (#9145), Bcr-Abl (#3908), p-Bcr-Abl (#3009), p-Src (#2101) and β-Actin (#3700). Antibody for cleaved caspase-9 (Asp353) (#AF5240) was obtained from Affinity Biosciences (Cincinnati, OH, USA), for eIF4E (#610270) was obtained from BD Biosciences (San Diego, CA, USA), and p-eIF4E (ab76256) was purchased from Abcam. The rabbit polyclonal antibody against Ki-67 (PB9026) and mouse monoclonal antibody against PCNA (BM0104) was obtained from Boster Biological Technology Co., Ltd.
Pb9026
Manufactured by Boster Bio
Sourced in China
The PB9026 is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Cryptotanshinone, by Yuanye Bio-Technology (1 mentions)
P eif4e, by Abcam (1 mentions)
P src, by Cell Signaling Technology (1 mentions)
Dasatinib, by Yuanye Bio-Technology (1 mentions)
P bcr abl, by Cell Signaling Technology (1 mentions)
Eif4e, by BD (1 mentions)
Bcr abl, by Cell Signaling Technology (1 mentions)
P stat3, by Cell Signaling Technology (1 mentions)
Bm0104, by Boster Bio (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Bm3895, by Boster Bio (1 mentions)
2 protocols using pb9026
1
Evaluation of Cryptotanshinone in Leukemia
2
Immunohistochemical analysis of tissue samples
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Tumor tissues were maintained in 4% paraformaldehyde for fixation followed by paraffin-embedding. Paraffin-embedded tissues serial sections (4 µm) were obtained, and then dewaxing and dehydration with xylene and hydrated respectively. Sections were incubated with primary antibody (1:200, PB9026, Boster, China) overnight at 4o °C after antigen retrieval and sealing. Afterwards, sections were incubated with horseradish peroxidase-conjugated secondary antibody (1:200, BM3895, Boster, China) at room temperature. Sections were developed with diaminobenzidine (DAB) and hematoxylin. Finally, sections sealed with neutral resin were observed under microscope.
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