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Rabbit reticulocyte lysate kit

Manufactured by Promega
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The Rabbit Reticulocyte Lysate Kit provides a cell-free translation system derived from rabbit reticulocytes, which are immature red blood cells. This system allows for the in vitro synthesis of proteins from added mRNA templates.

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Lab products found in correlation

Irdye 800cw streptavidin, by LI COR (1 mentions) Transcend biotin lysyl trna, by Promega (1 mentions) Odyssey fc imaging system, by LI COR (1 mentions)

2 protocols using rabbit reticulocyte lysate kit

1

GST Pull-Down Assay and In Vitro Translation

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GST pull-down assays were performed as described earlier47 (link). The in vitro transcription and translation experiments were performed with a rabbit reticulocyte lysate kit (Promega).
Si W., Zhou J., Zhao Y., Zheng J, & Cui L. (2020). SET7/9 promotes multiple malignant processes in breast cancer development via RUNX2 activation and is negatively regulated by TRIM21. Cell Death & Disease, 11(2), 151.
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2

Biotin-Based Detection of Translation

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In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations. Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products. Samples were assayed by western blot (as above) with the exception that membranes were blocked with 5% BSA/TBS for 1 hour, incubated with IRDye 800CW Streptavidin (LICOR) for 1 hour, washed 3 times and visualised using the LICOR Odyssey Fc imaging system.
Sharp C.P., Thompson B.H., Nash T.J., Diebold O., Pinto R.M., Thorley L., Lin Y.T., Sives S., Wise H., Clohisey Hendry S., Grey F., Vervelde L., Simmonds P., Digard P, & Gaunt E.R. (2023). CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy. PLOS Pathogens, 19(5), e1011357.
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