Vitronectin vtn n

Manufactured by Thermo Fisher Scientific

Vitronectin (VTN-N) is a glycoprotein that promotes cell attachment and spreading. It is a component of the extracellular matrix and can bind to various cell surface receptors.

Automatically generated - may contain errors

Lab products found in correlation

Essential 8 medium, by Thermo Fisher Scientific (2 mentions) Essential 8 medium e8, by Thermo Fisher Scientific (1 mentions) Revitacell, by Thermo Fisher Scientific (1 mentions) Y 27632, by Fujifilm (1 mentions) Culturesure y 27632, by Fujifilm (1 mentions) Eon microplate spectrophotometer, by Agilent Technologies (1 mentions) Cck 8 kit, by Dojindo Laboratories (1 mentions) Essential 8 media, by Thermo Fisher Scientific (1 mentions) Facsaria 3, by BD (1 mentions) E8 medium, by Thermo Fisher Scientific (1 mentions) Tesr e8 medium, by STEMCELL (1 mentions)

Close spelling variants of this product

Vitronectin Recombinant Human Protein (VTN-N) Truncated recombinant human vitronectin N-terminal fragment (VTN-N) VTN-N Vitronectin-N Vitronectin

7 protocols using vitronectin vtn n

Pluripotent Stem Cell Culture Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

hESCs line WA09 (H9)^{2 (link)} was purchased from WiCell Research Institute (http://www.wicell.org) and hiPSCs line FN2.1 was previously derived from human foreskin fibroblasts^{54 (link)}. All methods were performed by the relevant guidelines and regulations. Ethical approval was received by the local Ethics Committee (Comité de ética en Investigaciones biomédicas del Instituto FLENI) and written informed consent was obtained from the donor before foreskin fibroblast isolation. hPSCs were cultured on Vitronectin (VTN-N, Gibco) (0.5 µg/cm²) coated dishes in combination with fully defined Essential 8 medium (E8, Gibco) to 80–90% confluency. All cell lines were free of Mycoplasma sp. infection, which was tested as previously described^{55 (link)}.

Mucci S., Isaja L., Rodríguez-Varela M.S., Ferriol-Laffouillere S.L., Marazita M., Videla-Richardson G.A., Sevlever G.E., Scassa M.E, & Romorini L. (2022). Acute severe hypoxia induces apoptosis of human pluripotent stem cells by a HIF-1α and P53 independent mechanism. Scientific Reports, 12, 18803.

+ Open protocol

+ Expand

Culturing and Maintaining Human Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The WA09 (H9, RRID:CVCL_9773) female human embryonic stem cell (hESC) line was used to assess differentiation effects of factors listed in Figure 1C. Cells were grown at 37°C, in a humidified environment at 10% O₂ and 5% CO₂. Cells were maintained on tissue culture plates coated with 0.5 ug/cm² Vitronectin (VTN-N; Gibco, A14700) in Essential 8 (E8) Medium (Gibco, A1517001) with daily media exchange, according to manufacturer instructions. Cells were passaged as colonies using 0.5 mM EDTA when they were approximately 80% confluent, at least every 5 days, and media was supplemented overnight with 1X RevitaCell (Gibco, A2644501) after passage. The cell line was authenticated by STR testing before use and karyotype analysis was performed at least every 10 passages (WiCell).

Sears K.E., Gullapalli K., Trivedi D., Mihas A., Bukys M.A, & Jensen J. (2022). Controlling neural territory patterning from pluripotency using a systems developmental biology approach. iScience, 25(4), 104133.

+ Open protocol

+ Expand

Feeder-free Culture of Human Stem Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The KhES-3 cell line was established at and provided by Kyoto University (Nakatsuji, 2005 (link)). The protocol of this study was reviewed by the Ethics Committee of CiRA in accordance with the "Guidelines for Derivation and Utilization of Human Embryonic Stem Cells" by the Ministry of Education, Culture, Sports, Science and Technology, Japan. The iPS cell lines were established from healthy Japanese donors at CiRA, Kyoto University, and were approved for use by the Ethics Committee of Kyoto University.
Since it has been reported that the toxicity of antioxidants such as catechin is suppressed in the presence of albumin (Zhang et al., 2016 (link)), maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N; Thermo Fisher Scientific). When seeding the cells, 10 μM CultureSure® Y-27632 (FUJIFILM WAKO) was added, and medium exchange on day 1 and thereafter was performed without Y-27632.

Yamane J., Wada T., Otsuki H., Inomata K., Suzuki M., Hisaki T., Sekine S., Kouzuki H., Kobayashi K., Sone H., Yamashita J.K., Osawa M., Saito M.K, & Fujibuchi W. (2022). StemPanTox: A fast and wide-target drug assessment system for tailor-made safety evaluations using personalized iPS cells. iScience, 25(7), 104538.

