Commercial O. vulgare essential oil (CO) was purchased from Botanicessence (Product of Spain) (Bangkok, Thailand). Carvacrol, α-tocopherol, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6, tripyridyl-s-triazine (TPTZ), hydrochloric acid (HCl), acetic acid (CH3COOH), linoleic acid, hyaluronidase from bovine testes, sodium chloride (NaCl), collagenase from Clostridium histolyticum, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreas, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAVPN), sodium phosphate monobasic dihydrate (NaH2PO4.2H2O), and sodium phosphate dibasic dihydrate (Na2HPO4.2H2O) were purchased from Sigma-Aldrich (St. Louis, MO, USA); α-Ascorbic acid was purchased from Asia Pacific Specialty Chemicals Limited (New South Wales, Australia). Anhydrous sodium sulfate (Na2SO4), ferric chloride hexahydrate (FeCl3.6H2O), ferrous sulphate heptahydrate (FeSO4.7H2O), and ammonium thiocyanate (NH4SCN) were purchased from Loba Chemie (Boisar, Tarapur, India). Bovine serum albumin was purchased from Merck (Darmstadt, Germany). Tricine was purchased from Bio-Rad Laboratories (Richmond, CA, USA). Sodium acetate trihydrate (CH3COONa.3H2O), calcium chloride dihydrate (CaCl2.2H2O), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), dichloromethane, methanol, and ethanol were analytical grade and purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).
N 3 2 furyl acryloyl leu gly pro ala
Manufactured by Merck Group
Sourced in United States
N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala is a laboratory reagent used for various research applications. It serves as a substrate or component in specific assays or experiments, without providing further details on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
N succinyl ala ala ala p nitroanilide, by Merck Group (4 mentions)
Hyaluronidase, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Epigallocatechin gallate (egcg), by Merck Group (2 mentions)
Linoleic acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by RCI Labscan (1 mentions)
Sodium hydroxide (naoh), by RCI Labscan (1 mentions)
Carvacrol, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Tricine, by Bio-Rad (1 mentions)
Sodium phosphate monobasic dihydrate, by Merck Group (1 mentions)
Sodium phosphate dibasic dihydrate, by Merck Group (1 mentions)
Ethanol, by RCI Labscan (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Tripyridyl s triazine, by Merck Group (1 mentions)
Anhydrous sodium sulfate, by Loba Chemie (1 mentions)
Ferrous sulphate heptahydrate, by Loba Chemie (1 mentions)
4 protocols using n 3 2 furyl acryloyl leu gly pro ala
1
Characterizing Oregano Essential Oil
2
Enzymatic Inhibition Assay for Natural Compounds
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Analytical grade cordycepin (purity ≥98.0%), adenosine (purity ≥99.0%), l -ascorbic acid (purity ≥99.0%), epigallocatechin gallate (EGCG; purity ≥95.0%), oleanolic acid (purity ≥97.0%), sodium chloride (NaCl), sodium phosphate (Na3PO4), sodium dihydrogen phosphate (NaH2PO4), disodium phosphate (Na2HPO4), sodium carbonate (Na2CO3), tricine, tris base, elastase from porcine pancreas lyophilised powder (E–E.C.3.4.21.36), N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), metalloproteinase-1 (MMP-1) from Clostridium histolyticum (Weinberg and Seguin 1916) (ChC–EC.3.4.23.3), N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA; purity ≥99.0%), hyaluronidase from bovine testes (E.C.3.2.1.3.5), hyaluronic acid, bovine serum albumin (BSA), and sodium lauryl sulphate (SLS) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Analytical grade ethanol, hexane, ethyl acetate, acetic acid, and dimethyl sulfoxide (DMSO) were purchased from Labscan (Dublin, Ireland). HPLC-grade methanol was purchased from Labscan (Dublin, Ireland).
3
Antioxidant and Enzyme Inhibition Assays
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2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sunfonic acid) (ABTS), ascorbic acid, Clostridium histolyticum collagenase type I, N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala (FALGPA), porcine pancreas elastase type I, N-Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), epigallocatechin gallate (EGCG), trizma® base, and potassium persulfate were purchased from Sigma–Aldrich; Merck for dimethyl sulfoxide.
4
Cs-4 Mycelium Powder Production and Characterization
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Cs-4 mycelium powder was provided by Jiangxi Guoyao Ltd. (Nanchang, Jiangxi, China). Mushroom tyrosinase, L-tyrosine, 4-Hydroxyphenyl-β-D-glucopyranoside (arbutin), porcine pancreatic elastase, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), collagenase, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), epigallocatechin gallate (EGCG) and edetate disodium were purchased from Sigma-Aldrich, homosalate from Macklin Biochemical (Shanghai), catechin from Yuanye Biology Ltd. (Shanghai).
The Cs-HK1 mycelial biomass was prepared by mycelial liquid fermentation in our lab as reported previously. 25 In brief, Cs-HK1 mycelial biomass maintained on potato dextrose agar medium was inoculated into 50 ml of liquid medium in a 250 ml Erlenmeyer flask and incubated as the starter culture for liquid fermentation. The starter culture broth was transferred into 1 L Erlenmeyer flasks each filled with 250 ml liquid medium (at 4% v/v inoculation ratio) to start the mycelial fermentation. The liquid medium was composed of 40 g/L glucose, 10 g/L yeast extract, 5 g/L peptone, 1 g/L KH 2 PO 4 and 0.5 g/L MgSO47H2O. The liquid culture or mycelial fermentation was operated at 20 C and 150 rpm on a shaker for 7 days. The mycelial biomass was recovered from the liquid broth by centrifugation (6000 rpm, 15 min), and freezedried.
The Cs-HK1 mycelial biomass was prepared by mycelial liquid fermentation in our lab as reported previously. 25 In brief, Cs-HK1 mycelial biomass maintained on potato dextrose agar medium was inoculated into 50 ml of liquid medium in a 250 ml Erlenmeyer flask and incubated as the starter culture for liquid fermentation. The starter culture broth was transferred into 1 L Erlenmeyer flasks each filled with 250 ml liquid medium (at 4% v/v inoculation ratio) to start the mycelial fermentation. The liquid medium was composed of 40 g/L glucose, 10 g/L yeast extract, 5 g/L peptone, 1 g/L KH 2 PO 4 and 0.5 g/L MgSO47H2O. The liquid culture or mycelial fermentation was operated at 20 C and 150 rpm on a shaker for 7 days. The mycelial biomass was recovered from the liquid broth by centrifugation (6000 rpm, 15 min), and freezedried.
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