Rabbit monoclonal antibody (MAb) C29F4 against the HA epitope was purchased from Cell Signaling Technology (Leiden, The Netherlands). Mouse MAbs against HEV ORF2 (1E6), MAVS (Adri-1), GM130 (clone 35), and β-actin (AC-15) were from Millipore (Burlington, MA), AdipoGen (San Diego, CA), BD Biosciences (San Jose, CA), and Sigma-Aldrich (St. Louis, MO), respectively. Mouse MAbs against ERGIC-53 (OTI108), PDI (1D3), and CLIMP63 (G1/296) were from Enzo Life Sciences (Farmingdale, NY), and MAbs against CD63 (MX491295) and CD151 (11G5a) were from Santa Cruz Biotechnology (Dallas, TX). Recombinant mouse antibody MRB198 against genotype 3 HEV ORF3 protein have been described previously (11 (link)). Alexa Fluor 488 and Alexa Fluor 694 anti-mouse IgG as well as Alexa Fluor 594 anti-rabbit IgG secondary antibodies were from Thermo Fisher Scientific. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were from GE Healthcare (Chicago, IL) and Agilent Technologies (Santa Clara, CA), respectively.
Horseradish peroxidase conjugated anti mouse igg and anti rabbit igg secondary antibodies
Manufactured by GE Healthcare
Sourced in United Kingdom
Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies are laboratory reagents used in various immunoassay techniques. These antibodies are designed to bind to and detect the presence of mouse or rabbit primary antibodies, respectively. The horseradish peroxidase (HRP) enzyme conjugated to the secondary antibodies can be used to amplify and visualize the target antigen-antibody interaction.
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Lab products found in correlation
2 protocols using horseradish peroxidase conjugated anti mouse igg and anti rabbit igg secondary antibodies
1
Antibodies for Hepatitis E Virus Detection
2
Evaluating CREB Activation in Bone Marrow-Derived Macrophages
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BMDMs were pretreated without or with H-89 (10 µM; Cell Signaling Technologies)
for 1 h and stimulated with either P. acnes (20 µg/mL) or prostaglandin E2 (PGE2) (10 µM; AdipoGen, Switzerland) alone, or the combination of P. acnes and PGE2 for 0.5 h.
Total cell lysate samples were prepared as previously described [30] . The samples were resolved by SDS-PAGE and transferred to PVDF membranes (PerkinElmer, Boston, MA). The membranes were then incubated with primary antibodies. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Little Chalfont, UK) and used as received.
Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore; Bedford, MA) and an ImageQuant LAS 4000 imaging system (GE Healthcare Japan, Tokyo). The primary antibodies used were anti-phospho-CREB (clone 87G3) and anti-CREB (clone 86B10) antibodies (both from Cell Signaling Technologies) and anti-actin (clone AC15) mAb (Sigma-Aldrich, St.
Louis, MO).
for 1 h and stimulated with either P. acnes (20 µg/mL) or prostaglandin E2 (PGE2) (10 µM; AdipoGen, Switzerland) alone, or the combination of P. acnes and PGE2 for 0.5 h.
Total cell lysate samples were prepared as previously described [30] . The samples were resolved by SDS-PAGE and transferred to PVDF membranes (PerkinElmer, Boston, MA). The membranes were then incubated with primary antibodies. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Little Chalfont, UK) and used as received.
Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore; Bedford, MA) and an ImageQuant LAS 4000 imaging system (GE Healthcare Japan, Tokyo). The primary antibodies used were anti-phospho-CREB (clone 87G3) and anti-CREB (clone 86B10) antibodies (both from Cell Signaling Technologies) and anti-actin (clone AC15) mAb (Sigma-Aldrich, St.
Louis, MO).
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