+ Open protocol

+ Expand

Cell Viability Assay Using CCK-8

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded at a density of 5 × 10⁴ cells per well on 96 well plate coated with Vitronectin (VTN-N, Thermo Fisher). Survival rate was estimated by CCK-8 kit (Dojindo) using Eon microplate spectrophotometer (BioTeK).

Yoo D.H., Im Y.S., Oh J.Y., Gil D, & Kim Y.O. (2023). DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociation. Scientific Reports, 13, 5683.

+ Open protocol

+ Expand

Generation and Validation of EN1 Knockout hPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human pluripotent stem cells [hPSCs; WA09 (H9; 46XX) and MEL1 (46XY)], EN1 knockout H9 hPSCs, and J1 human induced PSC (MRC5), which was previously published in Miller et al., (2015), were grown onto Vitronectin (VTN-N, Thermo Fisher #A14700) coated dishes with Essential 8 media (Life Technologies #A1517001). hPSCs were passaged every 4–5 days by EDTA, and passage 35–55 hPSCs were used for the experiments. For EN1 knockout in hPSCs, guide RNA was predicted with a top score from the CRISPR design tool (http://crispr.mit.edu). Sequence of sgRNA for EN1 knockout was 5-AGCGATGGAGACAGCGTGC-3, and cloned into a CAG-Cas9 2A-GFP U6-sgRNA vector (Addgene, PX458) according to the published instruction (Ran et al., 2013 (link)). 5ug of plasmid was transfected to H9 hPSCs using Nucleofector (Lonza Kit V using the B-016 program). After 48h later, GFP expressed cells were FACS sorted using a BD FACS Aria III in the MSKCC Flow Cytometry core facility followed by growing clonally. Each colony was picked manually, genomic DNA was extracted, and validated EN1 knockout by DNA Sanger sequencing from amplified PCR product of the target region. PCR primers for this are 5-GCCGAGCATGGAAGAACA-3 and 5-CGGGTTCCCAGCTTTAGAC-3. All cell lines are cultured at 37°C with 5% CO2 and routinely tested for mycoplasma.

Kim T.W., Piao J., Koo S.Y., Kriks S., Chung S.Y., Betel D., Socci N.D., Choi S.J., Zabierowski S., Dubose B.N., Hill E.J., Mosharov E.V., Irion S., Tomishima M.J., Tabar V, & Studer L. (2021). Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use. Cell stem cell, 28(2), 343-355.e5.

+ Open protocol

+ Expand

Characterization of Angelman Syndrome hiPSCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Feeder-independent H9 and H1 hESCs (WA09 and WA01) were obtained from WiCell. Neurotypical hiPSCs were purchased from Systems Biosciences (Cat# SC102-A1). Angelman Syndrome (AS) hiPSCs with a class II deletion (AS_del) were developed by the laboratories of Stormy J. Chamberlain and Marc Lalande at the University of Connecticut^{27 (link)} and obtained from Kerafast. H9_{UBE3A m−/p−} and H9_{UBE3A m−/p+} cells with a 66 kb deletion (chr15: 25338949–25405676) and AS point mutation hiPSC line (F583S) (AS_mut) were provided by Dr. Stormy Chamberlain (UCONN). The H9_{UBE3Am−/p−} hESC cell line was generated and validated by Sirois and colleagues^{9 (link)}. Our group also confirmed the deletion in these cells in an earlier study^{15 (link)}. All cell lines were maintained in 6-well tissue culture dishes (Fisher Scientific) coated with 0.5 mg/cm² Vitronectin (VTN-N) (Thermo Fisher) in E8 medium (Thermo Fisher) and passaged using standard protocols. Whole brain human cerebral organoids (hCOs) were generated and maintained using the same protocols as described^{28 (link)}. Cells and hCOs were maintained in a humid incubator at 37 °C with 5% CO₂.

Sen D., Drobna Z, & Keung A.J. (2021). Evaluation of UBE3A antibodies in mice and human cerebral organoids. Scientific Reports, 11, 6323.

+ Open protocol

+ Expand

Culturing Human Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human pluripotent stem cells (hPSCs) were grown in the TeSR-E8 medium (Stem Cell Technologies). Vitronectin (VTN-N, Thermo Fisher) was used as the matrix. Cells were passaged once every 5–7 days using EDTA for chemical passaging or EZPassage (Thermo Fisher) for mechanical passaging. EDTA was added for 3 min. The human induced pluripotent stem cell line, hFSiPS1 (KSCBi002-A), and the human embryonic stem cell line, SNUhES31 (SNUe007-A, established by Seoul National University), used in this study, were obtained from the Korea National Stem Cell Bank^{77 (link),78 (link)}.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!